Phenotypes associated with this allele

• Allele Symbol: Dpp4^{tm1(DPP4)Vlcg}
• Allele Name: targeted mutation 1, Velocigene
• Allele ID: MGI:5788094
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ot1	Dpp4^tm1(DPP4)Vlcg/?	involves: 129S6/SvEvTac * C57BL/6 * C57BL/6NTac	MGI:6435210
ot2	Dpp4^tm1(DPP4)Vlcg/?	involves: 129S6/SvEvTac * C57BL/6NTac	MGI:6400455

Genotype
MGI:6435210

ot1

• Allelic Composition: Dpp4^{tm1(DPP4)Vlcg}/?
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * C57BL/6NTac

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

increased neutrophil cell number ( J:238079 )

• lungs of mice infected with a high dose of MERS-CoV are infiltrated with neutrophils; neutrophils infiltrate up to 4 dpi then return to normal levels

increased CD4-positive, alpha-beta T cell number ( J:238079 )

• lungs of mice infected with a high dose of MERS-CoV are infiltrated with CD4+ T cells over the course of infection; CD4+ T cells infiltrate after 4 dpi and reach high levels by 7 dpi

increased CD8-positive, alpha-beta T cell number ( J:238079 )

• lungs of mice infected with a high dose of MERS-CoV are infiltrated with CD8+ T cells over the course of infection; CD8+T cells infiltrate after 4 dpi and reach high levels by 7 dpi

increased macrophage cell number ( J:238079 )

• lungs of mice infected with a high dose of MERS-CoV are infiltrated with macrophages over the course of infection; macrophages infiltrate after 4 dpi and remain at high levels by 7 dpi

lung inflammation ( J:238079 )

• mice infected with MERS-CoV show lymphocytic perivascular inflammation, macrophage infiltration, and pleuritis

• mice infected with the intermediate dose of MERS-CoV resolve all signs of disease including lymphocytic perivascular inflammation by 14 dpi while mice infected with the low dose still show lymphocytic perivascular inflammation through 21 dpi but resolve all other inflammatory parameters by 14 dpi

• lungs of mice infected with a high dose of MERS-CoV are infiltrated with macrophages, neutrophils, and CD4+ and CD8+ T cells over the course of infection

pleuritis ( J:238079 )

• mice infected with MERS-CoV show pleuritis

increased susceptibility to Coronaviridae infection ( J:238079 )

• mice infected with a high dose of MERS-CoV-Hu/Jordan-N3/2012 intranasally lose more than 20% of weight by 7 days postinfection (dpi) and show progressively worsening signs of disease such as ruffled fur, lethargy, and hunched bearing

• mice infected with an intermediate dose of MERS-CoV intranasally lose 10% of body weight by 7 dpi but regain 5% of starting body weight by 8 dpi and remain at 100% of starting body weight from 9 dpi and show no other signs of disease

• mice infected with a low dose of MERS-CoV intranasally do not lose weight and show no signs of disease

• mice infected with the high dose of MERS-CoV show viral RNA in the lungs, blood and spleen, but not in the brain, liver, or kidney

respiratory system

pulmonary epithelial necrosis ( J:238079 )

• mice infected with MERS-CoV Jordan show epithelial necrosis

pulmonary edema ( J:238079 )

• mice infected with the high dose of MERS-CoV show moderate lung edema by 7 dpi

lung inflammation ( J:238079 )

• mice infected with MERS-CoV show lymphocytic perivascular inflammation, macrophage infiltration, and pleuritis

• mice infected with the intermediate dose of MERS-CoV resolve all signs of disease including lymphocytic perivascular inflammation by 14 dpi while mice infected with the low dose still show lymphocytic perivascular inflammation through 21 dpi but resolve all other inflammatory parameters by 14 dpi

• lungs of mice infected with a high dose of MERS-CoV are infiltrated with macrophages, neutrophils, and CD4+ and CD8+ T cells over the course of infection

pleuritis ( J:238079 )

• mice infected with MERS-CoV show pleuritis

homeostasis/metabolism

pulmonary edema ( J:238079 )

• mice infected with the high dose of MERS-CoV show moderate lung edema by 7 dpi

hematopoietic system

increased neutrophil cell number ( J:238079 )

• lungs of mice infected with a high dose of MERS-CoV are infiltrated with neutrophils; neutrophils infiltrate up to 4 dpi then return to normal levels

increased CD4-positive, alpha-beta T cell number ( J:238079 )

• lungs of mice infected with a high dose of MERS-CoV are infiltrated with CD4+ T cells over the course of infection; CD4+ T cells infiltrate after 4 dpi and reach high levels by 7 dpi

increased CD8-positive, alpha-beta T cell number ( J:238079 )

• lungs of mice infected with a high dose of MERS-CoV are infiltrated with CD8+ T cells over the course of infection; CD8+T cells infiltrate after 4 dpi and reach high levels by 7 dpi

increased macrophage cell number ( J:238079 )

• lungs of mice infected with a high dose of MERS-CoV are infiltrated with macrophages over the course of infection; macrophages infiltrate after 4 dpi and remain at high levels by 7 dpi

cellular

pulmonary epithelial necrosis ( J:238079 )

• mice infected with MERS-CoV Jordan show epithelial necrosis

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
Middle East respiratory syndrome		DOID:0080642		J:238079

Genotype
MGI:6400455

ot2

• Allelic Composition: Dpp4^{tm1(DPP4)Vlcg}/?
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6NTac

immune system

lung inflammation ( J:223728 )

• mice infected with MERS-CoV intranasally show peribronchiolar inflammation at 2 days post infection

interstitial pneumonia ( J:223728 )

• mice infected with MERS-CoV intranasally show lung pathology consistent with development of interstitial pneumonia and lung disease

increased susceptibility to Coronaviridae infection ( J:223728 )

• mice support efficient infection and replication of the human Middle East respiratory syndrome coronavirus (MERS-CoV) , with mice developing peribronchiolar inflammation and lung pathology including peri-vascular cuffing and extensive alveolar thickening, and consistent with development of interstitial pneumonia and lung disease

• however, no viral RNA or inflammation is seen in the brain of MERS-CoV infected mice

• mice pretreated with REGN3051 or REGN3048, antibodies which bind to the MERS-CoV receptor-binding domain, show decreased viral levels and lung pathology after infection with MERS-CoV

• mice infected with MERS-CoV and then treated with REGN3051 at 24 hours after infection, show decreased viral levels and lung pathology

respiratory system

lung inflammation ( J:223728 )

• mice infected with MERS-CoV intranasally show peribronchiolar inflammation at 2 days post infection

interstitial pneumonia ( J:223728 )

• mice infected with MERS-CoV intranasally show lung pathology consistent with development of interstitial pneumonia and lung disease

thick pulmonary interalveolar septum ( J:223728 )

• mice infected with MERS-CoV intranasally develop extensive alveolar septum thickening at 4 dpi

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
Middle East respiratory syndrome		DOID:0080642		J:223728