Phenotypes Associated with This Genotype

Genotype
MGI:3580160

• Allelic Composition: Lta^tm1Dch/Lta^tm1Dch
• Genetic Background: involves: 129S2/SvPas * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

increased susceptibility to induced morbidity/mortality ( J:109621 )

• in response to LPS and D-GalN administration, percentage of severely moribund mice is 0 at 7 hours; 3/7 mice are severely moribund at 8 hours

immune system

abnormal class switch recombination ( J:109621 )

• 19 days after immunization with OVA, mice display an isotype switch to IgG from IgM that is several fold decreased compared to wild-type

abnormal leukocyte cell number ( J:17976 )

decreased T cell number ( J:17976 )

• in the spleen

increased leukocyte cell number ( J:17976 )

• nearly 3 fold increase

• neutrophil, monocyte, platelet counts normal

increased B cell number ( J:17976 )

• 4 fold increase in B cell numbers

• 30% increase in IgM positive and B220 positive B lymphocytes

abnormal spleen morphology ( J:17976 , J:39746 )

• mice exhibit extensive disorganization (J:39746)

absent spleen marginal zone ( J:17976 )

• loss of a distinct marginal zone

abnormal spleen periarteriolar lymphoid sheath morphology ( J:17976 )

decreased spleen white pulp amount ( J:109621 )

• percentage of splenic white pulp is lower than in wild-type, Ltb nulls and Lta^tm1.1Sned but higher than Ltb, Ltbr or Ltb/Tnf/Lta knockouts

absent Peyer's patches ( J:17976 )

• not grossly detectable

abnormal lymph node morphology ( J:17976 )

• lack para-aortic lymph nodes

absent mesenteric lymph nodes ( J:17976 , J:39746 )

abnormal lymph node primary follicle morphology ( J:109621 )

• only occasional BP-3 cells are found in some lymphoid follicles

absent axillary lymph nodes ( J:17976 )

absent cervical lymph nodes ( J:17976 )

absent inguinal lymph nodes ( J:17976 )

absent popliteal lymph nodes ( J:17976 )

abnormal humoral immune response ( J:39746 )

• in response to stimulation with sheep red blood cells (SRBC), mice fail to produce anti-SRBC IgG at day 6 and only produce a negligible amount by day 15 unlike wild-type mice

decreased interferon-gamma secretion ( J:109621 )

• in culture, OVA-specific Ifng production from purified CD4+ T cells is decreased 2-fold compared to Lta^tm1.1Sned

decreased tumor necrosis factor secretion ( J:109621 )

• in response to LPS, mice demonstrate a 10-fold decrease in production of TNF compared to wild-type or Lta^tm1.1Sned

• both bone marrow derived macrophages and neutrophils fail to produce normal levels of TNF when stimulated

hematopoietic system

abnormal class switch recombination ( J:109621 )

• 19 days after immunization with OVA, mice display an isotype switch to IgG from IgM that is several fold decreased compared to wild-type

abnormal leukocyte cell number ( J:17976 )

decreased T cell number ( J:17976 )

• in the spleen

increased leukocyte cell number ( J:17976 )

• nearly 3 fold increase

• neutrophil, monocyte, platelet counts normal

increased B cell number ( J:17976 )

• 4 fold increase in B cell numbers

• 30% increase in IgM positive and B220 positive B lymphocytes

abnormal spleen morphology ( J:17976 , J:39746 )

• mice exhibit extensive disorganization (J:39746)

absent spleen marginal zone ( J:17976 )

• loss of a distinct marginal zone

abnormal spleen periarteriolar lymphoid sheath morphology ( J:17976 )

decreased spleen white pulp amount ( J:109621 )

• percentage of splenic white pulp is lower than in wild-type, Ltb nulls and Lta^tm1.1Sned but higher than Ltb, Ltbr or Ltb/Tnf/Lta knockouts