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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Tg(Mup3-Plau)350-2Eps
transgene insertion 350-2, Eric P Sandgren
MGI:5781013
Summary 4 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Ddit3tm1.1Irt/Ddit3tm1.1Irt
Speer6-ps1Tg(Alb-cre)21Mgn/Speer6-ps1+
Tg(Mup3-Plau)350-2Eps/?
involves: C57BL/6 * DBA MGI:5781097
tg2
Tg(Mup3-Plau)350-2Eps/0 involves: C57BL/6 MGI:5781014
tg3
Tg(Mup3-Plau)350-2Eps/0 involves: C57BL/6 * FVB/N MGI:5781015
tg4
Tg(Mup3-Plau)350-2Eps/? C57BL/6-Tg(Mup3-Plau)350-2Eps MGI:5781017


Genotype
MGI:5781097
cn1
Allelic
Composition
Ddit3tm1.1Irt/Ddit3tm1.1Irt
Speer6-ps1Tg(Alb-cre)21Mgn/Speer6-ps1+
Tg(Mup3-Plau)350-2Eps/?
Genetic
Background
involves: C57BL/6 * DBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ddit3tm1.1Irt mutation (1 available); any Ddit3 mutation (16 available)
Speer6-ps1Tg(Alb-cre)21Mgn mutation (6 available); any Speer6-ps1 mutation (4 available)
Tg(Mup3-Plau)350-2Eps mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
liver/biliary system
• mice fed a high fat diet develop hepatocellular carcinoma and show increased tumor multiplicity without affecting tumor size compared to single Tg(Mup3-Plau)350-2Eps transgenic mice

neoplasm
• mice fed a high fat diet develop hepatocellular carcinoma and show increased tumor multiplicity without affecting tumor size compared to single Tg(Mup3-Plau)350-2Eps transgenic mice




Genotype
MGI:5781014
tg2
Allelic
Composition
Tg(Mup3-Plau)350-2Eps/0
Genetic
Background
involves: C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
No mouse lines available in IMSR.
See publication links below for author information.
phenotype observed in females
phenotype observed in males
N normal phenotype
homeostasis/metabolism
• serum alanine aminotransferase activity begins to increase at 4 weeks of age, peaks at 5 weeks, and returns to normal by 13 weeks of age
• however, mice exhibit normal serum total protein and albumin concentrations

liver/biliary system
• livers become slightly pale between 3 and 4 week of age
• mice develop diffuse lesions in the livers by 1 month of age
• multiple small red foci become visible on the surface of the liver between 4 and 5 weeks of age
• by 8 weeks of age, livers are entirely red but have a rough, nodular surface
• older mice develop hepatic neoplasms
• hepatic tumors show a latency of 9 to 26 months

neoplasm
• mice develop diffuse lesions in the livers by 1 month of age
• multiple small red foci become visible on the surface of the liver between 4 and 5 weeks of age
• by 8 weeks of age, livers are entirely red but have a rough, nodular surface
• older mice develop hepatic neoplasms
• hepatic tumors show a latency of 9 to 26 months

cardiovascular system
N
• mice do not exhibit perinatal hemorrhage




Genotype
MGI:5781015
tg3
Allelic
Composition
Tg(Mup3-Plau)350-2Eps/0
Genetic
Background
involves: C57BL/6 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
No mouse lines available in IMSR.
See publication links below for author information.
phenotype observed in females
phenotype observed in males
N normal phenotype
integument
• some mice display subcutaneous edema

homeostasis/metabolism
• some mice display subcutaneous edema




Genotype
MGI:5781017
tg4
Allelic
Composition
Tg(Mup3-Plau)350-2Eps/?
Genetic
Background
C57BL/6-Tg(Mup3-Plau)350-2Eps
Find Mice Using the International Mouse Strain Resource (IMSR)
No mouse lines available in IMSR.
See publication links below for author information.
phenotype observed in females
phenotype observed in males
N normal phenotype
growth/size/body
• 30% of normal chow fed mice display a few tiny nodules in the liver at 40 weeks of age, indicating hyperplasia

cellular
• high fat diet fed mice show distended and dilated endoplasmic reticulum
• livers of high fat diet fed mice exhibit continuous hepatocyte death and compensatory proliferation
• hepatocytes treated with PA show more extensive lipotoxic cell death than wild-type hepatocytes
• PA treated hepatocytes treated with the chemical chaperons 4-phenylbutyrate (4-PBA) and tauro-ursodeoxycholic acid (TUDCA), which reduce ER stress, show a more pronounced attenuation of cell death than wild-type hepatocytes
• livers of high fat diet fed mice show increased apoptotic and necrotic cell death

