Phenotypes associated with this allele

• Allele Symbol: Use1^tm1.2Aha
• Allele Name: targeted mutation 1.2, Akihiro Harada
• Allele ID: MGI:3842571
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Use1^tm1.2Aha/Use1^tm1.2Aha	involves: BALB/cJ * C57BL/6 * SJL	MGI:3842579
cn2	Use1^tm1.1Aha/Use1^tm1.2Aha	involves: BALB/cJ * C57BL/6 * SJL	MGI:3842578
cn3	Use1^tm1.1Aha/Use1^tm1.2Aha Tg(Pcp2-cre)2Mpin/0	involves: 129S1/Sv * 129X1/SvJ * BALB/cJ * C57BL/6 * SJL	MGI:3842577

Genotype
MGI:3842579

hm1

• Allelic Composition: Use1^tm1.2Aha/Use1^tm1.2Aha
• Genetic Background: involves: BALB/cJ * C57BL/6 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality, complete penetrance ( J:147771 )

• die before E8.5

Genotype
MGI:3842578

cn2

• Allelic Composition: Use1^tm1.1Aha/Use1^tm1.2Aha
• Genetic Background: involves: BALB/cJ * C57BL/6 * SJL

cellular

abnormal endoplasmic reticulum morphology ( J:147771 )

• increase in the percentage of MEF cells with vacuolated ER structures and decrease in the percentage of cells with vesiculated ER structures with time following adenoviral cre treatment

increased fibroblast apoptosis ( J:147771 )

• in MEFs after adenoviral cre treatment

increased endoplasmic reticulum stress ( J:147771 )

• expression analysis indicates increased endoplasmic reticulum stress following adenoviral cre treatment

• protein transport from the endoplasmic reticulum to the Golgi complex is impaired

Genotype
MGI:3842577

cn3

• Allelic Composition: Use1^tm1.1Aha/Use1^tm1.2Aha; Tg(Pcp2-cre)2Mpin/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * BALB/cJ * C57BL/6 * SJL

behavior/neurological

ataxia ( J:147771 )

• display cerebellar ataxia after 10 weeks of age

abnormal gait ( J:147771 )

• after 10 weeks of age

nervous system

decreased Purkinje cell number ( J:147771 )