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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Tnfrsf4tm2(cre)Nik
targeted mutation 2, Nigel Killeen
MGI:3843034
Summary 13 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Tgfbr2tm1.2Hlm/Tgfbr2tm1.2Hlm
Tnfrsf4tm2(cre)Nik/Tnfrsf4+
Tg(TcraBDC2.5,TcrbBDC2.5)1Doi/0
involves: 129 * C57BL/6 * NOD * SJL MGI:5514245
cn2
Card11tm1.1Litt/Card11tm1.1Litt
Tnfrsf4tm2(cre)Nik/Tnfrsf4+
involves: 129P2/OlaHsd * 129X1/SvJ MGI:5307043
cn3
Pik3cdtm2.1Tnr/Pik3cdtm2.1Tnr
Tnfrsf4tm2(cre)Nik/Tnfrsf4+
involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6 MGI:4850095
cn4
Ptentm2.1Ppp/Ptentm2.1Ppp
Tnfrsf4tm2(cre)Nik/Tnfrsf4+
involves: 129S1/Sv * 129X1/SvJ MGI:4850096
cn5
B9d2/Tgfb1tm1Flv/Tgfb1tm2Flv
Tnfrsf4tm2(cre)Nik/Tnfrsf4+
involves: 129S6/SvEvTac * 129X1/SvJ MGI:4943571
cn6
B9d2/Tgfb1tm1Flv/B9d2+
Tnfrsf4tm2(cre)Nik/Tnfrsf4+
involves: 129S6/SvEvTac * 129X1/SvJ MGI:4943570
cn7
Fyntm1Sor/Fyntm1Sor
Lcktm1Litt/Lcktm1.1Litt
Tnfrsf4tm2(cre)Nik/Tnfrsf4+
involves: 129S7/SvEvBrd * 129X1/SvJ MGI:4359000
cn8
Pdpk1tm1.1Mlw/Pdpk1tm1.1Mlw
Rag2tm1Fwa/Rag2tm1Fwa
Tnfrsf4tm2(cre)Nik/Tnfrsf4+
Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+
involves: 129S/SvEv * 129X1/SvJ * C57BL/6 MGI:5697630
cn9
Cd4tm1Nik/Cd4tm1Nik
Tnfrsf4tm2(cre)Nik/Tnfrsf4+
involves: 129X1/SvJ MGI:4359002
cn10
Lcktm1Litt/Lcktm1.1Litt
Tnfrsf4tm2(cre)Nik/Tnfrsf4+
involves: 129X1/SvJ MGI:4358999
cn11
Pdpk1tm1.1Mlw/Pdpk1tm1.1Mlw
Tnfrsf4tm2(cre)Nik/Tnfrsf4+
Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+
involves: 129X1/SvJ * C57BL/6 MGI:5697628
cn12
Nbr1tm1.1Jmos/Nbr1tm1.1Jmos
Tnfrsf4tm2(cre)Nik/Tnfrsf4+
involves: 129X1/SvJ * C57BL/6J MGI:4837529
cx13
Elavl1tm1.1Atas/Elavl1tm1.1Atas
Tnfrsf4tm2(cre)Nik/Tnfrsf4+
involves: 129X1/SvJ * C57BL/6 MGI:5566528


Genotype
MGI:5514245
cn1
Allelic
Composition
Tgfbr2tm1.2Hlm/Tgfbr2tm1.2Hlm
Tnfrsf4tm2(cre)Nik/Tnfrsf4+
Tg(TcraBDC2.5,TcrbBDC2.5)1Doi/0
Genetic
Background
involves: 129 * C57BL/6 * NOD * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tgfbr2tm1.2Hlm mutation (0 available); any Tgfbr2 mutation (17 available)
Tg(TcraBDC2.5,TcrbBDC2.5)1Doi mutation (2 available)
Tnfrsf4tm2(cre)Nik mutation (1 available); any Tnfrsf4 mutation (8 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
N
• absolute number of T reg cells in the spleen and the pancreatic lymph nodes are normal
• development of diabetes is somewhat delayed




