Mouse Genome Informatics
cn1
    Tgfbr2tm1.2Hlm/Tgfbr2tm1.2Hlm
Tnfrsf4tm2(cre)Nik/Tnfrsf4+
Tg(TcraBDC2.5,TcrbBDC2.5)1Doi/0

involves: 129 * C57BL/6 * NOD * SJL
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
immune system
N
• absolute number of T reg cells in the spleen and the pancreatic lymph nodes are normal (J:196160)
• development of diabetes is somewhat delayed


Mouse Genome Informatics
cn2
    Card11tm1.1Litt/Card11tm1.1Litt
Tnfrsf4tm2(cre)Nik/Tnfrsf4+

involves: 129P2/OlaHsd * 129X1/SvJ
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
immune system
N
• memory T cell development is unaffected (J:180410)
• impaired "reactivation" of T cells after a second antibody challenge
• decreased cellular infiltration of lungs when rechallenged 6 weeks after initial Ova exposure
• fewer cells in bronchoalveolar lavage fluid
• fewer eosinophiles
• increased number of macrophage
• reduced number of activated T cells after rechallenge (CD4+CD69+)

respiratory system
N
• acute airway response to Ova challenge is unaffected (J:180410)
• decreased cellular infiltration of lungs when rechallenged 6 weeks after initial Ova exposure
• fewer cells in bronchoalveolar lavage fluid
• fewer eosinophiles
• increased number of macrophage
• reduced number of activated T cells after rechallenge (CD4+CD69+)

hematopoietic system
• impaired "reactivation" of T cells after a second antibody challenge


Mouse Genome Informatics
cn3
    Pik3cdtm2.1Tnr/Pik3cdtm2.1Tnr
Tnfrsf4tm2(cre)Nik/Tnfrsf4+

involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
immune system
• following immunization
• following immunization, the ratio of germinal centers per B cell follicle is reduced compared to in similarly treated wild-type mice
• following immunization, the number of NP2-binding antibody forming cells in the bone marrow and spleen is reduced compared to in wild-type mice
• following immunization, high-affinity IgG1 antibody titers are modestly reduced compared to in similarly treated wild-type mice

hematopoietic system
• following immunization
• following immunization, the ratio of germinal centers per B cell follicle is reduced compared to in similarly treated wild-type mice
• following immunization, high-affinity IgG1 antibody titers are modestly reduced compared to in similarly treated wild-type mice


Mouse Genome Informatics
cn4
    Ptentm2.1Ppp/Ptentm2.1Ppp
Tnfrsf4tm2(cre)Nik/Tnfrsf4+

involves: 129S1/Sv * 129X1/SvJ
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
immune system
• following immunization
• 3-fold following immunization
• following immunization, mice exhibit an increased in high-affinity antibody titers compared to in similarly treated wild-type mice

hematopoietic system
• following immunization
• 3-fold following immunization
• following immunization, mice exhibit an increased in high-affinity antibody titers compared to in similarly treated wild-type mice


Mouse Genome Informatics
cn5
    B9d2/Tgfb1tm1Flv/B9d2+
Tnfrsf4tm2(cre)Nik/Tnfrsf4+

involves: 129S6/SvEvTac * 129X1/SvJ
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
immune system
• mild beginning at 5 months with mononuclear cell infiltrates in the mucosal lamina propria of the colon, with slight epithelial hyperplasia, and increased numbers of leukocytes in the mucosa
• intraepithelial lymphocytes from the small intestine exhibit a high frequency of IFN-gamma-producing Th1 cells compared with wild-type mice
• in an experimental autoimmune encephalomyelitis model
• mice exhibit only marginally enhanced activation and differentiation of T cells in the peripheral lymph nodes, spleen, and mesenteric lymph nodes compared with wild-type mice
• mice exhibit small foci of infiltrating mononuclear cells in the liver parenchyma unlike wild-type mice
• mice exhibit small foci of infiltrating mononuclear cells in the lung unlike wild-type mice

growth/size/body
• mild beginning at 5 months

digestive/alimentary system
• slight hyperplasia
• mild beginning at 5 months with mononuclear cell infiltrates in the mucosal lamina propria of the colon, with slight epithelial hyperplasia, and increased numbers of leukocytes in the mucosa

liver/biliary system
• mice exhibit small foci of infiltrating mononuclear cells in the liver parenchyma unlike wild-type mice

respiratory system
• mice exhibit small foci of infiltrating mononuclear cells in the lung unlike wild-type mice

hematopoietic system
• intraepithelial lymphocytes from the small intestine exhibit a high frequency of IFN-gamma-producing Th1 cells compared with wild-type mice
• in an experimental autoimmune encephalomyelitis model
• mice exhibit only marginally enhanced activation and differentiation of T cells in the peripheral lymph nodes, spleen, and mesenteric lymph nodes compared with wild-type mice

cellular
• slight hyperplasia


Mouse Genome Informatics
cn6
    B9d2/Tgfb1tm1Flv/Tgfb1tm2Flv
Tnfrsf4tm2(cre)Nik/Tnfrsf4+

involves: 129S6/SvEvTac * 129X1/SvJ
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
immune system
• mild beginning at 5 months with mononuclear cell infiltrates in the mucosal lamina propria of the colon, with slight epithelial hyperplasia, and increased numbers of leukocytes in the mucosa
• intraepithelial lymphocytes from the small intestine exhibit a high frequency of IFN-gamma-producing Th1 cells compared with wild-type mice
• in an experimental autoimmune encephalomyelitis model
• mice exhibit only marginally enhanced activation and differentiation of T cells in the peripheral lymph nodes, spleen, and mesenteric lymph nodes compared with wild-type mice
• mice exhibit small foci of infiltrating mononuclear cells in the liver parenchyma unlike wild-type mice
• mice exhibit small foci of infiltrating mononuclear cells in the lung unlike wild-type mice

