Phenotypes associated with this allele

• Allele Symbol: Ccl28^tm1Takna
• Allele Name: targeted mutation 1, Takashi Nakayama
• Allele ID: MGI:6367826
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ccl28^tm1Takna/Ccl28^tm1Takna	C57BL/6J-Ccl28^tm1Takna	MGI:6367828

Genotype
MGI:6367828

hm1

• Allelic Composition: Ccl28^tm1Takna/Ccl28^tm1Takna
• Genetic Background: C57BL/6J-Ccl28^tm1Takna

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

increased susceptibility to induced colitis ( J:256896 )

• mice exhibit aggravated DSS-induced colitis, showing increased weight loss, decreased colon length, enhanced mucosal inflammation and tissue damage in the colon

• mice with DSS-induced colitis show bacterial cells colonized in the lamina propria and in the muscularis of the colon, indicating that the colon mucosa is less well protected from bacterial invasion upon intestinal injury

• mice pretreated with a potent antibiotic mixture and then treated with DSS show almost completely ameliorated DSS-induced colitis

decreased IgA level ( J:256896 )

• mice exhibit a drastic reduction in total and antigen-specific IgA levels in feces

• serum IgA levels are reduced by about 50%

• IgA antibody-secreting cells from the colon, but not the small intestine, show reduced secretion of IgA, with the average amount of IgA secreted by a single cell reduced by about 70%

increased IgG level ( J:256896 )

• serum IgG levels are increased by about 2-fold

• however, fecal IgG levels are similar to wild-type mice and serum and fecal antigen-specific IgG levels are normal

abnormal immune system cell morphology ( J:256896 )

• mice exhibit a reduction of IgA antibody-secreting cells in mucosal tissues (colon, sublingual gland)

• while in wild-type mice, IgA antibody-secreting cells are localized in the area adjacent to the muscularis mucosae and in the upper lamina propria of the colon, in mutant mice they are primarily only seen in the area adjacent to the muscularis mucosae

hematopoietic system

decreased IgA level ( J:256896 )

• mice exhibit a drastic reduction in total and antigen-specific IgA levels in feces

• serum IgA levels are reduced by about 50%

• IgA antibody-secreting cells from the colon, but not the small intestine, show reduced secretion of IgA, with the average amount of IgA secreted by a single cell reduced by about 70%

increased IgG level ( J:256896 )

• serum IgG levels are increased by about 2-fold

• however, fecal IgG levels are similar to wild-type mice and serum and fecal antigen-specific IgG levels are normal

digestive/alimentary system

abnormal colon morphology ( J:256896 )

• IgA antibody-secreting cells in the lamina propria are reduced by about 50% in the colon but remain at normal levels in the small intestine

• however, no differences in levels of CD4+ T cells or CD8+ T cells in the colon and no differences in the frequency of IgA+ cells in total B220+ cells of Peyers and cecal patches

abnormal gut flora balance ( J:256896 )

• abundance of Class Bacilli bacteria is increased in the intestine

increased susceptibility to induced colitis ( J:256896 )

• mice exhibit aggravated DSS-induced colitis, showing increased weight loss, decreased colon length, enhanced mucosal inflammation and tissue damage in the colon

• mice with DSS-induced colitis show bacterial cells colonized in the lamina propria and in the muscularis of the colon, indicating that the colon mucosa is less well protected from bacterial invasion upon intestinal injury

• mice pretreated with a potent antibiotic mixture and then treated with DSS show almost completely ameliorated DSS-induced colitis

cellular

abnormal cell migration ( J:256896 )

• mutant mice transferred with wild-type splenocytes show fewer labeled IgA antibody-secreting cells in the colon and IgA antibody-secreting cells are not seen in the upper lamina propria of the mutant colon indicating that homing of IgA antibody-secreting cells to the colon is impaired