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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Klf5tm1Jaw
targeted mutation 1, Jeffrey A Whitsett
MGI:3806635
Summary 4 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Klf5tm1Jaw/Klf5tm1Jaw
Tg(SFTPC-rtTA)5Jaw/0
Tg(tetO-cre)1Jaw/0
involves: 129 * C57BL/6 MGI:3806650
cn2
Klf5tm1Jaw/Klf5tm1Jaw
Pgrtm2(cre)Lyd/Pgr+
involves: 129S1/Sv * 129X1/SvJ MGI:6870538
cn3
Klf4tm1Khk/Klf4tm1Khk
Klf5tm1Jaw/Klf5tm1Jaw
Tg(Pax6-cre,GFP)1Pgr/0
involves: 129S6/SvEvTac * FVB MGI:5644970
cn4
Klf5tm1Jaw/Klf5tm1Jaw
Tg(Pax6-cre,GFP)1Pgr/0
involves: FVB MGI:5644968


Genotype
MGI:3806650
cn1
Allelic
Composition
Klf5tm1Jaw/Klf5tm1Jaw
Tg(SFTPC-rtTA)5Jaw/0
Tg(tetO-cre)1Jaw/0
Genetic
Background
involves: 129 * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Klf5tm1Jaw mutation (1 available); any Klf5 mutation (37 available)
Tg(SFTPC-rtTA)5Jaw mutation (4 available)
Tg(tetO-cre)1Jaw mutation (5 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• doxycycline treated mice die shortly after birth due to respiratory distress

respiratory system
• in doxycycline treated mice, pulmonary mesenchyme is thickened and blood vessels are not found in close proximity to epithelial cells unlike in wild-type mice
• in doxycycline treated mice, lung maturation during saccule development is inhibited
• however, at E15.5 lung development is normal
• at birth, doxycycline treated mice exhibit hypercellular alveolar saccules without the dilated peripheral saccules found in wild-type mice
• saccules in doxycycline treated mice are thickened compared to in wild-type mice
• doxycycline treated mice exhibit thickened lung mesenchyme
• at birth, doxycycline treated mice exhibit a decrease in alveolar luminal area compared to wild-type mice
• at birth, doxycycline treated mice exhibit a decrease in squamous epithelial cells compared to in wild-type mice indicating a lack of alveolar type I cell differentiation
• however, pulmonary epithelial cell proliferation following dooxycycline treatment is normal
• at birth, doxycycline treated mice exhibit a decrease in squamous epithelial cells compared to in wild-type mice
• in doxycycline treated mice, type II cells lack lamellar structures found in wild-type cells
• doxycycline treated mice die shortly after birth due to respiratory distress
• unlike in wild-type mice, no surfactant secretion or Sftpb protein is detected in doxycycline treated mice




Genotype
MGI:6870538
cn2
Allelic
Composition
Klf5tm1Jaw/Klf5tm1Jaw
Pgrtm2(cre)Lyd/Pgr+
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Klf5tm1Jaw mutation (1 available); any Klf5 mutation (37 available)
Pgrtm2(cre)Lyd mutation (0 available); any Pgr mutation (73 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
• although stromal cells were able to initiate decidualization, the decidual response was severely impaired with smaller decidual sizes and no viable embryos present in implantation chambers on day 8 of pregnancy
• after oil-induced artificial decidualization, fold increase in uterine weight of infused-to-non-infused horns was significantly less than that in controls on day 8 of pseudopregnancy
• on day 5 of pregnancy, COX2 expression was absent in the uterine luminal epithelium but present in stromal cells at the blastocyst site, suggesting that poor embryo attachment might be due to aberrant COX2 expression
• although embryos did develop to blastocysts in uteri, most failed to implant or showed defective implantation
• blastocysts remained entrapped within the luminal epithelium past the window of implantation
• on day 5 of pregnancy, 8 of 11 females exhibited implantation sites but the number of implantation sites per female was significantly lower than that in control females
• on day 6, the uterine luminal epithelium around the implantation chamber failed to degenerate and show loss of E-cadherin, unlike in control uteri
• on day 7, implanted embryos were either very small or absorbed; some embryos were still surrounded by epithelial cells but showed signs of degeneration
• most implantation sites showed continued growth until day 8 but the number of sites on day 8 was similar to that on days 5 and 6
• on day 8, the weight of implantation sites was significantly lower than that in control females
• however, initiation of embryo-uterine interplay was not delayed
• uteri are largely unfavorable for normal implantation
• however, uterine responsiveness to estrogen and P4 was normal
• most females were found to be infertile
• after mating with wild-type males, only 2 of 10 plug-positive females produced pups
• 2 of 10 plug-positive females tested gave birth to only 2 and 3 pups/litter whereas control females delivered 8 pups/litter

