Phenotypes associated with this allele

• Allele Symbol: Nalcn^{tm1c(KOMP)Wtsi}
• Allele Name: targeted mutation 1c, Wellcome Trust Sanger Institute
• Allele ID: MGI:5823777
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Nalcn^tm1c(KOMP)Wtsi/Nalcn^tm1c(KOMP)Wtsi Tg(Camk2a-cre)2Gsc/0	involves: C57BL/6N * FVB/N	MGI:5823855

Genotype
MGI:5823855

cn1

• Allelic Composition: Nalcn^{tm1c(KOMP)Wtsi}/Nalcn^{tm1c(KOMP)Wtsi}; Tg(Camk2a-cre)2Gsc/0
• Genetic Background: involves: C57BL/6N * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

lethality at weaning, complete penetrance ( J:238767 )

• mice die at ~21 days of age

nervous system

abnormal nervous system electrophysiology ( J:238767 )

• N-methyl-D-glucamine (NMDG)-evoked hyperpolarization is greatly reduced relative to that in age-matched sibling controls

• in the presence of tetrodotoxin (a voltage-dependent sodium channel blocker) and potassium (K) blockers, the sodium leak current flowing during the interspike interval (I_NALCN) is reduced in organotypic slices containing SCN after replacement of extracellular sodium with NMDG

impaired ability to fire action potentials ( J:238767 )

• suprachiasmatic nucleus (SCN) neurons are hyperpolarized and silent, consistent with a role of NALCN in controlling the membrane potential and firing frequency of neurons

• a small depolarization current injected into SCN neurons is able to evoke strong firing, indicating that cells are healthy but require a greater depolarizing input to evoke action potentials