Phenotypes associated with this allele

• Allele Symbol: Wdfy3^{tm1a(KOMP)Mbp}
• Allele Name: targeted mutation 1a, Mouse Biology Program, UC Davis
• Allele ID: MGI:4455365
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Wdfy3^tm1a(KOMP)Mbp/Wdfy3^tm1a(KOMP)Mbp	C57BL/6N-Wdfy3^tm1a(KOMP)Mbp	MGI:5825304
ht2	Wdfy3^tm1a(KOMP)Mbp/Wdfy3^+	C57BL/6N-Wdfy3^tm1a(KOMP)Mbp	MGI:5825305

Genotype
MGI:5825304

hm1

• Allelic Composition: Wdfy3^{tm1a(KOMP)Mbp}/Wdfy3^{tm1a(KOMP)Mbp}
• Genetic Background: C57BL/6N-Wdfy3^{tm1a(KOMP)Mbp}
• Cell Lines: DEPD00523_5_G11

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Wdfy3^{tm1a(KOMP)Mbp}/Wdfy3^{tm1a(KOMP)Mbp} mice exhibit severe neurodevelopmental defects

mortality/aging

perinatal lethality, complete penetrance ( J:225200 )

• homozygotes die perinatally for unknown reasons

nervous system

abnormal neuronal migration ( J:225200 )

• analysis of cortical layer-specific markers Tbr1 (layer VI) and Ctip2 (layer V) revealed focal anomalies in the somatosensory and motor cortices at P0

abnormal forebrain development ( J:225200 )

• newborn pups exhibit more severe neurodevelopmental defects than Wdfy3^disc homozygotes

abnormal cerebral hemisphere morphology ( J:225200 )

• ~13% increase in overall cerebral size relative to wild-type controls

abnormal cerebral cortex morphology ( J:225200 )

• at E18.5, mice exhibit widespread focal cortical dysplasias that disrupt most cortical layers and invade the marginal zone

• focal cortical dysplasias occur at a higher frequency than in Wdfy3^disc homozygotes

abnormal neocortex morphology ( J:225200 )

• newborn brains show an even more drastic thinning and lengthening of the neocortex relative to that in Wdfy3^disc homozygotes

abnormal stratification in cerebral cortex ( J:225200 )

• abnormal cortical layer formation through intermingling of Ctip2+ neurons with Tbr1+ neurons in layer VI

thin cerebral cortex ( J:225200 )

• 16% reduction in neocortical thickness only in the most lateral position, with a trend towards thinning throughout the cortex

ectopic cortical neuron ( J:225200 )

• heterotopias are found in the somatosensory cortex and more dorsally in the motor area and can affect the hippocampus, unlike in Wdfy3^disc homozygotes where they are restricted to lateral aspects of the somatosensory area of the neocortex

cellular

abnormal neuronal migration ( J:225200 )

• analysis of cortical layer-specific markers Tbr1 (layer VI) and Ctip2 (layer V) revealed focal anomalies in the somatosensory and motor cortices at P0

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
autism spectrum disorder		DOID:0060041		J:225200

Genotype
MGI:5825305

ht2

• Allelic Composition: Wdfy3^{tm1a(KOMP)Mbp}/Wdfy3⁺
• Genetic Background: C57BL/6N-Wdfy3^{tm1a(KOMP)Mbp}
• Cell Lines: DEPD00523_5_G11

nervous system

abnormal neocortex morphology ( J:225200 )

• newborn heterozygotes show a mild but significant (~19%) increase in neocortical length relative to wild-type controls, unlike Wdfy3^disc heterozygotes

• however, overall neocortical size is not significantly increased and no focal cortical dysplasias are observed