Phenotypes associated with this allele

• Allele Symbol: Gimap6^tm1.1Gwb
• Allele Name: targeted mutation 1.1, Geoffrey W Butcher
• Allele ID: MGI:6160453
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gimap6^tm1.1Gwb/Gimap6^tm1.1Gwb Tg(CD2-icre)4Kio/0	involves: C57BL/6 * C57BL/10 * CBA/Ca	MGI:6160464

Genotype
MGI:6160464

cn1

• Allelic Composition: Gimap6^tm1.1Gwb/Gimap6^tm1.1Gwb; Tg(CD2-icre)4Kio/0
• Genetic Background: involves: C57BL/6 * C57BL/10 * CBA/Ca

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

abnormal mitochondrial morphology ( J:262059 )

• occasional mitochondrial spheroid observed in Cd4+ T splenocytes by electron microscopy

• significant increase in mitochondrial/cytoplasmic volume ratio in Cd4+ T splenocytes as indicated by electron microscopy

abnormal autophagy ( J:262059 )

• increased levels of Map1lc3b (particularly the LC3-II variant) protein in Cd4+ T cells and Cd8+ T and B cells isolated from spleen, indicating increased number of autophagosomes

• increased number of autophagosomes per Cd4+ T splenocyte as indicated by electron microscopy

abnormal mitophagy ( J:262059 )

• significant increase in mitochondrial/cytoplasmic volume ratio in Cd4+ T splenocytes as indicated by electron microscopy

increased T cell apoptosis ( J:262059 )

• of Cd4+ T splenocytes as indicated by annexin V+ DAPI- staining

• younger age profile of Cd4+ T splenocytes as indicated by Cd24+ staining

hematopoietic system

abnormal leukocyte morphology ( J:262059 )

• increase in cytoplasmic surface area per nucleated Cd4+ T splenocyte as indicated by electron microscopy

• increase in Cd4+ T cells and Cd8+ T splenocyte diameter

decreased B cell number ( J:262059 )

• significant reduction in recirculating B cell percentage in bone marrow

increased B cell number ( J:262059 )

• significant increase in percentages and numbers of follicular, marginal zone, T1, T2 and T3 B splenocytes

• significant increase in percentage, but not number, of mature B cells in lymph nodes

decreased T cell number ( J:262059 )

• 50-70% reduction in percentage and number of Cd4+ and Cd8+ T cells in spleen and lymph nodes

• Cd4- Cd8-, Cd4+ Cd8+, Cd4+ and Cd8+ T cell percentages in thymus

abnormal T cell physiology ( J:262059 )

• shift from Cd62l(high) Cd44(low) to Cd62l(low) Cd44(high) cell surface expression on splenic and lymph node Cd4+ T cells

• reduction in Cd44(high) fraction of splenic and lymph node Cd8+ T cells

• Cd69 surface expression on Cd4+ and Cd8+ T splenocytes

increased T cell apoptosis ( J:262059 )

• of Cd4+ T splenocytes as indicated by annexin V+ DAPI- staining

• younger age profile of Cd4+ T splenocytes as indicated by Cd24+ staining

immune system

abnormal leukocyte morphology ( J:262059 )

• increase in cytoplasmic surface area per nucleated Cd4+ T splenocyte as indicated by electron microscopy

• increase in Cd4+ T cells and Cd8+ T splenocyte diameter

decreased B cell number ( J:262059 )

• significant reduction in recirculating B cell percentage in bone marrow

increased B cell number ( J:262059 )

• significant increase in percentages and numbers of follicular, marginal zone, T1, T2 and T3 B splenocytes

• significant increase in percentage, but not number, of mature B cells in lymph nodes

decreased T cell number ( J:262059 )

• 50-70% reduction in percentage and number of Cd4+ and Cd8+ T cells in spleen and lymph nodes

• Cd4- Cd8-, Cd4+ Cd8+, Cd4+ and Cd8+ T cell percentages in thymus

abnormal T cell physiology ( J:262059 )

• shift from Cd62l(high) Cd44(low) to Cd62l(low) Cd44(high) cell surface expression on splenic and lymph node Cd4+ T cells

• reduction in Cd44(high) fraction of splenic and lymph node Cd8+ T cells

• Cd69 surface expression on Cd4+ and Cd8+ T splenocytes

increased T cell apoptosis ( J:262059 )

• of Cd4+ T splenocytes as indicated by annexin V+ DAPI- staining

• younger age profile of Cd4+ T splenocytes as indicated by Cd24+ staining

homeostasis/metabolism

abnormal autophagy ( J:262059 )

• increased levels of Map1lc3b (particularly the LC3-II variant) protein in Cd4+ T cells and Cd8+ T and B cells isolated from spleen, indicating increased number of autophagosomes

• increased number of autophagosomes per Cd4+ T splenocyte as indicated by electron microscopy

abnormal mitophagy ( J:262059 )

• significant increase in mitochondrial/cytoplasmic volume ratio in Cd4+ T splenocytes as indicated by electron microscopy