All Phenotypes Rheb<tm1Pfw> MGI Mouse

About Help FAQ

Phenotypes associated with this allele

Allele Symbol
Allele Name
Allele ID

Rheb^tm1Pfw
targeted mutation 1, Paul Worley
MGI:3850507

Summary

4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Rheb^tm1Pfw/Rheb^tm1Pfw Tg(Amh-cre)8815Reb/0	involves: 129S1/SvImJ * FVB/N	MGI:6460092
cn2	Rheb^tm1Pfw/Rheb^tm1Pfw Tg(Cd4-cre)1Cwi/0	involves: C57BL/6 * DBA/2	MGI:3850517
cn3	Rheb^tm1Pfw/Rheb^tm1Pfw Tg(Nes-cre)1Kln/0	involves: C57BL/6 * SJL	MGI:4939480
cn4	Gt(ROSA)26Sor^{tm1(CAG-Rheb*)Pfw}/Gt(ROSA)26Sor⁺ Rheb^tm1Pfw/Rheb^tm1Pfw Tg(Nes-cre)1Kln/0	involves: C57BL/6 * SJL	MGI:4939481

Allelic Composition	Rheb^tm1Pfw/Rheb^tm1Pfw Tg(Amh-cre)8815Reb/0
Genetic Background	involves: 129S1/SvImJ * FVB/N

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rheb^tm1Pfw mutation (0 available); any Rheb mutation (26 available)
Tg(Amh-cre)8815Reb mutation (2 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

reproductive system phenotype ( J:268375 )

N	• adult males are fertile, have many normal sperm present in the epididymis, and show normal distribution of vimentin filaments in Sertoli cells (SCs) at 2 months of age, suggesting normal SC cytoskeletal organization

oligozoospermia ( J:268375 )

• 70% reduction in sperm count at 2 months of age

abnormal seminiferous tubule morphology ( J:268375 )

• adult mice display only mild morphological alterations in the seminiferous tubules

decreased testis weight ( J:268375 )

• 60% reduction in testis weight at 2 months of age

decreased epididymis weight ( J:268375 )

• 54% reduction in epididymis weight at 2 months of age

cellular

oligozoospermia ( J:268375 )

• 70% reduction in sperm count at 2 months of age

endocrine/exocrine glands

abnormal seminiferous tubule morphology ( J:268375 )

• adult mice display only mild morphological alterations in the seminiferous tubules

decreased testis weight ( J:268375 )

• 60% reduction in testis weight at 2 months of age

Allelic Composition	Rheb^tm1Pfw/Rheb^tm1Pfw Tg(Cd4-cre)1Cwi/0
Genetic Background	involves: C57BL/6 * DBA/2

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rheb^tm1Pfw mutation (0 available); any Rheb mutation (26 available)
Tg(Cd4-cre)1Cwi mutation (10 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

immune system phenotype ( J:150108 )

N	• despite inhibition of TORC1 activity, T cells exhibit normal regulatory T cell differentiation

Allelic Composition	Rheb^tm1Pfw/Rheb^tm1Pfw Tg(Nes-cre)1Kln/0
Genetic Background	involves: C57BL/6 * SJL

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rheb^tm1Pfw mutation (0 available); any Rheb mutation (26 available)
Tg(Nes-cre)1Kln mutation (4 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:168571 )

• most die at 5-6 weeks of age

nervous system

abnormal brain development ( J:168571 )

• increase in the number of oligodendrocyte precursors in the cortex and hippocampus at 3 weeks of age

decreased brain weight ( J:168571 )

• a decrease in brain weight is detected at P5-P9 but not at P1

• by 2-3 weeks of age brain weight is about 50% that of controls

thin cerebral cortex ( J:168571 )

• at 4 weeks of age cortical thickness is about 65% that of controls

• however, no reduction in neuronal counts is detected at P1, P5 or P28

decreased oligodendrocyte number ( J:168571 )

• the number of mature oligodendrocytes is reduced by 60-70% in multiple regions of the brain, including the corpus callosum, cortex, and hippocampus

• the total number of oligodendrocytes (OLIG2+ cells) is reduced especially in the corpus callosum and to a lesser extent in the cortex and hippocampus

abnormal myelin sheath morphology ( J:168571 )

• widespread loss of fine myelin binding protein reactive fibers in all cortical layers and in neuropil regions of the hippocampus

• decrease in the number of meylinated axons in the corpus callosum

demyelination ( J:168571 )

• prominent at 4 weeks of age

• reduced levels of myelin proteins are detected throughout the brain

• decrease in myelination in the dense white matter tracts

growth/size/body

postnatal growth retardation ( J:168571 )

Allelic Composition	Gt(ROSA)26Sor^{tm1(CAG-Rheb*)Pfw}/Gt(ROSA)26Sor⁺ Rheb^tm1Pfw/Rheb^tm1Pfw Tg(Nes-cre)1Kln/0
Genetic Background	involves: C57BL/6 * SJL

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sor^{tm1(CAG-Rheb*)Pfw} mutation (0 available); any Gt(ROSA)26Sor mutation (942 available)
Rheb^tm1Pfw mutation (0 available); any Rheb mutation (26 available)
Tg(Nes-cre)1Kln mutation (4 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

nervous system phenotype ( J:168571 )

N	• brain size, myelination, and oligodendrocyte numbers are similar to controls unlike in mutant mice without the knock-in allele in Gt(ROSA)26Sor

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 04/09/2024 MGI 6.23