Phenotypes associated with this allele

• Allele Symbol: Trdn^tm1.2Isma
• Allele Name: targeted mutation 1.2, Isabelle Marty
• Allele ID: MGI:4437311
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Trdn^tm1.2Isma/Trdn^tm1.2Isma	involves: 129S2/SvPas * C57BL/6 * SJL	MGI:4437314

Genotype
MGI:4437314

hm1

• Allelic Composition: Trdn^tm1.2Isma/Trdn^tm1.2Isma
• Genetic Background: involves: 129S2/SvPas * C57BL/6 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Abnormal skeletal muscle fiber triad morphology in Trdn^tm1.2Isma/Trdn^tm1.2Isma mice

muscle

abnormal sarcoplasmic reticulum morphology ( J:157794 )

• terminal cisternae volume is decreased compared to in wild-type sarcoplasmic reticulum

abnormal skeletal muscle fiber triad morphology ( J:157794 )

• some triads in the extensor digitorum longus exhibit longitudinal or oblique orientation compared with transversal orientation in wild-type mice

skeletal muscle hypertrophy ( J:157794 )

• gastrocnemius muscle volume is increased compared to in wild-type mice without a change in body weight

abnormal muscle physiology ( J:157794 )

• calcium reuptake in muscle cells is slower than in wild-type cells

• in the presence of extracellular calcium, maximal amplitude of calcium release is lower than in similarly treated wild-type cells

• however, muscle energy metabolism is normal

abnormal muscle electrophysiology ( J:157794 )

• single twitches and tetani of extensor digitorum longus and soleus muscles are smaller in amplitude than wild-type muscle

• following a series of tetani to induce fatigue, decline in tetanic force is slower than in wild-type muscle

• following a series of tetani to induce fatigue, peak tetanic force undergoes a sudden drop followed by a plateau-like phase early in the run unlike similarly treated wild-type muscle

• through a 6-min stimulation protocol, muscles exhibit a lower isometric force production and larger increase in force to reach maximal levels compared with similarly treated wild-type muscle

• after a 6-min stimulation protocol, muscles exhibit a smaller decrease compared with similarly treated wild-type muscle

behavior/neurological

decreased grip strength ( J:157794 )

• mice exhibit impaired performance in a free walk on a wire stretched between two rods or a hung gripping a cross-wired surface compared with wild-type mice

growth/size/body

growth/size/body region phenotype ( J:157794 )

N • mice exhibit normal body weight