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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Isl1tm2Sev
targeted mutation 2, Sylvia M Evans
MGI:3817457
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Isl1tm1(cre/Esr1*)Krc/Isl1tm2Sev involves: 129 * 129S1/Sv * 129X1/SvJ * C57BL/6 MGI:3817491
cn2
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Isl1tm2Sev/Isl1tm2Sev
H2az2Tg(Wnt1-cre)11Rth/H2az2+
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA MGI:3817490
cn3
Isl1tm2Sev/Isl1tm2.1Sev
Tg(Hoxb6-cre)#Mku/0
involves: 129S1/Sv * 129X1/SvJ * FVB/N MGI:5320482


Genotype
MGI:3817491
cn1
Allelic
Composition
Isl1tm1(cre/Esr1*)Krc/Isl1tm2Sev
Genetic
Background
involves: 129 * 129S1/Sv * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Isl1tm1(cre/Esr1*)Krc mutation (1 available); any Isl1 mutation (33 available)
Isl1tm2Sev mutation (1 available); any Isl1 mutation (33 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• induction of cre-recombinase expression at E11.5 leads to death of the animal during the perinatal period

nervous system
• the number of neurons expressing TrpVI and TrpM8 are markedly decreased in the dorsal root ganglion of E18.5 embryos that have had cre-recombinase induced at E11.5




Genotype
MGI:3817490
cn2
Allelic
Composition
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Isl1tm2Sev/Isl1tm2Sev
H2az2Tg(Wnt1-cre)11Rth/H2az2+
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1Sor mutation (8 available); any Gt(ROSA)26Sor mutation (935 available)
H2az2Tg(Wnt1-cre)11Rth mutation (2 available); any H2az2 mutation (26 available)
Isl1tm2Sev mutation (1 available); any Isl1 mutation (33 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• mice die within a few hours of birth

nervous system
• cutaneous branch of the ventral ramus is absent in E14.5 embryos
• innervation of the distal limbs at E14.5 confirmed a nearly complete loss of fine cutaneous sensory fibers with only a single sensory branch innervating one side of digits 1, 2 and 5 in both the forelimb and hindlimb
• there is an increased rate of apoptosis within the trigeminal ganglia of E11.5 and E12.5 embryos
• the dorsal root ganglion (DRG) of E12.5 embryos do not express Isl1 protein
• TrkA+ neurons are lower in number starting at E12.5 and by E14.5 are less than one-third of what is found in controls
• TrkB+ neurons are also lower in number starting at E12.5 and are markedly reduced at E14.5 and birth
• TrkC+ neurons do not appear until E12.5, a delay of two days compared to controls
• the DRG of E14.5 embryos is markedly smaller than controls with a smaller number neurons found within the ganglion
• an increased rate of apoptosis is noted in the E12.5 DRG

behavior/neurological
• mice have a reduced response to a mild noxious stimulus that was applied to the skin of the trunk or limbs




Genotype
MGI:5320482
cn3
Allelic
Composition
Isl1tm2Sev/Isl1tm2.1Sev
Tg(Hoxb6-cre)#Mku/0
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Isl1tm2.1Sev mutation (0 available); any Isl1 mutation (33 available)
Isl1tm2Sev mutation (1 available); any Isl1 mutation (33 available)
Tg(Hoxb6-cre)#Mku mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
limbs/digits/tail
• expression analysis indicates proximal patterning defects in the hindlimb buds
• variable severity of hindlimb digit loss
• almost half of mice display hindlimbs with only 3 digits
• less severely affected mice develop 4 hindlimb digits, lacking the posterior digit 5
• only a single zeugopodal element, likely corresponding to the tibia, is present in most mice with 1 - 3 digits on the hindlimb
• mice with 4 digits on the hindlimb show more variability in zeugopodal defects
• in severely affected mice

embryo
• expression analysis indicates proximal patterning defects in the hindlimb buds

skeleton
• patterning failure
• patterning failure





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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory