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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Sema5atm1.2Alk
targeted mutation 1.2, Alex L Kolodkin
MGI:5284881
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Sema5atm1.2Alk/Sema5atm1.2Alk involves: 129 * BALB/cJ * C57BL/6 MGI:5284887
cx2
Sema5atm1.2Alk/Sema5atm1.2Alk
Sema5btm1.2Alk/Sema5btm1.2Alk
involves: 129 * BALB/cJ * C57BL/6 MGI:5284889
cx3
Sema5atm1.2Alk/Sema5atm1.2Alk
Sema5btm1.2Alk/Sema5btm1.2Alk
Tg(Thy1-EGFP)MJrs/0
involves: 129 * BALB/cJ * C57BL/6 * CBA MGI:5284892


Genotype
MGI:5284887
hm1
Allelic
Composition
Sema5atm1.2Alk/Sema5atm1.2Alk
Genetic
Background
involves: 129 * BALB/cJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sema5atm1.2Alk mutation (1 available); any Sema5a mutation (43 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
N
• viable, unlike mice homozygous for Sema5atm1Awp




Genotype
MGI:5284889
cx2
Allelic
Composition
Sema5atm1.2Alk/Sema5atm1.2Alk
Sema5btm1.2Alk/Sema5btm1.2Alk
Genetic
Background
involves: 129 * BALB/cJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sema5atm1.2Alk mutation (1 available); any Sema5a mutation (43 available)
Sema5btm1.2Alk mutation (1 available); any Sema5b mutation (63 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
vision/eye
• dopaminergic amacrine cells exhibit dramatic mistargeting within both the inner nuclear layer and the outer plexiform layer
• Slc17a8-expressing amacrine cells show severe neurite mistargeting within both the inner plexiform layer and inner nuclear layer
• selective disruption of OFF but not ON arbors of amacrine cells in the retina
• ectopic band of bipolar cell axon terminals the colocalize with aberrantly projecting axon terminals of cone OFF bipolar cells within the inner nuclear layer
• cone OFF bipolar cells exhibit robust axon termination defects within the inner nuclear layer
• however, rod ON bipolar cells display mostly normal axonal terminations with only a very few mislocalized axon terminals within the inner nuclear layer
• type 3 OFF cone bipolar cell axonal terminations are more disorganized, exhibiting ectopic axonal terminations within the S1 sublamina or the inner nuclear layer
• at P3-P4 amacrine cells and calbindin positive retinal ganglion cell subtypes extend neurites toward the outer neuroblastic layer
• at P7 calbindin positive neurites extend within the inner nuclear layer forming an ectopic plexiform layer
• cholinergic amacrine cells and calretinin positive cells extend neurites within the inner nuclear layer at P3-P4 and P7
• at P7 calbindin positive neurites extend within the inner nuclear layer forming an ectopic plexiform layer
• M1-type intrinsically photosensitive RGC (ipRGC) dendrites fail to stratify in the S1 sublamina of the inner plexiform layer (IPL) and instead arborize in the inner nuclear layer and outer plexiform layer
• dendritic stratification of ipRGC subtypes in distinct IPL sublaminae at P14 is selectively disrupted
• selective disruption of OFF but not ON arbors of RGCs in the retina
• however, no defects in RGC axonal targeting are seen
• the ectopic plexiform layer creates a discontinuity among the Pax6 positive nuclei
• minor displacement of nuclei within the inner plexiform layer
• retinas show about a 5 fold increase in retinal ganglion cells that exhibit spontaneous neural activity but do not respond to whole field stimuli
• retinal ganglion cells display an ON-OFF index distribution that is heavily skewed toward ON responses
• the amplitude of the b wave is significantly smaller
• however, the a wave is similar to double heterozygous controls

nervous system
• severe defects in stereotypic neurite arborization of multiple amacrine cell types
• dopaminergic amacrine cells exhibit dramatic mistargeting within both the inner nuclear layer and the outer plexiform layer
• all amacrine cells, calretinin positive cells and calbindin positive cells exhibit pronounced ectopic neurite extension toward the outer retina
• M1-type intrinsically photosensitive retinal ganglion cell dendrites (ipRGC) fail to stratify in the S1 sublamina of the inner plexiform layer (IPL) and instead arborize in the inner nuclear layer and outer plexiform layer
• dendritic stratification of ipRGC subtypes in distinct IPL sublaminae at P14 is selectively disrupted
• selective disruption of OFF but not ON arbors of amacrine and retinal ganglion cells in the retina
• dopaminergic amacrine cells exhibit dramatic mistargeting within both the inner nuclear layer and the outer plexiform layer
• Slc17a8-expressing amacrine cells show severe neurite mistargeting within both the inner plexiform layer and inner nuclear layer
• selective disruption of OFF but not ON arbors of amacrine cells in the retina
• ectopic band of bipolar cell axon terminals the colocalize with aberrantly projecting axon terminals of cone OFF bipolar cells within the inner nuclear layer
• cone OFF bipolar cells exhibit robust axon termination defects within the inner nuclear layer
• however, rod ON bipolar cells display mostly normal axonal terminations with only a very few mislocalized axon terminals within the inner nuclear layer
• type 3 OFF cone bipolar cell axonal terminations are more disorganized, exhibiting ectopic axonal terminations within the S1 sublamina or the inner nuclear layer
• M1-type intrinsically photosensitive RGC (ipRGC) dendrites fail to stratify in the S1 sublamina of the inner plexiform layer (IPL) and instead arborize in the inner nuclear layer and outer plexiform layer
• dendritic stratification of ipRGC subtypes in distinct IPL sublaminae at P14 is selectively disrupted
• selective disruption of OFF but not ON arbors of RGCs in the retina
• however, no defects in RGC axonal targeting are seen




Genotype
MGI:5284892
cx3
Allelic
Composition
Sema5atm1.2Alk/Sema5atm1.2Alk
Sema5btm1.2Alk/Sema5btm1.2Alk
Tg(Thy1-EGFP)MJrs/0
Genetic
Background
involves: 129 * BALB/cJ * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sema5atm1.2Alk mutation (1 available); any Sema5a mutation (43 available)
Sema5btm1.2Alk mutation (1 available); any Sema5b mutation (63 available)
Tg(Thy1-EGFP)MJrs mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
vision/eye
• about 85% of labeled retinal ganglion cells have dendrites that arborize broadly within the inner plexiform layer, extending into the inner nuclear layer, outer plexiform layer, and, in a few cases, the outer nuclear layer

nervous system
• about 85% of labeled retinal ganglion cells have dendrites that arborize broadly within the inner plexiform layer, extending into the inner nuclear layer, outer plexiform layer, and, in a few cases, the outer nuclear layer
• about 85% of labeled retinal ganglion cells have dendrites that arborize broadly within the inner plexiform layer, extending into the inner nuclear layer, outer plexiform layer, and, in a few cases, the outer nuclear layer





Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
01/12/2022
MGI 6.17
The Jackson Laboratory