Phenotypes Associated with This Genotype

Genotype
MGI:3056062

• Allelic Composition: Btrc^tm1Paga/Btrc^tm1Paga
• Genetic Background: involves: 129S2/SvPas

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

mortality/aging ( J:93010 )

N • homozygous mice appeared generally as healthy as wildtype littermates over 2 years' observation

N • almost all mutants revealed no gross tissue abnormalities upon necropsy

N • exceptions were a single invasive intestinal carcinoma in a 10 month-old mouse and death due to thymic lymphoma of 2 mice aged 6.5 and 9 months

cellular

oligozoospermia ( J:93010 )

♂ • histological examination of epidydimes of homozygous males revealed severely diminished numbers of mature spermatozoa

abnormal centrosome morphology ( J:93010 )

• approximately 20% of asynchronously dividing MEFs were found to have supernumerary centrosomes

abnormal mitosis ( J:93010 )

• cultured homozygous mouse embryonic fibroblasts (MEFs) were retarded in their progression through mitosis, despite normal passage from G1 to S phase

• MEFs exhibited misalignment of chromosomes at the metaphase plate

• approximately 20% of asynchronously dividing MEFs were found to have supernumerary centrosomes, and approximately 12% of dividing MEFs exhibited multipolar spindles

abnormal mitotic spindle morphology ( J:93010 )

• approximately 12% of dividing MEFs exhibited multipolar spindles

reproductive system

oligozoospermia ( J:93010 )

♂ • histological examination of epidydimes of homozygous males revealed severely diminished numbers of mature spermatozoa

abnormal male meiosis ( J:93010 )

♂ • histological examination of epidydimes of homozygous males revealed evidence of prolonged, abnormal meiosis

♂ • numbers of metaphase I spermatocytes were elevated

♂ • in some mice, spindle abnormalities and misaligned chromosomes were observed

♂ • the degree of fertility impairment correlated with the severity of histologic abnormality

abnormal meiotic spindle morphology ( J:93010 )

• in some spermatocytes, spindle abnormalities and misaligned chromosomes were observed

reduced male fertility ( J:93010 )

♂ • approximately 50% of homozygous male mice produced no progeny when paired with wild-type females

♂ • fertility defect was not behavioral in origin, as mating behavior was normal and vaginal plugs were observed

♂ • productive homozygous males sired fewer and smaller litters, on average, than did wild-type males