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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Gt(ROSA)26Sortm1(CAG-cas9*,-EGFP)Fezh
targeted mutation 1, Feng Zhang
MGI:5583839
Summary 2 genotypes


Genotype
MGI:5583861
hm1
Allelic
Composition
Gt(ROSA)26Sortm1(CAG-cas9*,-EGFP)Fezh/Gt(ROSA)26Sortm1(CAG-cas9*,-EGFP)Fezh
Genetic
Background
B6;129-Gt(ROSA)26Sortm1(CAG-cas9*,-EGFP)Fezh
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1(CAG-cas9*,-EGFP)Fezh mutation (5 available); any Gt(ROSA)26Sor mutation (745 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
normal phenotype
• mice are viable and fertile




Genotype
MGI:5897845
cn2
Allelic
Composition
Gt(ROSA)26Sortm1(CAG-cas9*,-EGFP)Fezh/?
Tmem258tm1.1(KOMP)Vlcg/Tmem258+
Genetic
Background
involves: C57BL/6NTac
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1(CAG-cas9*,-EGFP)Fezh mutation (5 available); any Gt(ROSA)26Sor mutation (745 available)
Tmem258tm1.1(KOMP)Vlcg mutation (0 available); any Tmem258 mutation (10 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• after introduction of single guide RNAs (sgRNAs) targeting exonic Tmem258 sequences (Tmem258 CRISPR) by transduction with lentiviral particles co-encoding Cre recombinase, spheroid cultures derived from colonic crypts exhibit fewer viable Cas9-GFP-positive cells than colonic organoid cultures that have received control sgRNAs targeting noncoding intronic Tmem258 regions
• morphologically, Tmem258 CRISPR organoids contain scattered Cas9-GFP-positive cells within small spheroids that encapsulate numerous dead cells within the lumen, whereas control CRISPR organoids appear healthier and larger with contiguous Cas9-GFP-positive cells suggestive of active proliferation
• after simultaneous delivery of Cre recombinase and sgRNA targeting of Tmem258 sequences by lentiviral transduction, Tmem258 CRISPR organoid cultures contain more in-frame mutations in Cas9-GFP-positive cells than control CRISPR organoids, suggesting that out-of-frame alleles are negatively selected, consistent with increased ER stress





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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
01/04/2022
MGI 6.17
The Jackson Laboratory