Phenotypes Associated with This Genotype

Genotype
MGI:2668994

• Allelic Composition: Tlx1^tm1Sjk/Tlx1^tm1Sjk
• Genetic Background: involves: 129S2/SvPas

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

increased number of Howell-Jolly bodies ( J:17695 )

• many erythrocytes contain nuclear fragments of condensed DNA known as Howell-Jolly bodies, commonly associated with asplenia

• however, complete blood counts indicate that the number of red blood cells is normal

increased leukocyte cell number ( J:17695 )

• homozygotes display a ~2-fold increase in the number of white blood cells relative to wild-type mice

• however, the B-cell and T-cell profile is normal in thymus, lymph node and peripheral blood

increased neutrophil cell number ( J:17695 )

• in peripheral blood smears

increased lymphocyte cell number ( J:17695 )

• in peripheral blood smears

abnormal spleen development ( J:17695 )

• at E12.5, mutant embryos display no cellular organization or mesenchymal cell condensation at the site of splenic development

• at E13.5, mutant embryos show no histological evidence of a splenic primordium within the dorsal mesogastrium

abnormal spleen mesenchyme morphology ( J:17695 )

• at E12.5, mutant embryos display no mesenchymal cell condensation at the site of splenic development, indicating a specific, localized defect in mesodermal cells destined to form the spleen

absent spleen ( J:17695 )

• all adult homozygotes appear externally normal but display complete absence of spleen

• in contrast, all other internal organs (including the stomach and pancreas) are normal in morphology and position

immune system

increased leukocyte cell number ( J:17695 )

• homozygotes display a ~2-fold increase in the number of white blood cells relative to wild-type mice

• however, the B-cell and T-cell profile is normal in thymus, lymph node and peripheral blood

increased neutrophil cell number ( J:17695 )

• in peripheral blood smears

increased lymphocyte cell number ( J:17695 )

• in peripheral blood smears

abnormal spleen development ( J:17695 )

• at E12.5, mutant embryos display no cellular organization or mesenchymal cell condensation at the site of splenic development

• at E13.5, mutant embryos show no histological evidence of a splenic primordium within the dorsal mesogastrium

abnormal spleen mesenchyme morphology ( J:17695 )

• at E12.5, mutant embryos display no mesenchymal cell condensation at the site of splenic development, indicating a specific, localized defect in mesodermal cells destined to form the spleen

absent spleen ( J:17695 )

• all adult homozygotes appear externally normal but display complete absence of spleen

• in contrast, all other internal organs (including the stomach and pancreas) are normal in morphology and position

embryo

embryo phenotype ( J:17695 )

N • despite observed gene expression in the branchial region of wild-type mice, homozygotes display no detectable abnormalities in branchial arches

nervous system

nervous system phenotype ( J:17695 )

N • despite observed gene expression in the developing hindbrain and cranial ganglia of wild-type mice, homozygotes display no detectable abnormalities in the hindbrain, cranial ganglia or cranial motor nuclei