Analysis Tools
immune system
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• from bone marrow-derived macrophage stimulated with LPS
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Allele Symbol Allele Name Allele ID |
Nfkb1tm1Sley targeted mutation 1, Steven C Ley MGI:3822895 |
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| Summary |
5 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• from bone marrow-derived macrophage stimulated with LPS
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• nave CD4+ T cells stimulated in vitro through the TCR only have a third the proliferative response as control mice
• 66% fewer naive CD4+ T cells go into S-phase with 24 hours of TCR stimulation compared to controls
• the S-phase entry defect can be partly overcome by CD28 stimulation
• allogeneic proliferative response of nave CD4+ T cells is reduced 6-fold compared to controls
• the amount of IL-2 required for a half maximal proliferation response is 100-fold higher than in controls
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• CD4+ memory T cells are reduced by 3-fold in the spleen
• splenic CD8+ memory T cells are also reduced
• reconstitution of wild-type mice with both wild-type and mutant bone marrow demonstrate an intrinsic defect of mutant hematopoietic cells to produce CD4+ memory T cells
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• there is a 75% decrease in the number of regulatory T cells found in the spleen, lymph node and thymus of mice
• reconstitution of wild-type mice with both wild-type and mutant bone marrow demonstrate an intrinsic defect of mutant hematopoietic cells to produce CD4+ regulatory T cells
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• nave CD4+ T cells only produce one-third the amount of IL-2 as controls after in vitro stimulation
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• most mice have small or absent inguinal lymph nodes
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• nave CD4+ T cells stimulated in vitro through the TCR only have a third the proliferative response as control mice
• 66% fewer naive CD4+ T cells go into S-phase with 24 hours of TCR stimulation compared to controls
• the S-phase entry defect can be partly overcome by CD28 stimulation
• allogeneic proliferative response of nave CD4+ T cells is reduced 6-fold compared to controls
• the amount of IL-2 required for a half maximal proliferation response is 100-fold higher than in controls
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• CD4+ memory T cells are reduced by 3-fold in the spleen
• splenic CD8+ memory T cells are also reduced
• reconstitution of wild-type mice with both wild-type and mutant bone marrow demonstrate an intrinsic defect of mutant hematopoietic cells to produce CD4+ memory T cells
|
|
• there is a 75% decrease in the number of regulatory T cells found in the spleen, lymph node and thymus of mice
• reconstitution of wild-type mice with both wild-type and mutant bone marrow demonstrate an intrinsic defect of mutant hematopoietic cells to produce CD4+ regulatory T cells
|
|
• nave CD4+ T cells only produce one-third the amount of IL-2 as controls after in vitro stimulation
|
|
• nave CD4+ T cells stimulated in vitro through the TCR only have a third the proliferative response as control mice
• 66% fewer naive CD4+ T cells go into S-phase with 24 hours of TCR stimulation compared to controls
• the S-phase entry defect can be partly overcome by CD28 stimulation
• allogeneic proliferative response of nave CD4+ T cells is reduced 6-fold compared to controls
• the amount of IL-2 required for a half maximal proliferation response is 100-fold higher than in controls
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• there are slightly fewer thymic TCRbeta+NK1.1+ NKT cells
• the number of thymic CD1d-dependent NKT is also reduced
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• reconstitution of wild-type mice with mutant bone marrow leads to lower CD4+ memory T cells in the spleen and lymph nodes compared to controls reconstituted with wild-type bone marrow
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• reconstitution of wild-type mice with mutant bone marrow leads to 3-fold lower FoxP3+ CD4+ regulatory T cells in the spleen and lymph nodes compared to controls reconstituted with wild-type bone marrow
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• there are slightly fewer thymic TCRbeta+NK1.1+ NKT cells
• the number of thymic CD1d-dependent NKT is also reduced
|
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• reconstitution of wild-type mice with mutant bone marrow leads to lower CD4+ memory T cells in the spleen and lymph nodes compared to controls reconstituted with wild-type bone marrow
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• reconstitution of wild-type mice with mutant bone marrow leads to 3-fold lower FoxP3+ CD4+ regulatory T cells in the spleen and lymph nodes compared to controls reconstituted with wild-type bone marrow
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• after LPS injection
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• in bone marrow-derived macrophage
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• from bone marrow-derived macrophage stimulated with LPS
• from primary peritoneal macrophages from mice stimulated with LPS
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• after LPS injection
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• from bone marrow-derived macrophage stimulated with LPS
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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