Phenotypes for Fn1 MGI:2168531 MGI Mouse

embryonic lethality during organogenesis, complete penetrance ( J:16247 )

• homozygotes develop severe embryonic abnormalities from E8.0 onwards and undergo degeneration during E10 and E11

abnormal dorsal aorta morphology ( J:16247 )

• at E8.5, severely affected embryos show absence of dorsal aortae while less severely affected embryos exhibit distended dorsal aortae containing only a few blood cells

abnormal vasculogenesis ( J:16247 )

• at E8.5, homozygotes display defective vasculogenesis in the yolk sac

• mutant embryonic vessels appear variable and deformed while extraembryonic vasculature is also defective

absent vitelline blood vessels ( J:16247 )

• at E8.5, blood vessels are detected in the exocoelomic cavity rather than in the yolk sac

thick myocardium ( J:16247 )

• at E8.5, homozygotes display a thickened myocardium

abnormal heart development ( J:16247 )

• at E8.5, 13 of 20 mutant embryos have a visible primitive heart whereas the remaining seven do not

• in severely affected embryos, heart primordia fail to fuse and are positioned laterally

decreased cardiac jelly amount ( J:16247 )

• at E8.5, cardiac jelly is deficient

abnormal endocardium morphology ( J:16247 )

• at E8.5, the mutant endocardium is either indistinguishable from the thickened myocardium or absent

abnormal developmental patterning ( J:16247 )

failure of initiation of embryo turning ( J:16247 )

• at E8.5, none of the mutant embryos have initiated turning

abnormal mesoderm development ( J:16247 )

• at E8.0, homozygotes exhibit a deficit in the trunk and headfold mesoderm

• by E8.5, mutant headfolds appear small and misshapen, with a deficit in underlying mesoderm and many pyknotic cells

short rostral-caudal axis ( J:16247 )

• at E8.0, homozygotes display a shortened anterior-posterior axis

embryonic growth retardation ( J:16247 )

• starting at E8.0, homozygotes appear developmentally retarded and abnormal

decreased embryo size ( J:16247 )

• at E7.5, mutant embryos appear relatively normal, though slightly smaller than wild-type embryos

abnormal embryonic tissue morphology ( J:16247 )

abnormal lateral plate mesoderm morphology ( J:16247 )

• by E8.5, lateral mesoderm flanking the neural tube is reduced

abnormal neural tube morphology ( J:16247 )

abnormal embryonic neuroepithelium morphology ( J:16247 )

• at E8.0, homozygotes display multiple bends and distortions in the neural ectoderm

kinked neural tube ( J:16247 )

• at E8.5, homozygotes display a kinked neural tube

absent notochord ( J:16247 )

• at E8.5, homozygotes lack an organized notochord; instead, the endodermal lining of the future midgut is juxtaposed to the neural tube

absent somites ( J:16247 )

• at E8.5, mutant embryos lack somites whereas wild-type embryos contain 8 to 12 pairs of somites; however, condensations of cells suggestive of incipient somites are detected in 3 of 20 mutants

abnormal extraembryonic tissue morphology ( J:16247 )

absent vitelline blood vessels ( J:16247 )

• at E8.5, blood vessels are detected in the exocoelomic cavity rather than in the yolk sac

abnormal amnion morphology ( J:16247 )

• at E7.5, mutant embryos possess three germ layers and normal extraembryonic membranes except for a concave amnion

• at E8.0, the mutant amnion is undersized and closely apposed to the embryo, displaying a pressure deficit in the amniotic cavity

abnormal visceral yolk sac morphology ( J:16247 )

• at E8.5, the mesodermal and endodermal layers of the mutant yolk sac appear to split apart

absent visceral yolk sac blood islands ( J:16247 )

failure of chorioallantoic fusion ( J:16247 )

• at E8.5, the allantois has yet not fused with the chorion