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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Gt(ROSA)26Sortm1(OVAL/fla,GFP)Vnce
targeted mutation 1, Russell E Vance
MGI:6196853
Summary 5 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Gt(ROSA)26Sortm1(OVAL/fla,GFP)Vnce/Gt(ROSA)26Sor+
Lyz2tm1(cre)Ifo/Lyz2+
Pycardtm1Vmd/Pycardtm1Vmd
involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6J MGI:6196858
cn2
Gt(ROSA)26Sortm1(OVAL/fla,GFP)Vnce/Gt(ROSA)26Sor+
Lyz2tm1(cre)Ifo/Lyz2+
involves: 129P2/OlaHsd * C57BL/6J MGI:6196856
cn3
Gt(ROSA)26Sortm1(OVAL/fla,GFP)Vnce/Gt(ROSA)26Sor+
Lyz2tm1(cre)Ifo/Lyz2+
Nlrc4tm1Vmd/Nlrc4tm1Vmd
involves: 129P2/OlaHsd * C57BL/6J * C57BL/6NCrl MGI:6196855
cn4
Gt(ROSA)26Sortm1(OVAL/fla,GFP)Vnce/Gt(ROSA)26Sor+
Tg(S100A8-cre,-EGFP)1Ilw/0
involves: C3H * C57BL/6 * C57BL/6J MGI:6196860
cn5
Gt(ROSA)26Sortm1(OVAL/fla,GFP)Vnce/Gt(ROSA)26Sor+
Tg(Itgax-cre)1-1Reiz/0
involves: C3H * C57BL/6 * C57BL/6J MGI:6196862


Genotype
MGI:6196858
cn1
Allelic
Composition
Gt(ROSA)26Sortm1(OVAL/fla,GFP)Vnce/Gt(ROSA)26Sor+
Lyz2tm1(cre)Ifo/Lyz2+
Pycardtm1Vmd/Pycardtm1Vmd
Genetic
Background
involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1(OVAL/fla,GFP)Vnce mutation (0 available); any Gt(ROSA)26Sor mutation (758 available)
Lyz2tm1(cre)Ifo mutation (13 available); any Lyz2 mutation (28 available)
Pycardtm1Vmd mutation (1 available); any Pycard mutation (6 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
growth/size/body
N
• mice show normal weight gain at 10 weeks of age

homeostasis/metabolism
• slight increase in IFN-gamma levels
• slight increase in IL-6 levels
• slight increase in TNF levels

immune system
N
• mice show no joint inflammation at 10 weeks of age, cytokine levels (such as IL-1 alpha, IL-1 beta, and MCP-1) are severely reduced, and the autoinflammatory disease seen in Gt(ROSA)26Sortm1(OVAL/fla-GFP)Vnce and Lyz2tm1(cre)Ifo expressing mice is almost entirely ameliorated
• slight increase in IFN-gamma levels
• slight increase in IL-6 levels
• slight increase in TNF levels




Genotype
MGI:6196856
cn2
Allelic
Composition
Gt(ROSA)26Sortm1(OVAL/fla,GFP)Vnce/Gt(ROSA)26Sor+
Lyz2tm1(cre)Ifo/Lyz2+
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1(OVAL/fla,GFP)Vnce mutation (0 available); any Gt(ROSA)26Sor mutation (758 available)
Lyz2tm1(cre)Ifo mutation (13 available); any Lyz2 mutation (28 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
growth/size/body
• mice are runted prior to weaning
• mice exhibit lower body weights throughout life
• mice treated with an alpha IL-1R blocking antibody show weight gain
• sick mice exhibit enlarged spleen

digestive/alimentary system
• pronounced neutrophilia is seen in the duodenums, with neutrophilic infiltration of the submucosa and lamina propria that extends into the underlying muscularis externa

hematopoietic system
• sick mice exhibit enlarged spleen
• pronounced neutrophilia is seen in the joints, duodenums, and kidney glomeruli
• increase in neutrophils in the peripheral blood of sick mice compared to healthy littermates
• increase in levels of neutrophils in spleen, and in peripheral and mesenteric lymph nodes
• mice treated with an alpha IL-1R blocking antibody show decreased levels of neutrophils in both the spleen and lymph nodes
• slight increase of T cells in the spleen, however there are no changes in T cells in the lymph nodes
• decrease in blood lymphocytes
• B cell numbers are modestly decreased in the lymph nodes
• increase in monocytes in the peripheral blood of the sick mice compared to healthy littermates
• increase in levels of monocytes in spleen, and in peripheral and mesenteric lymph nodes
• mice treated with an alpha IL-1R blocking antibody show decreased levels of monocytes in both the spleen and lymph nodes

homeostasis/metabolism
• serum levels of cytokines and chemokines (IL-1 alpha, IL-1 beta, IFN-gamma, IL-6, MCP-1) are elevated
• mice treated with an alpha IL-1R blocking antibody show decreased IL-1bbeta, MCP-1, and TNF levels, however, levels of IL-18 are unchanged
• mice develop severe limb swelling, most noticeably in the tibiotarsal (heel) joint
• tibiotarsal joint swelling is 100% penetrant, although age of onset varies, beginning as early as 4 weeks or as late as 10 weeks of age
• substantial neutrophilic infiltration with slight to severe bone and cartilage erosion is seen in the tibiotarsal joint
• mice treated with an alpha IL-1R blocking antibody show decreased tibiotarsal joint swelling

