Phenotypes associated with this allele

• Allele Symbol: Gnl3^tm2.1Rylt
• Allele Name: targeted mutation 2.1, Robert Y L Tsai
• Allele ID: MGI:5523416
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gnl3^tm2.1Rylt/Gnl3^tm2.1Rylt Tg(CAG-cre/Esr1*)5Amc/0	involves: 129 * 129S2/SvPas * C57BL/6 * CBA	MGI:5523423
cn2	Gnl3^tm2.1Rylt/Gnl3^tm2.1Rylt Trp53^tm1Tyj/Trp53^tm1Tyj Tg(CAG-cre/Esr1*)5Amc/0	involves: 129 * 129S2/SvPas * C57BL/6 * CBA	MGI:5523425
cn3	Gnl3^tm2.1Rylt/Gnl3^tm2.1Rylt Tg(Nes-cre)1Kln/0	involves: 129 * C57BL/6 * CBA	MGI:5523421

Genotype
MGI:5523423

cn1

• Allelic Composition: Gnl3^tm2.1Rylt/Gnl3^tm2.1Rylt; Tg(CAG-cre/Esr1*)5Amc/0
• Genetic Background: involves: 129 * 129S2/SvPas * C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

abnormal cell physiology ( J:198698 )

• increase in the percentage of MEFs showing signs of replication-induced DNA damage after tamoxifen treatment

decreased fibroblast proliferation ( J:198698 )

• tamoxifen treatment impairs the long-term proliferative potential of MEFs

Genotype
MGI:5523425

cn2

• Allelic Composition: Gnl3^tm2.1Rylt/Gnl3^tm2.1Rylt; Trp53^tm1Tyj/Trp53^tm1Tyj; Tg(CAG-cre/Esr1*)5Amc/0
• Genetic Background: involves: 129 * 129S2/SvPas * C57BL/6 * CBA

cellular

abnormal cell physiology ( J:198698 )

• increase in the percentage of MEFs showing signs of replication-induced DNA damage after tamoxifen treatment

abnormal cell death ( J:198698 )

• tamoxifen treatment shortens the lifespan of MEFs

decreased fibroblast proliferation ( J:198698 )

• tamoxifen treatment impairs the long-term proliferative potential of MEFs

Genotype
MGI:5523421

cn3

• Allelic Composition: Gnl3^tm2.1Rylt/Gnl3^tm2.1Rylt; Tg(Nes-cre)1Kln/0
• Genetic Background: involves: 129 * C57BL/6 * CBA

Severe brain defects in Gnl3^tm2.1Rylt/Gnl3^tm2.1Rylt Tg(Nes-cre)1Kln/0 embryos

mortality/aging

neonatal lethality, complete penetrance ( J:198698 )

respiratory system

respiratory failure ( J:198698 )

nervous system

abnormal embryonic neuroepithelium morphology ( J:198698 )

• decrease in cellularity starting at E12.5

• increase in gamma-H2AFX protein in cells expressing this protein at E12.5

abnormal brain morphology ( J:198698 )

abnormal cerebellum development ( J:198698 )

• major defects in the cerebellar primordium at E14.5 and E16.5

abnormal tectum morphology ( J:198698 )

• major defects at E14.5 and E16.5

decreased tegmentum size ( J:198698 )

• smaller midbrain and hindbrain tegmentum at E14.5 and E16.5

abnormal diencephalon morphology ( J:198698 )

• smaller at E14.5 and E16.5

abnormal telencephalon morphology ( J:198698 )

• major defects in the cortex at E14.5 and E16.5

abnormal embryonic/fetal subventricular zone morphology ( J:198698 )

• major defects in the ganglionic eminence at E14.5 and E16.5

abnormal neuronal stem cell morphology ( J:198698 )

• decrease in neuronal stem cell numbers in the neuroepithelium at E12.5

decreased spinal cord size ( J:198698 )

• at E14.5 and E16.5

embryo

abnormal embryonic neuroepithelium morphology ( J:198698 )

• decrease in cellularity starting at E12.5

• increase in gamma-H2AFX protein in cells expressing this protein at E12.5