growth/size/body
• mild beginning at 5 months

digestive/alimentary system
• slight hyperplasia
• mild beginning at 5 months with mononuclear cell infiltrates in the mucosal lamina propria of the colon, with slight epithelial hyperplasia, and increased numbers of leukocytes in the mucosa

liver/biliary system
• mice exhibit small foci of infiltrating mononuclear cells in the liver parenchyma unlike wild-type mice

respiratory system
• mice exhibit small foci of infiltrating mononuclear cells in the lung unlike wild-type mice

hematopoietic system
• intraepithelial lymphocytes from the small intestine exhibit a high frequency of IFN-gamma-producing Th1 cells compared with wild-type mice
• in an experimental autoimmune encephalomyelitis model
• mice exhibit only marginally enhanced activation and differentiation of T cells in the peripheral lymph nodes, spleen, and mesenteric lymph nodes compared with wild-type mice

cellular
• slight hyperplasia


Mouse Genome Informatics
cn7
    Fyntm1Sor/Fyntm1Sor
Lcktm1Litt/Lcktm1.1Litt
Tnfrsf4tm2(cre)Nik/Tnfrsf4+

involves: 129S7/SvEvBrd * 129X1/SvJ
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
immune system
• in the thymus and spleen
• CD25+ T cells fail to proliferate when transferred into T cell-deficient recipients unlike similarly treated wild-type cells
• CD25- T cells exhibit reduced proliferation when transferred into T cell deficient recipients compared with similarly treated wild-type cells
• regulatory T cell turnover is impaired and suppressive function is lost

hematopoietic system
• in the thymus and spleen
• CD25+ T cells fail to proliferate when transferred into T cell-deficient recipients unlike similarly treated wild-type cells
• CD25- T cells exhibit reduced proliferation when transferred into T cell deficient recipients compared with similarly treated wild-type cells
• regulatory T cell turnover is impaired and suppressive function is lost


Mouse Genome Informatics
cn8
    Lcktm1Litt/Lcktm1.1Litt
Tnfrsf4tm2(cre)Nik/Tnfrsf4+

involves: 129X1/SvJ
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
immune system
• in the thymus and spleen
• CD25+ T cells fail to proliferate when transferred into T cell-deficient recipients unlike similarly treated wild-type cells
• CD25- T cells exhibit reduced proliferation when transferred into T cell deficient recipients compared with similarly treated wild-type cells
• regulatory T cell turnover is impaired and suppressive function is lost

hematopoietic system
• in the thymus and spleen
• CD25+ T cells fail to proliferate when transferred into T cell-deficient recipients unlike similarly treated wild-type cells
• CD25- T cells exhibit reduced proliferation when transferred into T cell deficient recipients compared with similarly treated wild-type cells
• regulatory T cell turnover is impaired and suppressive function is lost


Mouse Genome Informatics
cn9
    Cd4tm1Nik/Cd4tm1Nik
Tnfrsf4tm2(cre)Nik/Tnfrsf4+

involves: 129X1/SvJ
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
immune system
• while the number of regulatory T cells is normal, they lack CD4 expression
• however, regulatory T cells are capable of suppressing T cell activity in vitro

hematopoietic system
• while the number of regulatory T cells is normal, they lack CD4 expression
• however, regulatory T cells are capable of suppressing T cell activity in vitro


Mouse Genome Informatics
cn10
    Nbr1tm1.1Jmos/Nbr1tm1.1Jmos
Tnfrsf4tm2(cre)Nik/Tnfrsf4+

involves: 129X1/SvJ * C57BL/6J
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
immune system
• in Th1-polarized T cells, IFN-gamma secretion is mildly, if at all, affected compared with similarly treated wild-type cells
• Th2-polarized T cell exhibit impaired IL4 and IL5 secretion compared with similarly treated wild-type cells
• slightly, but not significantly, in CD4+ T cells stimulated with anti-CD3 and anti-CD28 antibodies
• from anti-CD3- and anti-CD28-stimulated CD4+ T cells
• in the bronchoalveolar lavage fluid from ovalbumin challenged mice
• from anti-CD3- and anti-CD28-stimulated CD4+ T cells
• in Th2-polarized T cells
• in the bronchoalveolar lavage fluid from ovalbumin challenged mice
• in Th2-polarized T cells
• in the bronchoalveolar lavage fluid from ovalbumin challenged mice
• following ovalbumin challenge, mice exhibit reduced inflammation (bronchoalveolar lavage total cell number, eosinophil infiltration, and levels of IL4, IL5, and IL13) compared with similarly treated wild-type mice

hematopoietic system
• in Th1-polarized T cells, IFN-gamma secretion is mildly, if at all, affected compared with similarly treated wild-type cells
• Th2-polarized T cell exhibit impaired IL4 and IL5 secretion compared with similarly treated wild-type cells


Mouse Genome Informatics
cx11
    Elavl1tm1.1Atas/Elavl1tm1.1Atas
Tnfrsf4tm2(cre)Nik/Tnfrsf4+

involves: 129X1/SvJ * C57BL/6
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
immune system
• slightly under Th17-polarizing conditions
• adoptive transfer of CD4+ Th17 cells immunized with MOG results in delayed onset and reduced severity of experimental autoimmune encephalomyelitis with reduced in recipient mice compared with when wild-type cells are transferred

hematopoietic system
• slightly under Th17-polarizing conditions