Phenotypes associated with this allele

• Allele Symbol: Gt(ROSA)26Sor^{tm7(CAG-mCherry,-EGFP/tetX)Dym}
• Allele Name: targeted mutation 7, Susan Dymecki
• Allele ID: MGI:4412286
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gt(ROSA)26Sor^tm7(CAG-mCherry,-EGFP/tetX)Dym/Gt(ROSA)26Sor^+ En1^tm2(cre)Wrst/En1^+ Tg(Fev-flpe)1Dym/0	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ	MGI:4412291
cn2	Gt(ROSA)26Sor^tm7(CAG-mCherry,-EGFP/tetX)Dym/Gt(ROSA)26Sor^+ En1^tm2(cre)Wrst/En1^+ Tg(ACTFLPe)9205Dym/0	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6 * SJL	MGI:4412289
cn3	Gt(ROSA)26Sor^tm7(CAG-mCherry,-EGFP/tetX)Dym/Gt(ROSA)26Sor^+ Tg(ACTFLPe)9205Dym/0 Tg(Pcp2-cre)2Mpin/0	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6 * SJL	MGI:4412292
cn4	Gt(ROSA)26Sor^tm7(CAG-mCherry,-EGFP/tetX)Dym/Gt(ROSA)26Sor^+ Tg(ACTFLPe)9205Dym/0 Tg(Atoh1-cre)1Bfri/0	involves: 129S6/SvEvTac * C57BL/6 * CBA * SJL	MGI:4412287
cn5	Gt(ROSA)26Sor^tm7(CAG-mCherry,-EGFP/tetX)Dym/Gt(ROSA)26Sor^+ Tmem163^Tg(ACTB-cre)2Mrt/0 Tg(ACTFLPe)9205Dym/0	involves: 129S6/SvEvTac * C57BL/6 * FVB/N * SJL	MGI:4412288
cn6	Gt(ROSA)26Sor^tm7(CAG-mCherry,-EGFP/tetX)Dym/Gt(ROSA)26Sor^+ Tmem163^Tg(ACTB-cre)2Mrt/0 Tg(Fev-flpe)1Dym/0	involves: 129S6/SvEvTac * FVB/N	MGI:4412290

Genotype
MGI:4412291

cn1

• Allelic Composition: Gt(ROSA)26Sor^{tm7(CAG-mCherry,-EGFP/tetX)Dym}/Gt(ROSA)26Sor⁺; En1^tm2(cre)Wrst/En1⁺; Tg(Fev-flpe)1Dym/0
• Genetic Background: involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

behavior/neurological phenotype ( J:154944 )

N • mice exhibit normal contextual fear conditioning and prepulse mediated inhibition of acoustic startle reflex

decreased anxiety-related response ( J:154944 )

nervous system

abnormal axon morphology ( J:154944 )

• 5HT+ axon varicosities are enlarged compared to in wild-type mice

Genotype
MGI:4412289

cn2

• Allelic Composition: Gt(ROSA)26Sor^{tm7(CAG-mCherry,-EGFP/tetX)Dym}/Gt(ROSA)26Sor⁺; En1^tm2(cre)Wrst/En1⁺; Tg(ACTFLPe)9205Dym/0
• Genetic Background: involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6 * SJL

mortality/aging

perinatal lethality ( J:154944 )

Genotype
MGI:4412292

cn3

• Allelic Composition: Gt(ROSA)26Sor^{tm7(CAG-mCherry,-EGFP/tetX)Dym}/Gt(ROSA)26Sor⁺; Tg(ACTFLPe)9205Dym/0; Tg(Pcp2-cre)2Mpin/0
• Genetic Background: involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6 * SJL

behavior/neurological

tremors ( J:154944 )

• mild tremors during motion

Genotype
MGI:4412287

cn4

• Allelic Composition: Gt(ROSA)26Sor^{tm7(CAG-mCherry,-EGFP/tetX)Dym}/Gt(ROSA)26Sor⁺; Tg(ACTFLPe)9205Dym/0; Tg(Atoh1-cre)1Bfri/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * CBA * SJL

behavior/neurological

impaired balance ( J:154944 )

impaired coordination ( J:154944 )

abnormal gait ( J:154944 )

• aberrant foot print angles, irregular and reduced stride lengths

short stride length ( J:154944 )

nervous system

abnormal synaptic vesicle recycling ( J:154944 )

• parallel fibers accumulate unreleased synaptic vesicles unlike in wild-type cells

abnormal excitatory postsynaptic currents ( J:154944 )

• stimulation of parallel fibers fails to stimulate an excitatory postsynaptic current (EPSC) unlike in similarly treated wild-type mice

• however, extremely high stimulus intensities evoke an EPSC and stimulated parallel fibers propagate action potentials normally

hearing/vestibular/ear

abnormal ear physiology ( J:154944 )

• mice exhibit auditory defects

Genotype
MGI:4412288

cn5

• Allelic Composition: Gt(ROSA)26Sor^{tm7(CAG-mCherry,-EGFP/tetX)Dym}/Gt(ROSA)26Sor⁺; Tmem163^{Tg(ACTB-cre)2Mrt}/0; Tg(ACTFLPe)9205Dym/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * FVB/N * SJL

mortality/aging

perinatal lethality ( J:154944 )

Genotype
MGI:4412290

cn6

• Allelic Composition: Gt(ROSA)26Sor^{tm7(CAG-mCherry,-EGFP/tetX)Dym}/Gt(ROSA)26Sor⁺; Tmem163^{Tg(ACTB-cre)2Mrt}/0; Tg(Fev-flpe)1Dym/0
• Genetic Background: involves: 129S6/SvEvTac * FVB/N

cardiovascular system

decreased heart rate ( J:231745 )

• heart rate is slower by 6-10% at P5 and P8

behavior/neurological

enhanced contextual conditioning behavior ( J:154944 )

increased exploration in new environment ( J:154944 )

decreased anxiety-related response ( J:154944 )

• mice are less averse to open, brightly lit spaces compared with wild-type mice

• female mice exhibit decreased anxiety-related behaviors in open field, zero-maze, and light-dark tests compared with wild-type mice

growth/size/body

decreased body size ( J:231745 )

• pups are smaller than controls by 15-21% at P5 and P8

nervous system

abnormal axon morphology ( J:154944 )

• 5HT+ axon varicosities are enlarged compared to in wild-type mice

increased prepulse inhibition ( J:154944 )

homeostasis/metabolism

abnormal oxygen consumption ( J:231745 )

• oxygen consumption is slightly but significantly lower at P5 and higher at P12

mortality/aging

increased susceptibility to induced morbidity/mortality ( J:231745 )

• autoresuscitation is less effective compared to controls following repeated episodic exposure to anoxia and results in increased mortality at P5 and P8, with only 16% of pups surviving to the endpoint at P12; deaths occur during the anoxic challenge

respiratory system

increased tidal volume ( J:231745 )

• tidal volume is increased by 18-34% at P8 and P12

abnormal breathing pattern ( J:231745 )

• increase in baseline breathing variability at P5

• autoresuscitation is less effective compared to controls following repeated episodic exposure to anoxia and results in increased mortality at P5 and P8, with only 16% of pups surviving to the endpoint at P12

• pups exposed to anoxia that die at P5 take 4 times longer to initiate gasping and 4 times longer to recover heart rate and eupneic breathing to 90% of baseline

hypopnea ( J:231745 )

• pups have slower breathing than controls by 14% at P5 and P8

increased pulmonary ventilation ( J:231745 )

• 28% larger minute ventilation at P12

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
sudden infant death syndrome		DOID:9007	OMIM:272120	J:231745