Phenotypes associated with this allele

• Allele Symbol: Gt(ROSA)26Sor^tm1(Fgf8)Lma
• Allele Name: targeted mutation 1, Liang Ma
• Allele ID: MGI:5694148
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Ctnnb1^tm2Kem/Ctnnb1^tm2Kem Gt(ROSA)26Sor^tm1(Fgf8)Lma/Gt(ROSA)26Sor^+ Shh^tm1(EGFP/cre)Cjt/Shh^+	involves: 129S1/Sv * 129X1/SvJ	MGI:5694211
cn2	Ctnnb1^tm2Kem/Ctnnb1^tm2Kem Gt(ROSA)26Sor^tm1(Fgf8)Lma/Gt(ROSA)26Sor^+ Tg(Msx2-cre)5Rem/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA	MGI:5694212
cn3	Gt(ROSA)26Sor^tm1(Fgf8)Lma/Gt(ROSA)26Sor^+ Shh^tm2(cre/ERT2)Cjt/Shh^+	involves: 129S6/SvEvTac	MGI:5694209
cn4	Gt(ROSA)26Sor^tm1(Fgf8)Lma/Gt(ROSA)26Sor^+ Shh^tm2(cre/ERT2)Cjt/Shh^tm2(cre/ERT2)Cjt	involves: 129S6/SvEvTac	MGI:5694214
cn5	Gt(ROSA)26Sor^tm1(Fgf8)Lma/Gt(ROSA)26Sor^+ Shh^tm2(cre/ERT2)Cjt/Shh^tm2(cre/ERT2)Cjt Tg(Msx2-cre)5Rem/0	involves: 129S6/SvEvTac * C57BL/6 * CBA	MGI:5694215
cn6	Gt(ROSA)26Sor^tm1(Fgf8)Lma/Gt(ROSA)26Sor^+ Tg(Msx2-cre)5Rem/0	involves: C57BL/6 * CBA	MGI:5694210

Genotype
MGI:5694211

cn1

• Allelic Composition: Ctnnb1^tm2Kem/Ctnnb1^tm2Kem; Gt(ROSA)26Sor^tm1(Fgf8)Lma/Gt(ROSA)26Sor⁺; Shh^{tm1(EGFP/cre)Cjt}/Shh⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

reproductive system phenotype ( J:223057 )

N • a cone-shaped tubercle structure is discernable unlike in urethral epithelium knock-out of beta-catenin

abnormal reproductive system development ( J:223057 )

• mice exhibit failed cloaca septation

limbs/digits/tail

abnormal tail development ( J:223057 )

Genotype
MGI:5694212

cn2

• Allelic Composition: Ctnnb1^tm2Kem/Ctnnb1^tm2Kem; Gt(ROSA)26Sor^tm1(Fgf8)Lma/Gt(ROSA)26Sor⁺; Tg(Msx2-cre)5Rem/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA

limbs/digits/tail

limbs/digits/tail phenotype ( J:223057 )

N • unlike in mice lacking Fgf8 over-expression, mice develop normal humeri with deltoid tuberosity and the radius is evident

abnormal autopod morphology ( J:223057 )

• autopod rudiments

abnormal ulna morphology ( J:223057 )

• the ulna is longer and thicker

abnormal hindlimb morphology ( J:223057 )

• near normal pelvic girdles and femurs with one or two ectopic cartilages

skeleton

abnormal ulna morphology ( J:223057 )

• the ulna is longer and thicker

Genotype
MGI:5694209

cn3

• Allelic Composition: Gt(ROSA)26Sor^tm1(Fgf8)Lma/Gt(ROSA)26Sor⁺; Shh^{tm2(cre/ERT2)Cjt}/Shh⁺
• Genetic Background: involves: 129S6/SvEvTac

reproductive system

abnormal genital tubercle morphology ( J:223057 )

• larger than in controls

abnormal reproductive system physiology ( J:223057 )

• mice treated with tamoxifen at E10.5 exhibit increased mitotic index in mesoderm-derived para-cloacal mesenchyme compared with wild-type mice

Genotype
MGI:5694214

cn4

• Allelic Composition: Gt(ROSA)26Sor^tm1(Fgf8)Lma/Gt(ROSA)26Sor⁺; Shh^{tm2(cre/ERT2)Cjt}/Shh^{tm2(cre/ERT2)Cjt}
• Genetic Background: involves: 129S6/SvEvTac

reproductive system

abnormal genital tubercle morphology ( J:223057 )

• after 48 hrs of tamoxifen treatment, no tubercle formation is observed at E12.5

Genotype
MGI:5694215

cn5

• Allelic Composition: Gt(ROSA)26Sor^tm1(Fgf8)Lma/Gt(ROSA)26Sor⁺; Shh^{tm2(cre/ERT2)Cjt}/Shh^{tm2(cre/ERT2)Cjt}; Tg(Msx2-cre)5Rem/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * CBA

limbs/digits/tail

abnormal limb development ( J:223057 )

• as in Gt(ROSA)26Sor^tm1Lma/Gt(ROSA)26Sor⁺ Shh^{tm2(cre/ERT2)Cjt}/Shh^{tm2(cre/ERT2)Cjt} mice

Genotype
MGI:5694210

cn6

• Allelic Composition: Gt(ROSA)26Sor^tm1(Fgf8)Lma/Gt(ROSA)26Sor⁺; Tg(Msx2-cre)5Rem/0
• Genetic Background: involves: C57BL/6 * CBA

limbs/digits/tail

polydactyly ( J:223057 )

abnormal calcaneum morphology ( J:223057 )

• enlarged

abnormal limb development ( J:223057 )

• excessive limb growth

skeleton

abnormal calcaneum morphology ( J:223057 )

• enlarged

abnormal skeleton development ( J:223057 )

• ectopic skeletal elements in the fore- and hindlimbs