Phenotypes Associated with This Genotype

Genotype
MGI:3619226

• Allelic Composition: Pik3cg^tm1Pngr/Pik3cg^tm1Pngr
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

thymus hypoplasia ( J:60347 )

• reduction in thymic cellularity

decreased thymocyte number ( J:60347 )

abnormal NK cell differentiation ( J:124330 )

• mice have a higher percentage of CD117+ CD27+ NK cells and lower percentages of 2B4+ NK cells than in splenic NK cells from wild-type mice

abnormal T cell differentiation ( J:60347 )

• T cell development is altered however B cell development is normal

increased eosinophil cell number ( J:60347 )

increased neutrophil cell number ( J:60347 )

decreased CD4-positive, alpha-beta T cell number ( J:60347 )

• decrease in CD4+CD8- T cells in the spleen

increased monocyte cell number ( J:60347 )

abnormal immune cell physiology ( J:60347 )

• thymocytes exhibit an increase in apoptosis compared to wild-type after stimulation with adenosine receptor agonists

abnormal neutrophil physiology ( J:60347 )

• GRCR-induced respiratory burst is decreased in freshly isolated bone marrow neutrophils

impaired neutrophil chemotaxis ( J:60347 )

• 70% decrease in fMLP- and C5a-induced chemotaxis of neutrophils

impaired neutrophil recruitment ( J:60347 )

• accumulation of neutrophils in the peritoneal cavities is reduced in response to casein-induced peritonitis of Listeria infection

abnormal T cell physiology ( J:60347 )

• lymphocytic choriomeningitis virus (LCMV) induced footpad-swelling is reduced, indicating impaired CD+ T cell-dependent antiviral responses

abnormal T-helper 2 physiology ( J:60347 )

• production of antibodies to NIP after NIP-OVA immunization is reduced indicating impairment of functional T helper cell-dependent responses to hapten antigens

decreased cytotoxic T cell cytolysis ( J:124330 )

• NK cells are slightly less lytic than wild-type cells

abnormal T cell activation ( J:60347 )

decreased T cell proliferation ( J:60347 )

• T cells exhibit impaired proliferation in response to anti-CD3 epsilon on Con A stimulation, however exhibit normal proliferation in response to PMA/Ca+ inonaphore

decreased interferon-gamma secretion ( J:60347 , J:124330 )

• T cells produce lower amounts of interferon-gamma in response to treatment with anti-CD3 epsilon and anti-CD28, Con A, or PMA/Ca+ ionaphore (J:60347)

• mice exhibit decreased IFN-gamma secretion following NK1.1 or IL-12/IL-18 stimulation compared to in wild-type mice (J:124330)

decreased interleukin-2 secretion ( J:60347 )

• T cells produce lower amounts of IL-2 in response to treatment with anti-CD3 epsilon and anti-CD28, Con A, or PMA/Ca+ ionaphore

hematopoietic system

thymus hypoplasia ( J:60347 )

• reduction in thymic cellularity

decreased thymocyte number ( J:60347 )

abnormal definitive hematopoiesis ( J:60347 )

• increase in total and relative numbers of Gr1+Mac1+ myeloid cells in the spleen but not in bone marrow

abnormal NK cell differentiation ( J:124330 )

• mice have a higher percentage of CD117+ CD27+ NK cells and lower percentages of 2B4+ NK cells than in splenic NK cells from wild-type mice

abnormal T cell differentiation ( J:60347 )

• T cell development is altered however B cell development is normal

increased eosinophil cell number ( J:60347 )

increased neutrophil cell number ( J:60347 )

decreased CD4-positive, alpha-beta T cell number ( J:60347 )

• decrease in CD4+CD8- T cells in the spleen

increased monocyte cell number ( J:60347 )

abnormal neutrophil physiology ( J:60347 )

• GRCR-induced respiratory burst is decreased in freshly isolated bone marrow neutrophils

impaired neutrophil chemotaxis ( J:60347 )

• 70% decrease in fMLP- and C5a-induced chemotaxis of neutrophils

impaired neutrophil recruitment ( J:60347 )

• accumulation of neutrophils in the peritoneal cavities is reduced in response to casein-induced peritonitis of Listeria infection

abnormal T cell physiology ( J:60347 )

• lymphocytic choriomeningitis virus (LCMV) induced footpad-swelling is reduced, indicating impaired CD+ T cell-dependent antiviral responses

abnormal T-helper 2 physiology ( J:60347 )

• production of antibodies to NIP after NIP-OVA immunization is reduced indicating impairment of functional T helper cell-dependent responses to hapten antigens

decreased cytotoxic T cell cytolysis ( J:124330 )

• NK cells are slightly less lytic than wild-type cells

abnormal T cell activation ( J:60347 )

decreased T cell proliferation ( J:60347 )

• T cells exhibit impaired proliferation in response to anti-CD3 epsilon on Con A stimulation, however exhibit normal proliferation in response to PMA/Ca+ inonaphore

cellular

impaired neutrophil chemotaxis ( J:60347 )

• 70% decrease in fMLP- and C5a-induced chemotaxis of neutrophils

decreased T cell proliferation ( J:60347 )

• T cells exhibit impaired proliferation in response to anti-CD3 epsilon on Con A stimulation, however exhibit normal proliferation in response to PMA/Ca+ inonaphore

endocrine/exocrine glands

thymus hypoplasia ( J:60347 )

• reduction in thymic cellularity

decreased thymocyte number ( J:60347 )