Phenotypes associated with this allele

• Allele Symbol: Panx1^tm1.1Fama
• Allele Name: targeted mutation 1.1, Fabio Mammano
• Allele ID: MGI:5297171
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Panx1^tm1.1Fama/Panx1^tm1.1Fama	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5297176
cx2	Panx1^tm1.1Fama/Panx1^tm1.1Fama Panx2^tm1.1Mony/Panx2^tm1.1Mony	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5311017
cx3	Panx1^tm1.1Fama/Panx1^+ Panx2^tm1.1Mony/Panx2^+	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5311018

Genotype
MGI:5297176

hm1

• Allelic Composition: Panx1^tm1.1Fama/Panx1^tm1.1Fama
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

decreased susceptibility to pharmacologically induced seizures ( J:177671 )

• induced by kainic acid

abnormal neuron physiology ( J:177671 )

• potassium-stimulated hippocampal neurons and astrocytes do not exhibit membrane permeabilization (measured by Yo-Pro dye uptake) and exhibit attenuated ATP release compared with wild-type neurons

• in response to kainic acid, mice fail to exhibit membrane permeabilization (measured by Yo-Pro dye uptake) and exhibit decreased ATP release unlike wild-type mice

hearing/vestibular/ear

hearing/vestibular/ear phenotype ( J:178055 )

N • calcium signal propagation in cochlear cultures is normal

N • sensitivity to extracellular calcium and ectonucleaotidase activity is normal

behavior/neurological

decreased susceptibility to pharmacologically induced seizures ( J:177671 )

• induced by kainic acid

Genotype
MGI:5311017

cx2

• Allelic Composition: Panx1^tm1.1Fama/Panx1^tm1.1Fama; Panx2^tm1.1Mony/Panx2^tm1.1Mony
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Panx1^tm1.1Fama/Panx1^tm1.1Fama Panx2^tm1.1Mony/Panx2^tm1.1Mony mice are protected in a stroke model

nervous system

decreased susceptibility to ischemic brain injury ( J:180516 )

• in a stroke model using middle cerebral artery occlusion, mice exhibit decreased neurological deficits (right turns and latency to move) and infarct volume size compared with wild-type mice

• however, mice exhibit normal production of mature IL1B in cerebral ischemia

decreased cerebral infarct size ( J:180516 )

abnormal channel response ( J:180516 )

• neuron dye release to measure channel function is completely blocked unlike in wild-type cells

• however, mice exhibit normal channel function in astrocytes and cortical spreading depolarization

homeostasis/metabolism

decreased susceptibility to ischemic brain injury ( J:180516 )

• in a stroke model using middle cerebral artery occlusion, mice exhibit decreased neurological deficits (right turns and latency to move) and infarct volume size compared with wild-type mice

• however, mice exhibit normal production of mature IL1B in cerebral ischemia

decreased cerebral infarct size ( J:180516 )

Genotype
MGI:5311018

cx3

• Allelic Composition: Panx1^tm1.1Fama/Panx1⁺; Panx2^tm1.1Mony/Panx2⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

nervous system

abnormal channel response ( J:180516 )

• neuron dye release to measure channel function is slightly decreased compared to in wild-type cells