About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Hdac3tm1.1Eno
targeted mutation 1.1, Eric N Olson
MGI:3831693
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Hdac3tm1.1Eno/Hdac3tm1.1Eno
Pgrtm2(cre)Lyd/Pgr+
involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ * C57BL/6 MGI:6281662
cn2
Hdac3tm1.1Eno/Hdac3tm1.1Eno
Tg(Myh6-cre)2182Mds/?
involves: 129S/SvEv * C57BL/6 MGI:3831703


Genotype
MGI:6281662
cn1
Allelic
Composition
Hdac3tm1.1Eno/Hdac3tm1.1Eno
Pgrtm2(cre)Lyd/Pgr+
Genetic
Background
involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hdac3tm1.1Eno mutation (0 available); any Hdac3 mutation (7 available)
Pgrtm2(cre)Lyd mutation (0 available); any Pgr mutation (10 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
N
• females exhibit normal ovulation, fertilization, and ovarian histology relative to control mice
• at day 5.5 of gestation (GD 5.5), embryos are free-floating within the uterine lumen and no decidual cells are observed, unlike in control uteri
• following artificially induced decidualization, the ratio of uterine weight to body weight is significantly lower than that in control mice, indicating a decidual defect; mRNA expression of decidualization marker genes Wnt4 and Bmp2 is significantly reduced in the uterus
• at GD 3.5, the expression of stromal markers vimentin and COUP-TFII is significantly reduced in endometrial stromal cells
• immunohistochemical staining showed significantly increased collagen type I protein levels in endometrial stromal cells at GD 3.5, suggesting that endometrial stroma is largely replaced by fibrous tissue
• at GD 5.5, embryos are free-floating within the uterine lumen, unlike in control uteri
• no implantation sites are detected in the uterine horns at GD 5.5
• at GD 3.5, Ki-67 staining revealed significantly altered proliferative responses in the uterine stromal and epithelial compartments: the % of Ki-67+ endometrial epithelial cells is increased whereas the % of Ki-67+ stromal cells is decreased, unlike in control uteri
• expression of estrogen receptor 1 alpha (ESR1) and progesterone receptor (PGR) is significantly reduced in endometrial stromal cells at GD 3.5, indicating decreased progesterone signaling
• adult females are completely sterile primarily due to a uterine defect

embryo
• at day 5.5 of gestation (GD 5.5), embryos are free-floating within the uterine lumen and no decidual cells are observed, unlike in control uteri
• following artificially induced decidualization, the ratio of uterine weight to body weight is significantly lower than that in control mice, indicating a decidual defect; mRNA expression of decidualization marker genes Wnt4 and Bmp2 is significantly reduced in the uterus

homeostasis/metabolism
N
• at GD 3.5, females exhibit normal serum progesterone and estrogen levels relative to control mice
• H&E and Massons trichrome staining revealed increased collagen levels in uterine stromal cells starting at 6 weeks of age
• immunohistochemical staining showed significantly increased collagen type I protein levels in endometrial stromal cells at GD 3.5

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
endometriosis DOID:289 J:269518




Genotype
MGI:3831703
cn2
Allelic
Composition
Hdac3tm1.1Eno/Hdac3tm1.1Eno
Tg(Myh6-cre)2182Mds/?
Genetic
Background
involves: 129S/SvEv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hdac3tm1.1Eno mutation (0 available); any Hdac3 mutation (7 available)
Tg(Myh6-cre)2182Mds mutation (3 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
growth/size/body
• 72% increase in heart weight/body weight ratio by 12 weeks of age
• signs of cardiac hypertrophy by 4 weeks of age
• hypertrophy exacerbated by 12 weeks of age
• both right and left atria are enlarged
• cardiomyocyte hypertrophy, particularly in the left ventricle free wall and the septum

mortality/aging
• significant lethality between 12 and 14 weeks of age
• 100% lethality by 16 weeks

cardiovascular system
N
• normal sinus rhythm
• 72% increase in heart weight/body weight ratio by 12 weeks of age
• signs of cardiac hypertrophy by 4 weeks of age
• hypertrophy exacerbated by 12 weeks of age
• both right and left atria are enlarged
• cardiomyocyte hypertrophy, particularly in the left ventricle free wall and the septum
• increased in both systole and diastole
• reduced fractional shortening of the left ventricle

cellular
• lack normal association with sarcomeres in cardiac muscle
• reduced density of cristae
• 25% reduction in complex I activity
• 39% reduction in NADH oxidase activity
• two fold increase in free radical production

homeostasis/metabolism
• accumulation of neutral lipids in ventricles but not atria
• fasting myocardial triglycerides are increased after 24 hours
• slightly reduced platelet counts

muscle
• reduced fractional shortening of the left ventricle





Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
11/10/2020
MGI 6.16
The Jackson Laboratory