immune system
• sick mice exhibit enlarged spleen
• pronounced neutrophilia is seen in the joints, duodenums, and kidney glomeruli
• increase in neutrophils in the peripheral blood of sick mice compared to healthy littermates
• increase in levels of neutrophils in spleen, and in peripheral and mesenteric lymph nodes
• mice treated with an alpha IL-1R blocking antibody show decreased levels of neutrophils in both the spleen and lymph nodes
• slight increase of T cells in the spleen, however there are no changes in T cells in the lymph nodes
• decrease in blood lymphocytes
• B cell numbers are modestly decreased in the lymph nodes
• increase in monocytes in the peripheral blood of the sick mice compared to healthy littermates
• increase in levels of monocytes in spleen, and in peripheral and mesenteric lymph nodes
• mice treated with an alpha IL-1R blocking antibody show decreased levels of monocytes in both the spleen and lymph nodes
• serum levels of cytokines and chemokines (IL-1 alpha, IL-1 beta, IFN-gamma, IL-6, MCP-1) are elevated
• mice treated with an alpha IL-1R blocking antibody show decreased IL-1bbeta, MCP-1, and TNF levels, however, levels of IL-18 are unchanged
• sick mice exhibit enlarged lymph nodes
• mice exhibit systemic inflammation, with nearly all tissues showing neutrophilic infiltration
• pronounced neutrophilia is seen in the duodenums, with neutrophilic infiltration of the submucosa and lamina propria that extends into the underlying muscularis externa
• substantial neutrophilic infiltration with slight to severe bone and cartilage erosion is seen in the tibiotarsal joint
• mice show varying degrees of kidney damage, with some mice having fibrin and neutrophil accumulation within the glomeruli indicating necrotizing glomerulonephritis, while others have some glomeruli with only thickened membranes

renal/urinary system
• some mice exhibit glomeruli with only thickened membranes
• mice show varying degrees of kidney damage, with some mice having fibrin and neutrophil accumulation within the glomeruli indicating necrotizing glomerulonephritis, while others have some glomeruli with only thickened membranes

skeleton
• mice develop severe limb swelling, most noticeably in the tibiotarsal (heel) joint
• tibiotarsal joint swelling is 100% penetrant, although age of onset varies, beginning as early as 4 weeks or as late as 10 weeks of age
• substantial neutrophilic infiltration with slight to severe bone and cartilage erosion is seen in the tibiotarsal joint
• mice treated with an alpha IL-1R blocking antibody show decreased tibiotarsal joint swelling
• substantial neutrophilic infiltration with slight to severe bone and cartilage erosion is seen in the tibiotarsal joint

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
primary immunodeficiency disease DOID:612 OMIM:242850
OMIM:PS300755
J:260047




Genotype
MGI:6196855
cn3
Allelic
Composition
Gt(ROSA)26Sortm1(OVAL/fla,GFP)Vnce/Gt(ROSA)26Sor+
Lyz2tm1(cre)Ifo/Lyz2+
Nlrc4tm1Vmd/Nlrc4tm1Vmd
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6J * C57BL/6NCrl
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1(OVAL/fla,GFP)Vnce mutation (0 available); any Gt(ROSA)26Sor mutation (758 available)
Lyz2tm1(cre)Ifo mutation (13 available); any Lyz2 mutation (28 available)
Nlrc4tm1Vmd mutation (1 available); any Nlrc4 mutation (46 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
N
• neutrophilic inflammation is not apparent

skeleton
N
• mice exhibit normal joints




Genotype
MGI:6196860
cn4
Allelic
Composition
Gt(ROSA)26Sortm1(OVAL/fla,GFP)Vnce/Gt(ROSA)26Sor+
Tg(S100A8-cre,-EGFP)1Ilw/0
Genetic
Background
involves: C3H * C57BL/6 * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1(OVAL/fla,GFP)Vnce mutation (0 available); any Gt(ROSA)26Sor mutation (758 available)
Tg(S100A8-cre,-EGFP)1Ilw mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
growth/size/body
• mice are runted
• mice treated with an alpha IL-1R blocking antibody show weight gain

hematopoietic system
• increase in neutrophils in the spleen and lymph nodes
• increase in monocytes in the spleen and lymph nodes

homeostasis/metabolism
• mice exhibit increased cytokine levels, with IL-18 having the greatest increase
• mice develop joint swelling
• mice treated with an alpha IL-1R blocking antibody show deceased joint swelling

immune system
• increase in neutrophils in the spleen and lymph nodes
• increase in monocytes in the spleen and lymph nodes
• mice exhibit increased cytokine levels, with IL-18 having the greatest increase

skeleton
• mice develop joint swelling
• mice treated with an alpha IL-1R blocking antibody show deceased joint swelling




Genotype
MGI:6196862
cn5
Allelic
Composition
Gt(ROSA)26Sortm1(OVAL/fla,GFP)Vnce/Gt(ROSA)26Sor+
Tg(Itgax-cre)1-1Reiz/0
Genetic
Background
involves: C3H * C57BL/6 * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1(OVAL/fla,GFP)Vnce mutation (0 available); any Gt(ROSA)26Sor mutation (758 available)
Tg(Itgax-cre)1-1Reiz mutation (3 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
growth/size/body
• mice are runted

hematopoietic system
• mice exhibit elevation of neutrophils in tissues
• mice exhibit elevation of monocytes in tissues

immune system
• mice exhibit elevation of neutrophils in tissues
• mice exhibit elevation of monocytes in tissues

skeleton
N
• mice do not develop joint swellings





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last database update
05/10/2022
MGI 6.19
The Jackson Laboratory