Phenotypes Associated with This Genotype

Genotype
MGI:2174981

• Allelic Composition: Nos3^tm1Unc/Nos3^tm1Unc
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

decreased susceptibility to induced morbidity/mortality ( J:113106 )

• after injection of platelet-activating factor (PAF), less than 10% mortality occurs within 30 minutes

• pretreatment with wortmannin before PAF treatment confers 100% protection to mutants and wild-type

• in BSA-induced anaphylaxis, all mutants survive compared to fatality in 82% of controls; in OVA model, 92% of control mice die but all mutants survive challenge

growth/size/body

decreased body weight ( J:36559 , J:101254 )

• body weights at 14 weeks of age are approximately 7.5% lower than in wild-type (J:36559)

• body weight is signifcantly lower than controls at 14 days of age (J:101254)

homeostasis/metabolism

increased circulating renin level ( J:36559 )

• plasma renin concentrations are nearly twice as high as in wild-type

decreased body temperature ( J:113106 )

• challenge with BSA 2 weeks after sensitization with BSA induces severe hypothermia and mice succumb to systemic shock reaction; mutants show no mortality and only a delayed, mild, transient hypothermia

• similar results are seen with OVA-induced anaphylaxis

cardiovascular system

abnormal cardiovascular system morphology ( J:101254 )

abnormal pulmonary artery morphology ( J:101254 )

• larger than in controls but only at 14 day

• medial wall area is greater than in controls in the fetus

• muscularity decreases after birth

• muscularity is reduced in the male but still greater than controls

• muscularity in females is like controls by 14 days of age

abnormal heart weight ( J:101254 )

• right ventricle/body weight ratio increased in males

• right ventricle/left ventricle+septum ratio increased in males

abnormal cardiovascular system physiology ( J:101254 )

abnormal heart right ventricle pressure ( J:101254 )

• mean right ventricular pressure is elevated in males but not in females

decreased heart rate ( J:36559 )

• 670 beats per min vs. 709 beats per min in wild-type

increased systemic arterial blood pressure ( J:36559 )

increased mean systemic arterial blood pressure ( J:101254 )

• elevated in males but not in females

decreased vascular permeability ( J:113106 )

• in BSA/BSA model of anaphylaxis, vascular permeability increased significantly in wild-type but very little in mutants; vascular leakage (extravasation) of Evans Blue dye (EB) is 2-fold lower than in wild-type mice

• in OVA/OVA model, no vascular permeability increase occurs in mutants and extravasation is significantly lower than in wild-type

immune system

immune system phenotype ( J:36559 )

N • exhibit a similar susceptibility to lipopolysaccharide-induced death as wild-type