Analysis Tools
nervous system
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• no acceleration of degeneration is observed compared to Tg(Camk2a-tTA)1Mmay/Tg(tetO-LRRK2*G2019S)E3Cai mice (G2019S)
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Allele Symbol Allele Name Allele ID |
Tg(tetO-LRRK2*G2019S)E3Cai transgene insertion E3, Huaibin Cai MGI:4420755 |
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| Summary |
4 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• no acceleration of degeneration is observed compared to Tg(Camk2a-tTA)1Mmay/Tg(tetO-LRRK2*G2019S)E3Cai mice (G2019S)
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• starting at 1 year, mice appear to gain less body weight than non-transgenic and Tg(tetO-LRRK2*G2019S)E3Cai single mutant mice, but are not different from Tg(Camk2a-tTA)1Mmay animals
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| N |
• mice show normal performance in the rotarod test
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• mice show significantly increased ambulatory activities starting at 12 months of age; at 12 months mice show a trend to increased rearing activities but this does not reach statistical significance
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| N |
• no significant changes in neuron counts are observed compared to non-transgenic controls at the frontal cortex or dorsal striatum in 6- or 20-month old mice
• no increase in reactive astrocytosis or microglial activation are detected in the striatum or cortex at 20 months
• no alpha-synuclein accumulation is observed in neurons at 20 months
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• the Golgi complex is drastically altered in neurons at 1 month, appearing thinner and fragmented
• at 6 months fragmentation of the cis-Golgi apparatus is similar to that in LRRK2WT and A53T/LRRK2 neurons
• at 1 month, the medial/trans-Golgi in neurons is significantly altered
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• brain homogenates moderately elevated levels of ubiquitinated proteins at 18 months compared to non-transgenic controls
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• substantial accumulation of alpha-synuclein is detected in cell bodies at 1 month
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• exacerbated in the striatum compared to A53T mice at 1 month of age
• levels of microglial activation are higher than in A53T/LRRK2WT mice
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• exacerbated in the striatum compared to A53T mice at 1 month of age
• levels of GFAP-positive cells are higher than in A53T/LRRK2WT mice
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• the Golgi complex is drastically altered in neurons at 1 month with severely fragmented
• at 1 month, the medial/trans-Golgi in neurons is severely fragmented
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• in striatal neurons at 1 month, numerous mitochondria have denser matrices and widened cristae compared to controls or A53T mice (seen with 3.5x greater frequency than in A53T mice); these mitochondria are not functional
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• a significant increase in detergent-insoluble high molecular weight (HMW) alpha synuclein is detected in 1 month old mice compared to A53T age-matched mice
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• exacerbated in the striatum compared to A53T mice at 1 month of age
• dramatic loss (>85%) of striatal neurons is seen in the dorsal striatum
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• exacerbated in the striatum compared to A53T mice at 1 month of age
• levels of microglial activation are higher than in A53T/LRRK2WT mice
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• exacerbated in the striatum compared to A53T mice at 1 month of age
• levels of microglial activation are higher than in A53T/LRRK2WT mice
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• in striatal neurons at 1 month, numerous mitochondria have denser matrices and widened cristae compared to controls or A53T mice (seen with 3.5x greater frequency than in A53T mice); these mitochondria are not functional
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• somatic alpha synuclein in neurons is apparent at 1 month
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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