Genotype
MGI:5307043
cn2
Allelic
Composition
Card11tm1.1Litt/Card11tm1.1Litt
Tnfrsf4tm2(cre)Nik/Tnfrsf4+
Genetic
Background
involves: 129P2/OlaHsd * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Card11tm1.1Litt mutation (0 available); any Card11 mutation (20 available)
Tnfrsf4tm2(cre)Nik mutation (1 available); any Tnfrsf4 mutation (8 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
N
• memory T cell development is unaffected
• impaired "reactivation" of T cells after a second antibody challenge
• decreased cellular infiltration of lungs when rechallenged 6 weeks after initial Ova exposure
• fewer cells in bronchoalveolar lavage fluid
• fewer eosinophiles
• increased number of macrophage
• reduced number of activated T cells after rechallenge (CD4+CD69+)

respiratory system
N
• acute airway response to Ova challenge is unaffected
• fewer cells in bronchoalveolar lavage fluid
• fewer eosinophiles
• increased number of macrophage
• reduced number of activated T cells after rechallenge (CD4+CD69+)
• decreased cellular infiltration of lungs when rechallenged 6 weeks after initial Ova exposure

hematopoietic system
• impaired "reactivation" of T cells after a second antibody challenge




Genotype
MGI:4850095
cn3
Allelic
Composition
Pik3cdtm2.1Tnr/Pik3cdtm2.1Tnr
Tnfrsf4tm2(cre)Nik/Tnfrsf4+
Genetic
Background
involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pik3cdtm2.1Tnr mutation (0 available); any Pik3cd mutation (20 available)
Tnfrsf4tm2(cre)Nik mutation (1 available); any Tnfrsf4 mutation (8 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• following immunization
• following immunization, the ratio of germinal centers per B cell follicle is reduced compared to in similarly treated wild-type mice
• following immunization, the number of NP2-binding antibody forming cells in the bone marrow and spleen is reduced compared to in wild-type mice
• following immunization, high-affinity IgG1 antibody titers are modestly reduced compared to in similarly treated wild-type mice

hematopoietic system
• following immunization
• following immunization, the ratio of germinal centers per B cell follicle is reduced compared to in similarly treated wild-type mice
• following immunization, high-affinity IgG1 antibody titers are modestly reduced compared to in similarly treated wild-type mice




Genotype
MGI:4850096
cn4
Allelic
Composition
Ptentm2.1Ppp/Ptentm2.1Ppp
Tnfrsf4tm2(cre)Nik/Tnfrsf4+
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ptentm2.1Ppp mutation (0 available); any Pten mutation (41 available)
Tnfrsf4tm2(cre)Nik mutation (1 available); any Tnfrsf4 mutation (8 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• following immunization
• 3-fold following immunization
• following immunization, mice exhibit an increased in high-affinity antibody titers compared to in similarly treated wild-type mice

hematopoietic system
• following immunization
• 3-fold following immunization
• following immunization, mice exhibit an increased in high-affinity antibody titers compared to in similarly treated wild-type mice




Genotype
MGI:4943571
cn5
Allelic
Composition
B9d2/Tgfb1tm1Flv/Tgfb1tm2Flv
Tnfrsf4tm2(cre)Nik/Tnfrsf4+
Genetic
Background
involves: 129S6/SvEvTac * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
B9d2/Tgfb1tm1Flv mutation (0 available); any Tgfb1 mutation (16 available)
Tgfb1tm2Flv mutation (0 available); any Tgfb1 mutation (16 available)
Tnfrsf4tm2(cre)Nik mutation (1 available); any Tnfrsf4 mutation (8 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• mild beginning at 5 months with mononuclear cell infiltrates in the mucosal lamina propria of the colon, with slight epithelial hyperplasia, and increased numbers of leukocytes in the mucosa
• mice exhibit only marginally enhanced activation and differentiation of T cells in the peripheral lymph nodes, spleen, and mesenteric lymph nodes compared with wild-type mice
• intraepithelial lymphocytes from the small intestine exhibit a high frequency of IFN-gamma-producing Th1 cells compared with wild-type mice
• in an experimental autoimmune encephalomyelitis model
• mice exhibit small foci of infiltrating mononuclear cells in the liver parenchyma unlike wild-type mice
• mice exhibit small foci of infiltrating mononuclear cells in the lung unlike wild-type mice

