Mouse Genome Informatics
hm
    E2f1tm1Meg/E2f1tm1Meg
NOD.Cg-E2f1tm1Meg
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
       
cellular
• fractions of cells in S phase from thymus and spleen of 5 week old mutant NOD mice are increased compared with wild-type, fractions of cells in G0-G1 in mutant thymus and spleen are reduced; in the spleen of mutants, number of cells in G2 and M phase are increased relative to wild-type

immune system
• large numbers of mononuclear inflammatory cells have infiltrated the salivary gland of 12-16 week old mutant NOD mice
• thymuses of 5 week old mutant NOD mice are enlarged with respect to wild-type NOD mice with a 55% increase in the number of thymocytes per thymus
• large numbers of mononuclear inflammatory cells have infiltrated the salivary gland of 12-16 week old mutant NOD mice
• numbers of CD4+CD25+ immunoregulatory T cells in the spleen of mutant NOD mice are decreased compared to wild-type NOD mice
• mutant NOD mice have a greater absolute number of speen T cells
• total numbers of thymocytes per thymus and absolute number of cells in all thymocyte populations are significantly increased in null NOD mice compared to wild-type NOD mice
• null animals have lower numbers of double-positive Tcells than wild-type NOD animals
• null animals have lower numbers of double-positive Tcells than wild-type NOD animals
• thymuses of 5 week old null NOD mice contain more mature CD4+ cells than wild-type NOD animals
• thymuses of 5 week old null NOD mice contain more mature CD8+ cells than wild-type NOD animals
• spleen cells from null NOD mice stimulated with anti-CD3 produce increased levels of IFN gamma
• production of IL-4 by spleen cells from null NOD mice is greater than wild-type NOD cells
• incidence of diabetes in knockout female mice is 88% and 58% in male mice, compared with 71% of female wild-type and 25% of male wild-type NOD mice by 32 weeks of age; time of onset is earlier and severity of diabetes is greater in knockouts than wild type littermates
• splenocytes from young mutant NOD mice can induce lympocytic infiltration of the salivary glands and pancreas and induce development of diabetes by spleen T cells from mutant NOD mice transferred into NOD.SCID recipients

endocrine/exocrine glands
• knockout mice on the NOD background have reduced salivary flow rates than NOD or E2f1 knockout controls or the standard (NOD x B10.D2) F1 mice
• large numbers of mononuclear inflammatory cells have infiltrated the salivary gland of 12-16 week old mutant NOD mice
• mononuclear inflammatory cells have infiltrated the pancreas islets of 12-16 week old prediabetic mutant NOD mice; there is some evidence of acinar cell degeneration and abnormally large nuclei or nuclei doubled in number are bserved
• thymuses of 5 week old mutant NOD mice are enlarged with respect to wild-type NOD mice with a 55% increase in the number of thymocytes per thymus
• total numbers of thymocytes per thymus and absolute number of cells in all thymocyte populations are significantly increased in null NOD mice compared to wild-type NOD mice
• large numbers of mononuclear inflammatory cells have infiltrated the salivary gland of 12-16 week old mutant NOD mice

hematopoietic system
• thymuses of 5 week old mutant NOD mice are enlarged with respect to wild-type NOD mice with a 55% increase in the number of thymocytes per thymus
• numbers of CD4+CD25+ immunoregulatory T cells in the spleen of mutant NOD mice are decreased compared to wild-type NOD mice
• mutant NOD mice have a greater absolute number of speen T cells
• total numbers of thymocytes per thymus and absolute number of cells in all thymocyte populations are significantly increased in null NOD mice compared to wild-type NOD mice
• null animals have lower numbers of double-positive Tcells than wild-type NOD animals
• null animals have lower numbers of double-positive Tcells than wild-type NOD animals
• thymuses of 5 week old null NOD mice contain more mature CD4+ cells than wild-type NOD animals
• thymuses of 5 week old null NOD mice contain more mature CD8+ cells than wild-type NOD animals

digestive/alimentary system
• knockout mice on the NOD background have reduced salivary flow rates than NOD or E2f1 knockout controls or the standard (NOD x B10.D2) F1 mice
• large numbers of mononuclear inflammatory cells have infiltrated the salivary gland of 12-16 week old mutant NOD mice

homeostasis/metabolism
• knockout mice on the NOD background have reduced salivary flow rates than NOD or E2f1 knockout controls or the standard (NOD x B10.D2) F1 mice

Mouse Models of Human Disease
OMIM IDRef(s)
Diabetes Mellitus, Insulin-Dependent; IDDM 222100 J:93708
Sjogren Syndrome 270150 J:93708