Phenotypes Associated with This Genotype

Genotype
MGI:2653297

• Allelic Composition: Cd5l^tm1Nto/Cd5l^tm1Nto
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

decreased thymocyte number ( J:52195 )

• the CD4/CD8 double-positive (DP) thymocyte numbers in homozygous null mice are reduced to less than half the number present in wild-type control mice, whereas the numbers of either CD4/CD8 double-negative (DN) immature thymocytes or mature single-positive (SP) thymocytes remain grossly normal

abnormal double-positive T cell morphology ( J:52195 )

• in homozygous null mice, DP thymocytes exhibit a strikingly increased susceptibility to cell death in response to both dexamethasone and irradiation due to massive apoptosis in the cortex (confirmed by TUNEL analysis)

• in vitro, recombinant CD5 antigen-like protein significantly inhibits apoptosis of DP thymocytes as well as CD95/Fas-mediated apoptosis of a monocyte-derived cell line, J774A.1, which shows high binding capacity for CD5L

immune system

immune system phenotype ( J:52195 )

N • thymocyte development from the DN to the DP stage appears normal

decreased thymocyte number ( J:52195 )

• the CD4/CD8 double-positive (DP) thymocyte numbers in homozygous null mice are reduced to less than half the number present in wild-type control mice, whereas the numbers of either CD4/CD8 double-negative (DN) immature thymocytes or mature single-positive (SP) thymocytes remain grossly normal

abnormal double-positive T cell morphology ( J:52195 )

• in homozygous null mice, DP thymocytes exhibit a strikingly increased susceptibility to cell death in response to both dexamethasone and irradiation due to massive apoptosis in the cortex (confirmed by TUNEL analysis)

• in vitro, recombinant CD5 antigen-like protein significantly inhibits apoptosis of DP thymocytes as well as CD95/Fas-mediated apoptosis of a monocyte-derived cell line, J774A.1, which shows high binding capacity for CD5L

increased susceptibility to bacterial infection ( J:82352 )

• when injected with heat-killed Corynebacterium parvum, homozygous null mice display enhanced and prolonged bacterial-induced granuloma formation

• mutant mice exhibit increased apoptosis of natural killer T (NKT) and T cells in the early stage of hepatic granuloma formation, and delayed repopulation of NKT cells in the late stage of granuloma formation

• following C. parvum injection, most of the cytokine mRNA expression patterns are comparable between wild-type and homozygous null mice; however, IL-10 mRNA expression is less pronounced, whereas IL-12 and chemokine (C-C motif) ligand 2 (CCL2) mRNA expressions are enhanced in the liver of homozygous null mice compared with control mice

• in vitro, recombinant protein rescues the apoptosis of NKT and T cells obtained from C. parvum-injected hepatic mononuclear cells

endocrine/exocrine glands

decreased thymocyte number ( J:52195 )

• the CD4/CD8 double-positive (DP) thymocyte numbers in homozygous null mice are reduced to less than half the number present in wild-type control mice, whereas the numbers of either CD4/CD8 double-negative (DN) immature thymocytes or mature single-positive (SP) thymocytes remain grossly normal