embryo
• although stromal cells were able to initiate decidualization, the decidual response was severely impaired with smaller decidual sizes and no viable embryos present in implantation chambers on day 8 of pregnancy
• after oil-induced artificial decidualization, fold increase in uterine weight of infused-to-non-infused horns was significantly less than that in controls on day 8 of pseudopregnancy

homeostasis/metabolism
N
• females show normal serum estrogen (E2) and progesterone (P4) levels on day 4 of pregnancy




Genotype
MGI:5644970
cn3
Allelic
Composition
Klf4tm1Khk/Klf4tm1Khk
Klf5tm1Jaw/Klf5tm1Jaw
Tg(Pax6-cre,GFP)1Pgr/0
Genetic
Background
involves: 129S6/SvEvTac * FVB
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Klf4tm1Khk mutation (1 available); any Klf4 mutation (24 available)
Klf5tm1Jaw mutation (1 available); any Klf5 mutation (37 available)
Tg(Pax6-cre,GFP)1Pgr mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
vision/eye
• at 8 weeks of age, the mutant corneal stroma is thinner with relatively fewer keratocytes relative to wild-type and single Klf5 conditional knockout corneas
• mutant eyes fail to open as late as 35 weeks after birth, the latest stage examined
• co-ablation of Klf4 and Klf5 in the ocular surface results in more severe eyelid and corneal abnormalities than those in either single conditional knockout
• at 8 weeks of age, the mutant conjunctival epithelium is thinner than the wild-type or single Klf5 conditional knockout epithelium
• at 8 weeks of age, conjunctiva goblet cells are missing
• at 8 weeks of age, the mutant corneal epithelium is thinner than the wild-type and single Klf5 conditional knockout corneal epithelia, and remains fused to the eyelids




Genotype
MGI:5644968
cn4
Allelic
Composition
Klf5tm1Jaw/Klf5tm1Jaw
Tg(Pax6-cre,GFP)1Pgr/0
Genetic
Background
involves: FVB
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Klf5tm1Jaw mutation (1 available); any Klf5 mutation (37 available)
Tg(Pax6-cre,GFP)1Pgr mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
vision/eye
• at 8 weeks of age, mutant lacrimal glands display numerous infiltrating cells, indicating inflammation
• at 8 weeks of age, a thicker extra-ocular muscle is observed
• >80% of adult mutant eyes are small and contain a hypertrophic iris
• >80% of adult mutant eyes exhibit small pupils
• frequent iridocorneal fusion is observed
• frequent iridolenticular fusion is observed
• at 8 weeks of age, a rough and translucent cornea is observed
• mutant corneas are thicker than wild-type at P6, P11, P21 and at 10 weeks of age
• however, corneas appear normal at E13.5, E15.5 and E18.5
• mutant corneal stroma is relatively more intensely stained by PAS, suggesting increased levels of proteoglycans
• postnatal mutant corneal stroma is hypercellular
• at 8 weeks of age, central corneal stromal thickness and cell density are increased
• at 8 weeks of age
• mutant lenses appear spongy and deformed at P6, P11, P21 and at 10 weeks of age
• at E18.5 and P1, mutant lenses contain significantly fewer epithelial cells in the central anterior region than wild-type controls
• also, fewer and abnormally arranged nuclei are observed in the differentiating equatorial region
• although mutant primary lens fiber cells appear normal at E13.5 and E15.5, nuclei in E18.5 and P1 lens equatorial regions are disorganized, suggesting defects in secondary fiber formation
• >80% of postnatal mutant lenses are smaller than wild-type
• at 8 weeks of age, the enucleated mutant eyeballs are relatively smaller than wild-type
• small eyes are noted at P6, P11, P21 and at 10 weeks of age
• >80% of adult mutant eyes show a small eye phenotype
• however, early eye development is relatively normal
• at P21, mutant eyelashes are irregularly oriented and the surrounding fur is disorganized
• mutant eyelids are swollen and hypercellular and contain fewer irregularly spaced Meibomian gland orifices at the mucocutaneous junction
• the palpebral epidermis is thicker with 7-9 layers of keratinocytes relative to 2-3 in wild-type controls
• mutant eyelids contain significantly enlarged hair follicles and sebaceous glands
• however, no major differences are observed in eyelid morphology at P5, P8 and P11
• mutant eyelids are swollen at P6, P11, P21 and at 10 weeks of age
• at P21, mutant Meibomian glands exhibit disorganized, variably sized acini
• a Meibomian gland bud is noted at P6 suggesting timely induction, but appears disorganized; this feature becomes more prominent at P11 and P21
• at P21, the swollen eyelids are hypercellular and contain severely malformed Meibomian glands with disorganized acini
• while wild-type eyelids open at P12, mutant eyelids remain closed as late as P21
• small palpebral fissure at P21
• at P21, mutants display a >2-fold increase in epithelial cell proliferation in the eyelids, cornea, and conjunctiva, as determined by Ki67 staining
• Ki67-positive cells are significantly increased in mutant palpebral epidermis and eyelid hair follicles
• while Ki67-positive cells are restricted to basal epithelia in wild-type controls, they are also found in the spinous cell layers in the mutant cornea and conjunctiva
• at 8 weeks of age, mutant lacrimal glands display excessive vasculature and disrupted acinar organization, unlike wild-type controls
• at 8 weeks of age, mutant lacrimal glands display interspersed dark spots resembling necrotic spots
• at P21 and at 10 weeks, the mutant conjunctival epithelium appears rough and discontinuous, indicating a disrupted epithelial barrier
• at P21 and at 10 weeks, conjunctiva goblet cells are absent, unlike in wild-type controls
• at P21, the mutant palpebral conjunctiva is swollen and inflamed (reddish)
• at P21 and at 10 weeks, the mutant corneal epithelial basement membrane is thin and discontinuous, unlike in wild-type controls

