Phenotypes Associated with This Genotype

Genotype
MGI:2664241

• Allelic Composition: Mybl1^tm1Epr/Mybl1^tm1Epr
• Genetic Background: involves: 129 * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased B cell number ( J:67604 )

• ~2-fold reduction in percentage of splenic B cells (B220⁺) relative to wild-type controls; however, proportions of splenic immature and mature B cells are normal

• normal B cell development in the bone marrow, as shown by normal numbers of pro-B, pre-B, immature, and mature B cell subpopulations

increased spleen red pulp amount ( J:67604 )

• expanded red pulp areas

small spleen ( J:67604 )

• reduced average spleen size

spleen hypoplasia ( J:67604 )

• mild splenic hypoplasia involving all areas of the white pulp

abnormal spleen B cell follicle morphology ( J:67604 )

• mildly hypoplastic

decreased spleen germinal center number ( J:67604 )

• the number of "spontaneous" GCs in naive mutant spleens is severalfold lower than that in wild-type controls

abnormal spleen marginal zone morphology ( J:67604 )

• mildly hypoplastic

abnormal humoral immune response ( J:67604 )

• impaired primary serum antibody response, following immunization with the T cell-dependent antigen NP-CGG in alum, as shown by reduced primary serum levels of IgG3 and IgG1 (the major isotypes in this response), as well as IgG2a; however, the early primary IgM response and secondary anti-NP serum Ab responses are normal

• normal germinal center reaction following immunization with the T cell-dependent antigen NP-CGG

• normal development of B cell memory, as shown by normal V gene somatic hypermutation and Ag affinity-based positive selection during the anti-NP response

• normal rate of B cell proliferation following in vitro stimulation with LPS, anti-CD40, or anti-IgM

• normal H chain class switching by B cells following in vitro stimulation with LPS or LPS and IL-4

decreased IgG1 level ( J:67604 )

• reduced primary serum levels of IgG1 following immunization with the T cell-dependent antigen NP-CGG

decreased IgG2a level ( J:67604 )

• reduced primary serum levels of IgG2a following immunization with the T cell-dependent antigen NP-CGG

decreased IgG3 level ( J:67604 )

• reduced primary serum levels of IgG3 following immunization with the T cell-dependent antigen NP-CGG

hematopoietic system

decreased B cell number ( J:67604 )

• ~2-fold reduction in percentage of splenic B cells (B220⁺) relative to wild-type controls; however, proportions of splenic immature and mature B cells are normal

• normal B cell development in the bone marrow, as shown by normal numbers of pro-B, pre-B, immature, and mature B cell subpopulations

increased spleen red pulp amount ( J:67604 )

• expanded red pulp areas

small spleen ( J:67604 )

• reduced average spleen size

spleen hypoplasia ( J:67604 )

• mild splenic hypoplasia involving all areas of the white pulp

abnormal spleen B cell follicle morphology ( J:67604 )

• mildly hypoplastic

decreased spleen germinal center number ( J:67604 )

• the number of "spontaneous" GCs in naive mutant spleens is severalfold lower than that in wild-type controls

abnormal spleen marginal zone morphology ( J:67604 )

• mildly hypoplastic

decreased IgG1 level ( J:67604 )

• reduced primary serum levels of IgG1 following immunization with the T cell-dependent antigen NP-CGG

decreased IgG2a level ( J:67604 )

• reduced primary serum levels of IgG2a following immunization with the T cell-dependent antigen NP-CGG

decreased IgG3 level ( J:67604 )

• reduced primary serum levels of IgG3 following immunization with the T cell-dependent antigen NP-CGG