growth/size/body
• mild beginning at 5 months

digestive/alimentary system
• slight hyperplasia
• mild beginning at 5 months with mononuclear cell infiltrates in the mucosal lamina propria of the colon, with slight epithelial hyperplasia, and increased numbers of leukocytes in the mucosa

liver/biliary system
• mice exhibit small foci of infiltrating mononuclear cells in the liver parenchyma unlike wild-type mice

respiratory system
• mice exhibit small foci of infiltrating mononuclear cells in the lung unlike wild-type mice

hematopoietic system
• mice exhibit only marginally enhanced activation and differentiation of T cells in the peripheral lymph nodes, spleen, and mesenteric lymph nodes compared with wild-type mice
• intraepithelial lymphocytes from the small intestine exhibit a high frequency of IFN-gamma-producing Th1 cells compared with wild-type mice
• in an experimental autoimmune encephalomyelitis model

cellular
• slight hyperplasia




Genotype
MGI:4943570
cn6
Allelic
Composition
B9d2/Tgfb1tm1Flv/B9d2+
Tnfrsf4tm2(cre)Nik/Tnfrsf4+
Genetic
Background
involves: 129S6/SvEvTac * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
B9d2/Tgfb1tm1Flv mutation (0 available); any B9d2 mutation (3 available)
Tnfrsf4tm2(cre)Nik mutation (1 available); any Tnfrsf4 mutation (8 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• mild beginning at 5 months with mononuclear cell infiltrates in the mucosal lamina propria of the colon, with slight epithelial hyperplasia, and increased numbers of leukocytes in the mucosa
• mice exhibit only marginally enhanced activation and differentiation of T cells in the peripheral lymph nodes, spleen, and mesenteric lymph nodes compared with wild-type mice
• intraepithelial lymphocytes from the small intestine exhibit a high frequency of IFN-gamma-producing Th1 cells compared with wild-type mice
• in an experimental autoimmune encephalomyelitis model
• mice exhibit small foci of infiltrating mononuclear cells in the liver parenchyma unlike wild-type mice
• mice exhibit small foci of infiltrating mononuclear cells in the lung unlike wild-type mice

growth/size/body
• mild beginning at 5 months

digestive/alimentary system
• slight hyperplasia
• mild beginning at 5 months with mononuclear cell infiltrates in the mucosal lamina propria of the colon, with slight epithelial hyperplasia, and increased numbers of leukocytes in the mucosa

liver/biliary system
• mice exhibit small foci of infiltrating mononuclear cells in the liver parenchyma unlike wild-type mice

respiratory system
• mice exhibit small foci of infiltrating mononuclear cells in the lung unlike wild-type mice

hematopoietic system
• mice exhibit only marginally enhanced activation and differentiation of T cells in the peripheral lymph nodes, spleen, and mesenteric lymph nodes compared with wild-type mice
• intraepithelial lymphocytes from the small intestine exhibit a high frequency of IFN-gamma-producing Th1 cells compared with wild-type mice
• in an experimental autoimmune encephalomyelitis model

cellular
• slight hyperplasia




Genotype
MGI:4359000
cn7
Allelic
Composition
Fyntm1Sor/Fyntm1Sor
Lcktm1Litt/Lcktm1.1Litt
Tnfrsf4tm2(cre)Nik/Tnfrsf4+
Genetic
Background
involves: 129S7/SvEvBrd * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Fyntm1Sor mutation (3 available); any Fyn mutation (20 available)
Lcktm1.1Litt mutation (0 available); any Lck mutation (84 available)
Lcktm1Litt mutation (0 available); any Lck mutation (84 available)
Tnfrsf4tm2(cre)Nik mutation (1 available); any Tnfrsf4 mutation (8 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• in the thymus and spleen
• CD25+ T cells fail to proliferate when transferred into T cell-deficient recipients unlike similarly treated wild-type cells
• CD25- T cells exhibit reduced proliferation when transferred into T cell deficient recipients compared with similarly treated wild-type cells
• regulatory T cell turnover is impaired and suppressive function is lost

hematopoietic system
• in the thymus and spleen
• CD25+ T cells fail to proliferate when transferred into T cell-deficient recipients unlike similarly treated wild-type cells
• CD25- T cells exhibit reduced proliferation when transferred into T cell deficient recipients compared with similarly treated wild-type cells
• regulatory T cell turnover is impaired and suppressive function is lost




