Analysis Tools
normal phenotype
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• mice are viable, fertile, and display no obvious anomalies
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Allele Symbol Allele Name Allele ID |
Cdc25atm1Hpw targeted mutation 1, Helen Piwnica-Worms MGI:3836585 |
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| Summary |
7 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice are viable, fertile, and display no obvious anomalies
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• no difference in villi height is after 4-hydroxytamoxifen treatment observed after in contrast to triple knockout mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
Shortened villi in the small intestine of Cdc25atm1Hpw/Cdc25atm1.1Hpw Cdc25btm1Pjd/Cdc25btm1Pjd Cdc25ctm1Hpw/Cdc25ctm1Hpw Gt(ROSA)26Sortm1(cre/ERT)Brn/Gt(ROSA)26Sor+ (TKO) mice
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• adult mice die within 1 week of initial 4-hydroxytamoxifen (OHT) injection
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• adult mice exhibit significant weight loss (20%) within 1 week of initial tamoxifen injection
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• profound cell atrophy in the crypts is observed in OHT-treated mice; crypts are >40% smaller in mutants than Cdc25a-single knockout controls, with the crypt width being decreased more significantly than the depth
• a 70% loss of epithelial cells per crypt is detected, but no difference in mature Paneth cells is observed
• decreased proliferation of epithelial cells is observed, with no significant increase in apoptotic cell numbers; this results in premature differentiation of cell progenitors below Paneth cell compartments in crypts
• crypts have increased numbers of cells exhibiting early differentiation with no crypt base columnar (CBC) cells
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• villi are lined with shorter, less-dense enterocytes in proximal regions; complete loss of distal villi is observed in some areas
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• significant loss of villus height in proximal and distal regions is observed in mutants after OHT treatment
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• 6 days following the final of 3 consecutive injections of tamoxifen, length of small intestine is shortened by 40% compared to controls
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• profound cell atrophy in the crypts is observed in OHT-treated mice; crypts are >40% smaller in mutants than Cdc25a-single knockout controls, with the crypt width being decreased more significantly than the depth
• a 70% loss of epithelial cells per crypt is detected, but no difference in mature Paneth cells is observed
• decreased proliferation of epithelial cells is observed, with no significant increase in apoptotic cell numbers; this results in premature differentiation of cell progenitors below Paneth cell compartments in crypts
• crypts have increased numbers of cells exhibiting early differentiation with no crypt base columnar (CBC) cells
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mild reduction in length in tamoxifen-treated mice
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• mild reduction in length in tamoxifen-treated mice
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| N |
• tamoxifen-treated mice exhibit normal body weight
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• tamoxifen-treated mice exhibit normal intestine morphology
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| N |
• tamoxifen-treated mice exhibit normal body weight
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• 2 of 5 tamoxifen-treated mice die by day 8
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• in tamoxifen-treated mice
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• tamoxifen-treated mice exhibit premature differentiation of small intestine crypt cells compared with control mice
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• tamoxifen-treated mice exhibit decreased S-phase cells in crypts compared with control mice
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• reduced length in tamoxifen-treated mice
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• reduced length in tamoxifen-treated mice
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• tamoxifen treated mice exhibit a reduction in epithelium cells in the small intestine crypts compared with control mice
• however, the number of Paneth cells is normal
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• in tamoxifen-treated mice
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• in tamoxifen-treated mice
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• tamoxifen treated mice exhibit a reduction in epithelium cells in the small intestine crypts compared with control mice
• however, the number of Paneth cells is normal
|
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• in tamoxifen-treated mice
|
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• tamoxifen-treated mice exhibit premature differentiation of small intestine crypt cells compared with control mice
|
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• tamoxifen-treated mice exhibit decreased S-phase cells in crypts compared with control mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• 5 of 11 tamoxifen-treated mice die by day 8
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• in tamoxifen-treated mice
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• tamoxifen-treated mice exhibit premature differentiation of small intestine crypt cells compared with control mice
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• tamoxifen-treated mice exhibit decreased S-phase cells in crypts compared with control mice
|
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• reduced length in tamoxifen-treated mice
|
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• reduced length in tamoxifen-treated mice
|
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• tamoxifen treated mice exhibit a reduction in epithelium cells in the small intestine crypts compared with control mice
• however, the number of Paneth cells is normal
|
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• in tamoxifen-treated mice
|
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• in tamoxifen-treated mice
|
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• tamoxifen treated mice exhibit a reduction in epithelium cells in the small intestine crypts compared with control mice
• however, the number of Paneth cells is normal
|
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• in tamoxifen-treated mice
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• tamoxifen-treated mice exhibit premature differentiation of small intestine crypt cells compared with control mice
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• tamoxifen-treated mice exhibit decreased S-phase cells in crypts compared with control mice
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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