Mouse Genome Informatics
hm1
    Pknox1tm1Fbla/Pknox1tm1Fbla
B6.Cg-Pknox1tm1Fbla
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging

embryogenesis
• as determined by marker expression
• at E7.5 to E7.75, no embryonic derivatives were detected by marker expression unlike in wild-type mice
• as determined by marker expression
• the number of Oct4+ cells is reduced at E6.75 through E8.5 by apoptosis compared to in wild-type mice
• at E7.5 to E7.75, marker expression determined that extra-embryonic compartments are restricted to residual cells of extra-embryonic endoderm and derivatives of visceral ectoderm unlike in wild-type mice
• absent as determined by marker expression

growth/size


Mouse Genome Informatics
cx2
    Cdkn2atm1.1Brn/Cdkn2atm1.1Brn
Pknox1tm1Fbla/Pknox1tm1Fbla
Trp53tm1Tyj/Trp53tm1Tyj

involves: 129/Sv * 129P2/OlaHsd * 129S2/SvPas * C57BL/6
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• no mice are recovered (no time of death given)


Mouse Genome Informatics
cx3
    Cdkn2atm1.1Brn/Cdkn2atm1.1Brn
Pknox1tm1Fbla/Pknox1tm1Fbla

involves: 129/Sv * 129P2/OlaHsd * C57BL/6
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
embryogenesis
• mice exhibit the same reduced Oct4 expression domain as in Pknox1tm1Fbla homozygotes


Mouse Genome Informatics
cx4
    Pknox1tm1Fbla/Pknox1tm1Fbla
Trp53tm1Tyj/Trp53tm1Tyj

involves: 129S2/SvPas * C57BL/6
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• mice are almost reabsorbed between E9.5 and E10.5

embryogenesis
• anterior visceral endoderm and primitive streak formation are partially rescued compared to in Pknox1tm1Fbla homozygotes
• the Oct4 expression domain is expanded with a decrease in apoptosis compared to in Pknox1tm1Fbla homozygotes
• the extra-embryonic ectoderm is better organized than in Pknox1tm1Fbla homozygotes

growth/size


Mouse Genome Informatics
cx5
    Atmtm1Fwa/Atmtm1Fwa
Pknox1tm1Fbla/Pknox1+

involves: 129S4/SvJae * C57BL/6
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
embryogenesis
• in 2 of 4 mice

growth/size
• in 2 of 4 mice


Mouse Genome Informatics
cx6
    Atmtm1Fwa/Atmtm1Fwa
Pknox1tm1Fbla/Pknox1tm1Fbla

involves: 129S4/SvJae * C57BL/6
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging

embryogenesis
• at E7.5, Oct4+ cells are almost undetectable in the single mouse generated
• in the single mouse generated

growth/size
• in the single mouse generated