cellular
• at 8 weeks of age, mutant lacrimal glands display interspersed dark spots resembling necrotic spots
• at P21, mutants display a >2-fold increase in epithelial cell proliferation in the eyelids, cornea, and conjunctiva, as determined by Ki67 staining
• Ki67-positive cells are significantly increased in mutant palpebral epidermis and eyelid hair follicles
• while Ki67-positive cells are restricted to basal epithelia in wild-type controls, they are also found in the spinous cell layers in the mutant cornea and conjunctiva

endocrine/exocrine glands
• at P21, mutant Meibomian glands exhibit disorganized, variably sized acini
• a Meibomian gland bud is noted at P6 suggesting timely induction, but appears disorganized; this feature becomes more prominent at P11 and P21
• at P21, the swollen eyelids are hypercellular and contain severely malformed Meibomian glands with disorganized acini
• at P21, mutant sebaceous glands are significantly enlarged
• at 8 weeks of age, mutant lacrimal glands display excessive vasculature and disrupted acinar organization, unlike wild-type controls
• at 8 weeks of age, mutant lacrimal glands display interspersed dark spots resembling necrotic spots
• at P21, an uneven lipid accumulation is noted in the Meibomian gland ducts
• at 8 weeks of age, mutant lacrimal glands display numerous infiltrating cells, indicating inflammation

immune system
• at 8 weeks of age, mutant lacrimal glands display numerous infiltrating cells, indicating inflammation

integument
• at P21, mutant Meibomian glands exhibit disorganized, variably sized acini
• a Meibomian gland bud is noted at P6 suggesting timely induction, but appears disorganized; this feature becomes more prominent at P11 and P21
• at P21, the swollen eyelids are hypercellular and contain severely malformed Meibomian glands with disorganized acini
• at P21, mutant sebaceous glands are significantly enlarged
• at P21, an uneven lipid accumulation is noted in the Meibomian gland ducts
• at P21, mutant eyelids contain significantly enlarged hair follicles

growth/size/body
• mutant eyelids are swollen at P6, P11, P21 and at 10 weeks of age

muscle
• at 8 weeks of age, a thicker extra-ocular muscle is observed

homeostasis/metabolism
• mutant eyelids are swollen at P6, P11, P21 and at 10 weeks of age

craniofacial
• mutant eyelids are swollen at P6, P11, P21 and at 10 weeks of age





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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory