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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Tg(Adipoq-cre/ERT2)1Soff
transgene insertion 1, Stefan Offermanns
MGI:4946615
Summary 1 genotype
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Med19tm1c(EUCOMM)Wtsi/Med19tm1c(EUCOMM)Wtsi
Tg(Adipoq-cre/ERT2)1Soff/0
involves: C57BL/6J * C57BL/6N MGI:6505536


Genotype
MGI:6505536
cn1
Allelic
Composition
Med19tm1c(EUCOMM)Wtsi/Med19tm1c(EUCOMM)Wtsi
Tg(Adipoq-cre/ERT2)1Soff/0
Genetic
Background
involves: C57BL/6J * C57BL/6N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Med19tm1c(EUCOMM)Wtsi mutation (0 available); any Med19 mutation (15 available)
Tg(Adipoq-cre/ERT2)1Soff mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
growth/size/body
N
• mice show no significant change in body weight at 21 days after a 5-day tamoxifen treatment initiated at 12 weeks of age

adipose tissue
• brown adipose tissue (BAT) accumulates unilocular lipid droplets, indicating whitening of brown fat
• Oil red O staining confirmed that pockets of white-adipocyte-like cells within BAT are adipocytes
• marked reduction of white adipocytes at 21 days after tamoxifen treatment
• irregularly sized white adipocytes in residual white fat tissue at 21 days after tamoxifen treatment
• dramatic reduction in gonadal WAT tissue (gWAT) mass at 21 days after tamoxifen treatment
• dramatic reduction in inguinal WAT tissue (iWAT) mass at 21 days after tamoxifen treatment
• dramatic reduction in white adipose tissue mass at 21 days after tamoxifen treatment
• loss of WAT due to increased apoptosis and macrophage infiltration
• accumulation of apoptotic cells in iWAT after tamoxifen treatment, as determined by cleaved caspace-3 staining
• increased white adipose tissue (WAT) inflammation with significant macrophage infiltration in gWAT and iWAT after tamoxifen treatment

homeostasis/metabolism
• surprisingly, lipolytic activity is impaired in inguinal WAT tissue (iWAT) explants, as shown in a lipolysis assay
• mRNA expression of lipolysis genes is reduced in iWAT

immune system
• increased white adipose tissue (WAT) inflammation with significant macrophage infiltration in gWAT and iWAT after tamoxifen treatment

cellular
• accumulation of apoptotic cells in iWAT after tamoxifen treatment, as determined by cleaved caspace-3 staining





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last database update
01/12/2022
MGI 6.17
The Jackson Laboratory