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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Zdhhc9tm1Lex
targeted mutation 1, Lexicon Pharmaceuticals
MGI:5007410
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Zdhhc9tm1Lex/Y
Nrastm1Tyj/Nrastm1Tyj
Tg(Mx1-cre)1Cgn/0
involves: 129S5/SvEvBrd * C57BL/6 * CBA MGI:6771589
ot2
Zdhhc9tm1Lex/Y B6.129S5-Zdhhc9tm1Lex MGI:6315636
ot3
Zdhhc9tm1Lex/Y involves: 129S5/SvEvBrd * C57BL/6 MGI:6771586


Genotype
MGI:6771589
cn1
Allelic
Composition
Zdhhc9tm1Lex/Y
Nrastm1Tyj/Nrastm1Tyj
Tg(Mx1-cre)1Cgn/0
Genetic
Background
involves: 129S5/SvEvBrd * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nrastm1Tyj mutation (1 available); any Nras mutation (42 available)
Tg(Mx1-cre)1Cgn mutation (7 available)
Zdhhc9tm1Lex mutation (2 available); any Zdhhc9 mutation (6 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
neoplasm
• when bone marrow (BM) cells from polyinosinic-polycytidylic acid (pIpC)-treated mutant mice (CD45.2) are transplanted into lethally irradiated (CD45.1) recipient mice, less than 70% of recipients of mutant donor BM cells develop T cell acute lymphoblastic leukemia (T-ALL) with a slower progression and a median survival of 390 days post-transplantation versus 202 days for recipients of wild-type donor BM cells
• after oncogenic Nras activation by pIpC injection, mice develop a chronic myelomonocytic leukemia (CMML)-like disease with a significantly slower progression and a median survival of 173 days post-injection versus 152 days for control mice




Genotype
MGI:6315636
ot2
Allelic
Composition
Zdhhc9tm1Lex/Y
Genetic
Background
B6.129S5-Zdhhc9tm1Lex
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Zdhhc9tm1Lex mutation (2 available); any Zdhhc9 mutation (6 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
behavior/neurological
• mice show impaired task acquisition in the Morris water maze indicating spatial learning deficits
• however, mice exhibit normal behavior in the probe trial indicating normal reference memory
• in the open field test, mice spend more time in the inner zone and subsequently less time near the walls during the habituation period but not during the test period indicating reduced anxiety levels
• in the elevated plus maze, mice spend more time exploring the more aversive open arms and the final third of the arms and less time exploring the closed arms
• mice exhibit reduced acoustic startle reactivity, beginning to show a startle response at decibel levels higher than 85 dB
• however, mice display a similar pattern of prepulse inhibition as wild-type mice, indicating normal sensorimotor gating and hearing ability

growth/size/body
• mice are lighter than wild-type mice

muscle
• in the hanging wire test, mice spend a shorter period of time hanging on the wire, indicative of hypotonia
• however, mice perform similar to wild-type mice on the rotarod test

nervous system
• mice have on average 30% reduction in the total volume of the corpus callosum
• however, no change in hippocampus size is seen

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
syndromic X-linked intellectual disability Raymond type DOID:0060824 OMIM:300799
J:268248




Genotype
MGI:6771586
ot3
Allelic
Composition
Zdhhc9tm1Lex/Y
Genetic
Background
involves: 129S5/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Zdhhc9tm1Lex mutation (2 available); any Zdhhc9 mutation (6 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
hematopoietic system
N
• under homeostatic conditions, hematopoiesis is normal with no significant changes in liver, spleen and thymus weight, absolute numbers of leukocytes, erythrocytes, platelets and hemoglobin level in peripheral blood, or bone marrow (BM) and spleen cellularity
• normal frequencies of LSKs (Lineage-/Sca1+/c-Kit+), SLAMs (CD150+/CD48-/Lineage-/Sca1+/c-Kit+), B cells (CD19+and B220+), myeloid cells (Mac-1+ and Gr-1+) and T cells (CD3+) in BM
• normal clonogenic potential of hematopoietic stem cells (HSCs) and progenitors
• after a single dose of 5-fluorouracil (150 mg/kg) treatment, mice show a slightly faster recovery of peripheral leukocytes than wild-type controls
• after two doses 5-fluorouracil, reduction in peripheral leukocyte count is moderately but significantly slower than in wild-type controls
• Ras palmitoylation is significantly reduced but not completely abolished in bone marrow cells from mutant mice, as shown by an acyl-biotinyl exchange assay

homeostasis/metabolism
• after two doses 5-fluorouracil treatment, death is moderately but significantly slower than in wild-type controls
• after two doses 5-fluorouracil treatment, reduction in peripheral leukocyte count is moderately but significantly slower than in wild-type controls

immune system
• after a single dose of 5-fluorouracil (150 mg/kg) treatment, mice show a slightly faster recovery of peripheral leukocytes than wild-type controls
• after two doses 5-fluorouracil, reduction in peripheral leukocyte count is moderately but significantly slower than in wild-type controls

mortality/aging
• after two doses 5-fluorouracil treatment, death is moderately but significantly slower than in wild-type controls





Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
06/23/2022
MGI 6.20
The Jackson Laboratory