• tumors have higher grades of malignancy and are less differentiated than those of single Tg(Pklr-Myc)73Ak mice

• mutants develop hepatocellular carcinoma

• the number and size of tumor nodules in double mutants is greater than in single Tg(Pklr-Myc)73Ak mice

• some tumors in double mutants exhibit a very poorly differentiated phenotype (fetal-like phenotype) that is never seen in tumors of single Tg(Pklr-Myc)73Ak mice

• tumors have higher mitotic indices compared with tumors from single Tg(Pklr-Myc)73Ak mice

• 40% of double mutants exhibit lung metastasis which is not seen in single Tg(Pklr-Myc)73Ak mice

• tumors have higher grades of malignancy and are less differentiated than those of single Tg(Pklr-Myc)73Ak mice

• mutants develop hepatocellular carcinoma (HCC); stronger promotion of initiation and progression steps of HCC development compared to single Tg(Pklr-Myc)73Ak mice

• the number and size of tumor nodules in double mutants is greater than in single Tg(Pklr-Myc)73Ak mice

• some tumors in double mutants exhibit a very poorly differentiated phenotype (fetal-like phenotype) that is never seen in tumors of single Tg(Pklr-Myc)73Ak mice

• tumors have higher mitotic indices compared with tumors from single Tg(Pklr-Myc)73Ak mice

• the proportion of invariant NKT cells in the liver is lower than in controls

• the proportion of invariant NKT cells in the liver is lower than in controls

• mutants fed a carbohydrate-rich diet develop hepatocarcinomas

• frequency of hepatocarcinomas is positively correlated with the carbohydrate content of the diet; frequency increases from 14.3% to 31.7% and 65.2% when the carbohydrate ratio is increased from 10% to 50% to 75%, respectively

• mutants fed a carbohydrate-rich diet frequently develop hepatomas

• mutants fed a carbohydrate-rich diet exhibit pancreatic islet hyperplasia, however they do not develop pancreatic tumors