Genotype
MGI:5697630
cn8
Allelic
Composition
Pdpk1tm1.1Mlw/Pdpk1tm1.1Mlw
Rag2tm1Fwa/Rag2tm1Fwa
Tnfrsf4tm2(cre)Nik/Tnfrsf4+
Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+
Genetic
Background
involves: 129S/SvEv * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1(EYFP)Cos mutation (1 available); any Gt(ROSA)26Sor mutation (336 available)
Pdpk1tm1.1Mlw mutation (0 available); any Pdpk1 mutation (119 available)
Rag2tm1Fwa mutation (41 available); any Rag2 mutation (78 available)
Tnfrsf4tm2(cre)Nik mutation (1 available); any Tnfrsf4 mutation (8 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• mice succumb to disease at 12-16 weeks of age

integument
• marker analysis indicates impaired keratinocyte differentiation
• mice develop mild skin dermatitis

immune system
• mice develop mild skin dermatitis

cellular
• marker analysis indicates impaired keratinocyte differentiation




Genotype
MGI:4359002
cn9
Allelic
Composition
Cd4tm1Nik/Cd4tm1Nik
Tnfrsf4tm2(cre)Nik/Tnfrsf4+
Genetic
Background
involves: 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd4tm1Nik mutation (2 available); any Cd4 mutation (53 available)
Tnfrsf4tm2(cre)Nik mutation (1 available); any Tnfrsf4 mutation (8 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• while the number of regulatory T cells is normal, they lack CD4 expression
• however, regulatory T cells are capable of suppressing T cell activity in vitro

hematopoietic system
• while the number of regulatory T cells is normal, they lack CD4 expression
• however, regulatory T cells are capable of suppressing T cell activity in vitro




Genotype
MGI:4358999
cn10
Allelic
Composition
Lcktm1Litt/Lcktm1.1Litt
Tnfrsf4tm2(cre)Nik/Tnfrsf4+
Genetic
Background
involves: 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lcktm1.1Litt mutation (0 available); any Lck mutation (84 available)
Lcktm1Litt mutation (0 available); any Lck mutation (84 available)
Tnfrsf4tm2(cre)Nik mutation (1 available); any Tnfrsf4 mutation (8 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• in the thymus and spleen
• CD25+ T cells fail to proliferate when transferred into T cell-deficient recipients unlike similarly treated wild-type cells
• CD25- T cells exhibit reduced proliferation when transferred into T cell deficient recipients compared with similarly treated wild-type cells
• regulatory T cell turnover is impaired and suppressive function is lost

hematopoietic system
• in the thymus and spleen
• CD25+ T cells fail to proliferate when transferred into T cell-deficient recipients unlike similarly treated wild-type cells
• CD25- T cells exhibit reduced proliferation when transferred into T cell deficient recipients compared with similarly treated wild-type cells
• regulatory T cell turnover is impaired and suppressive function is lost




Genotype
MGI:5697628
cn11
Allelic
Composition
Pdpk1tm1.1Mlw/Pdpk1tm1.1Mlw
Tnfrsf4tm2(cre)Nik/Tnfrsf4+
Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+
Genetic
Background
involves: 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1(EYFP)Cos mutation (1 available); any Gt(ROSA)26Sor mutation (336 available)
Pdpk1tm1.1Mlw mutation (0 available); any Pdpk1 mutation (119 available)
Tnfrsf4tm2(cre)Nik mutation (1 available); any Tnfrsf4 mutation (8 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• mice develop a wasting syndrome and succumb to disease by 11 weeks of age

