Phenotypes associated with this allele

• Allele Symbol: Neto1^tm1Mci
• Allele Name: targeted mutation 1, Roderick R McInnes
• Allele ID: MGI:3836584
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Neto1^tm1Mci/Neto1^tm1Mci	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:3836625
cx2	Neto1^tm1Mci/Neto1^tm1Mci Neto2^tm1Mci/Neto2^tm1Mci	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:5141224

Genotype
MGI:3836625

hm1

• Allelic Composition: Neto1^tm1Mci/Neto1^tm1Mci
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

seizures ( J:145953 )

• Background Sensitivity: within the first two generations of backcrossing to C57BL/6J, mice exhibit myoclonic seizures unlike wild-type mice

• however, after the second generation mice do not exhibit abnormal seizure activity

abnormal excitatory postsynaptic currents ( J:145953 , J:174557 )

• the NMDAR:AMPAR excitatory postsynaptic current (EPSC) ratio is less than in wild-type mice (J:145953)

• NMDAR EPSCs are reduced compared to in wild-type mice (J:145953)

• however, AMPAR ESPCs are normal (J:145953)

• severe deficit in kainate receptor (KAR) mediated EPSCs in mossy fiber-CA3 pyramidal cell synapses (J:174557)

• associational/commissural-CA3 pyramidal cell synapses exhibited reduced NMDA/AMPA EPSC ratios (J:174557)

• however, NMDA/AMPA ratios in mossy fiber-CA3 pyramidal cell synapses are similar to wild-type (J:174557)

short excitatory postsynaptic current decay time ( J:174557 )

• KAR mediated EPSCs show faster decay kinetics

reduced long-term potentiation ( J:145953 )

• theta burst long term potentiation (tbLTP) is reduced 50% compared to in wild-type mice

• treatment with Ro25-6981 decreases tbLTP by 60% unlike in similarly treated wild-type mice

• however, tbLTP is rescued by treatment with CX546

behavior/neurological

abnormal learning/memory/conditioning ( J:145953 )

• mice exhibit impaired hippocampal-dependent spatial learning compared to wild-type mice in a Morris water maze after platform relocation

• mice exhibit impaired learning in the delayed matching-to-place task and the displaced object task compared to wild-type mice

• however, mice exhibit normal novel object recognition and restoration of spatial learning and memory following treatment with CX546

seizures ( J:145953 )

• Background Sensitivity: within the first two generations of backcrossing to C57BL/6J, mice exhibit myoclonic seizures unlike wild-type mice

• however, after the second generation mice do not exhibit abnormal seizure activity

Genotype
MGI:5141224

cx2

• Allelic Composition: Neto1^tm1Mci/Neto1^tm1Mci; Neto2^tm1Mci/Neto2^tm1Mci
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

nervous system

abnormal excitatory postsynaptic currents ( J:174557 )

• deficit in kainate receptor (KAR) mediated EPSCs in mossy fiber-CA3 pyramidal cell synapses is similar to that in mice homozygous for Neto1^tm1Mci alone