Phenotypes associated with this allele

• Allele Symbol: Rnaseh2b^{tm1a(EUCOMM)Wtsi}
• Allele Name: targeted mutation 1a, Wellcome Trust Sanger Institute
• Allele ID: MGI:4432164
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rnaseh2b^tm1a(EUCOMM)Wtsi/Rnaseh2b^tm1a(EUCOMM)Wtsi	involves: C57BL/6N	MGI:5431541
cx2	Rnaseh2b^tm1a(EUCOMM)Wtsi/Rnaseh2b^tm1a(EUCOMM)Wtsi Trp53^tm1Mlh/Trp53^tm1Mlh	involves: 129P2/OlaHsd * C57BL/6N	MGI:5431542
cx3	Ifnar1^tm1Agt/Ifnar1^tm1Agt Rnaseh2b^tm1a(EUCOMM)Wtsi/Rnaseh2b^tm1a(EUCOMM)Wtsi	involves: 129S2/SvPas * C57BL/6N	MGI:5911414

Genotype
MGI:5431541

hm1

• Allelic Composition: Rnaseh2b^{tm1a(EUCOMM)Wtsi}/Rnaseh2b^{tm1a(EUCOMM)Wtsi}
• Genetic Background: involves: C57BL/6N
• Cell Lines: EPD0087_4_A02

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cardiovascular system

abnormal blood vessel morphology ( J:186986 )

• extended vessels

hemorrhage ( J:186986 )

mortality/aging

preweaning lethality ( J:186180 )

• crosses between heterozygotes fail to produce offspring

perinatal lethality, complete penetrance ( J:186986 )

• despite being present in Mendelian ratios at E14.5, mice die prior to or around birth

endocrine/exocrine glands

thymus hypoplasia ( J:186986 )

• hypocellular E18.5

embryo

embryonic growth retardation ( J:186180 )

decreased embryo size ( J:186180 )

cellular

decreased cell proliferation ( J:186180 )

• of mouse embryonic fibroblasts

decreased fibroblast proliferation ( J:186986 )

• of E14.5 fibroblasts, becoming more pronounced with increasing passage number

• however, no increase in apoptosis is observed

hematopoietic system

thymus hypoplasia ( J:186986 )

• hypocellular E18.5

homeostasis/metabolism

edema ( J:186986 )

growth/size/body

embryonic growth retardation ( J:186180 )

decreased embryo size ( J:186180 )

decreased birth body size ( J:186986 )

• as early as E14.5

immune system

thymus hypoplasia ( J:186986 )

• hypocellular E18.5

liver/biliary system

liver hypoplasia ( J:186986 )

• hypocellular E18.5

Genotype
MGI:5431542

cx2

• Allelic Composition: Rnaseh2b^{tm1a(EUCOMM)Wtsi}/Rnaseh2b^{tm1a(EUCOMM)Wtsi}; Trp53^tm1Mlh/Trp53^tm1Mlh
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6N
• Cell Lines: EPD0087_4_A02

cellular

cellular phenotype ( J:186180 )

N • reduced mouse embryonic fibroblasts cell proliferation is rescued compared to in Rnaseh2b^{tm1a(EUCOMM)Wtsi} homozygotes

abnormal cell nucleus morphology ( J:186180 )

• micronuclei in mouse embryonic fibroblasts

increased cellular sensitivity to alkylating agents ( J:186180 )

chromosomal instability ( J:186180 )

• chromosomal rearrangements in mouse embryonic fibroblasts

embryo

embryo phenotype ( J:186180 )

N • defects in embryo axis, somite development and allantois are rescued compared to in Rnaseh2b^{tm1a(EUCOMM)Wtsi} homozygotes

embryonic growth retardation ( J:186180 )

• partially rescued

decreased embryo size ( J:186180 )

• partially rescued at E9.5

abnormal neural tube closure ( J:186180 )

• in some mice

growth/size/body

embryonic growth retardation ( J:186180 )

• partially rescued

decreased embryo size ( J:186180 )

• partially rescued at E9.5

nervous system

abnormal neural tube closure ( J:186180 )

• in some mice

Genotype
MGI:5911414

cx3

• Allelic Composition: Ifnar1^tm1Agt/Ifnar1^tm1Agt; Rnaseh2b^{tm1a(EUCOMM)Wtsi}/Rnaseh2b^{tm1a(EUCOMM)Wtsi}
• Genetic Background: involves: 129S2/SvPas * C57BL/6N
• Cell Lines: EPD0087_4_A02

mortality/aging

perinatal lethality, complete penetrance ( J:186986 )

growth/size/body

decreased fetal size ( J:186986 )

• at E17.5