homeostasis/metabolism
• mice fed a high fat diet starting at 6 weeks of age maintain high serum alanine aminotransferase (ALT) levels throughout the observation period
• high fat diet fed mice treated with TUDCA at 16 weeks of age exhibit reduced serum ALT levels
• mice fed a high fat diet exhibit elevated liver cholesterol levels
• high fat diet fed mice treated with TUDCA at 16 weeks of age exhibit reduced hepatic cholesterol levels
• mice fed a standard chow diet show an increase in C16:0 palmitic acid and longer chain fatty acid in the liver compared to wild-type mice, which is further enhanced by high fat diet feeding
• mice fed a high fat diet exhibit elevated liver triglycerides
• high fat diet fed mice treated with TUDCA at 16 weeks of age exhibit hepatic triglyceride levels

immune system
• high fat diet fed mice show increased levels of IL-1beta in the liver at 24 weeks of age
• high fat diet fed mice show increased levels of TNF in the liver at 24 weeks of age
• TUDCA treatment of high fat diet fed mice inhibits the TNF increase seen in the liver
• mice fed a high fat diet exhibit extensive immune infiltration into the liver and numerous ballooning hepatocytes, indicating non-alcoholic steatohepatitis
• mice fed a high fat diet show an increase in the number of F4/80-positive macrophages in the liver
• TUDCA treatment of high fat diet fed mice inhibits the increase in macrophage infiltration in the liver

liver/biliary system
• livers of high fat diet fed mice exhibit continuous hepatocyte death and compensatory proliferation
• hepatocytes treated with PA show more extensive lipotoxic cell death than wild-type hepatocytes
• PA treated hepatocytes treated with the chemical chaperons 4-phenylbutyrate (4-PBA) and tauro-ursodeoxycholic acid (TUDCA), which reduce ER stress, show a more pronounced attenuation of cell death than wild-type hepatocytes
• livers of high fat diet fed mice show increased apoptotic and necrotic cell death
• 30% of normal chow fed mice display a few tiny nodules in the liver at 40 weeks of age, indicating hyperplasia
• mice fed a high fat diet exhibit elevated liver cholesterol levels
• high fat diet fed mice treated with TUDCA at 16 weeks of age exhibit reduced hepatic cholesterol levels
• mice fed a standard chow diet show an increase in C16:0 palmitic acid and longer chain fatty acid in the liver compared to wild-type mice, which is further enhanced by high fat diet feeding
• mice fed a high fat diet exhibit elevated liver triglycerides
• high fat diet fed mice treated with TUDCA at 16 weeks of age exhibit hepatic triglyceride levels
• mice fed a high fat diet exhibit extensive immune infiltration into the liver and numerous ballooning hepatocytes, indicating non-alcoholic steatohepatitis
• mice fed a high fat diet show an increase in the number of F4/80-positive macrophages in the liver
• TUDCA treatment of high fat diet fed mice inhibits the increase in macrophage infiltration in the liver
• mice fed normal chow exhibit hepatocyte damage at 5 weeks of age but this disappears at 24 weeks, except for mild inflammation and spotty necrosis
• mice fed a high fat diet exhibit numerous ballooning hepatocytes
• high fat diet fed mice treated with TUDCA at 16 weeks of age exhibit attenuation of hepatocyte ballooning
• mice fed a standard chow diet exhibit mild spontaneous lipid accumulation in the liver at 5 weeks of age that is diminished by 16 weeks of age
• mice fed a high fat diet show extensive lipid accumulation in the liver
• high fat diet fed mice treated with TUDCA at 16 weeks of age exhibit attenuation of hepatosteatosis
• mice fed a high fat diet show pericelluar and bridging fibrosis in the liver
• high fat diet fed mice develop small tumors on the liver surface by 32 weeks of age and large tumors at 40 weeks
• 30% of tumors larger than 2 mm are hepatocellular carcinomas, similar to human steatohepatitic hepatocellular carcinomas, although some show a classical thick trabecular pattern
• hepatocellular carcinoma progenitor cell-transplanted mutants develop multiple hepatocellular carcinoma nodules after 5 months
• 70% of tumors are either typical or steatohepatic adenomas

neoplasm
• high fat diet fed mice develop small tumors on the liver surface by 32 weeks of age and large tumors at 40 weeks
• 30% of tumors larger than 2 mm are hepatocellular carcinomas, similar to human steatohepatitic hepatocellular carcinomas, although some show a classical thick trabecular pattern
• hepatocellular carcinoma progenitor cell-transplanted mutants develop multiple hepatocellular carcinoma nodules after 5 months
• 70% of tumors are either typical or steatohepatic adenomas





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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
05/17/2022
MGI 6.19
The Jackson Laboratory