growth/size/body

integument
• loss of hypodermal fat
• marker analysis indicates impaired keratinocyte differentiation
• mice with advanced disease show loss of skin barrier integrity at 7-8 weeks of age
• mice develop severe, systemic dermatitis starting at 5 weeks of age
• inflammation is not seen in the lung, liver, kidney or gut
• dermatitis is accompanied by hair loss
• loss of hair follicles
• mild epidermal hyperplasia and microabsceess are seen at 3 weeks of age but not at 10 days of age
• skin contains epidermal scales
• skin of mice with advanced disease contains lesions with epidermal damage, resulting in loss of skin barrier integrity
• dermatitis is accompanied by skin thickening
• increase in dermal fibrosis

immune system
• decrease in the frequency of Foxp3+ CD25+ Tregs with a corresponding increase in Foxp3-CD25+ effector T cells
• mice develop an enlarged spleen
• CD4 T cells exhibit an activated phenotype
• mice develop systemic T helper type 2 immunity
• mice develop peripheral lymphadenopathy
• mice develop severe, systemic dermatitis starting at 5 weeks of age
• inflammation is not seen in the lung, liver, kidney or gut

homeostasis/metabolism
• mice with advanced disease show loss of skin barrier integrity at 7-8 weeks of age

hematopoietic system
• decrease in the frequency of Foxp3+ CD25+ Tregs with a corresponding increase in Foxp3-CD25+ effector T cells
• mice develop an enlarged spleen
• CD4 T cells exhibit an activated phenotype
• mice develop systemic T helper type 2 immunity

cellular
• marker analysis indicates impaired keratinocyte differentiation

adipose tissue
• loss of hypodermal fat




Genotype
MGI:4837529
cn12
Allelic
Composition
Nbr1tm1.1Jmos/Nbr1tm1.1Jmos
Tnfrsf4tm2(cre)Nik/Tnfrsf4+
Genetic
Background
involves: 129X1/SvJ * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nbr1tm1.1Jmos mutation (0 available); any Nbr1 mutation (48 available)
Tnfrsf4tm2(cre)Nik mutation (1 available); any Tnfrsf4 mutation (8 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• in Th1-polarized T cells, IFN-gamma secretion is mildly, if at all, affected compared with similarly treated wild-type cells
• Th2-polarized T cell exhibit impaired IL4 and IL5 secretion compared with similarly treated wild-type cells
• slightly, but not significantly, in CD4+ T cells stimulated with anti-CD3 and anti-CD28 antibodies
• from anti-CD3- and anti-CD28-stimulated CD4+ T cells
• in the bronchoalveolar lavage fluid from ovalbumin challenged mice
• from anti-CD3- and anti-CD28-stimulated CD4+ T cells
• in Th2-polarized T cells
• in the bronchoalveolar lavage fluid from ovalbumin challenged mice
• in Th2-polarized T cells
• in the bronchoalveolar lavage fluid from ovalbumin challenged mice
• following ovalbumin challenge, mice exhibit reduced inflammation (bronchoalveolar lavage total cell number, eosinophil infiltration, and levels of IL4, IL5, and IL13) compared with similarly treated wild-type mice

hematopoietic system
• in Th1-polarized T cells, IFN-gamma secretion is mildly, if at all, affected compared with similarly treated wild-type cells
• Th2-polarized T cell exhibit impaired IL4 and IL5 secretion compared with similarly treated wild-type cells




Genotype
MGI:5566528
cx13
Allelic
Composition
Elavl1tm1.1Atas/Elavl1tm1.1Atas
Tnfrsf4tm2(cre)Nik/Tnfrsf4+
Genetic
Background
involves: 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Elavl1tm1.1Atas mutation (0 available); any Elavl1 mutation (22 available)
Tnfrsf4tm2(cre)Nik mutation (1 available); any Tnfrsf4 mutation (8 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• slightly under Th17-polarizing conditions
• adoptive transfer of CD4+ Th17 cells immunized with MOG results in delayed onset and reduced severity of experimental autoimmune encephalomyelitis with reduced in recipient mice compared with when wild-type cells are transferred

hematopoietic system
• slightly under Th17-polarizing conditions





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last database update
09/20/2016
MGI 6.05
The Jackson Laboratory