Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ightm1Rbr mutation
(4 available);
any
Igh mutation
(43 available)
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hematopoietic system
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• about 0.4% of B cells in the spleen produce immunoglobulins with high affinity for hen egg lysozyme antigen (HEL)
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immune system
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• about 0.4% of B cells in the spleen produce immunoglobulins with high affinity for hen egg lysozyme antigen (HEL)
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ightm1Rbr mutation
(4 available);
any
Igh mutation
(43 available)
Tg(IgkHyHEL10)1Rbr mutation
(4 available)
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hematopoietic system
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• 40-60% of B cells in the spleen produce immunoglobulins that bind hen egg lysozyme antigen (HEL) with high affinity
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• the splenic B cells that bind HEL antigen are immature as indicated by the IgMhi IgDhi surface markers
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• all of the B cells found in the peritoneal cavity that bind HEL antigen are of the B-2 lineage as determined by B220hi CD5lowexpression
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• there are low levels of anti-HEL IgA in the sera of unimmunized mice
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• there are low levels of anti-HEL IgG1 in the sera of unimmunized mice
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• there are low levels of anti-HEL IgG2a in the sera of unimmunized mice
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• there are low levels of anti-HEL IgG2b in the sera of unimmunized mice
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• there are low levels of anti-HEL IgG3 in the sera of unimmunized mice
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• there are high levels of anti-HEL IgM in the sera of unimmunized mice
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immune system
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• 40-60% of B cells in the spleen produce immunoglobulins that bind hen egg lysozyme antigen (HEL) with high affinity
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• the splenic B cells that bind HEL antigen are immature as indicated by the IgMhi IgDhi surface markers
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• all of the B cells found in the peritoneal cavity that bind HEL antigen are of the B-2 lineage as determined by B220hi CD5lowexpression
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• there are low levels of anti-HEL IgA in the sera of unimmunized mice
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• there are low levels of anti-HEL IgG1 in the sera of unimmunized mice
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• there are low levels of anti-HEL IgG2a in the sera of unimmunized mice
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• there are low levels of anti-HEL IgG2b in the sera of unimmunized mice
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• there are low levels of anti-HEL IgG3 in the sera of unimmunized mice
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• there are high levels of anti-HEL IgM in the sera of unimmunized mice
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hematopoietic system
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• there is a 2-fold reduction in the number of HEL-specific B cells found in the spleen
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• HEL-specific B cells in the spleen have mostly have an immature phenotype
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• few HEL-specific B cells in the spleen are found in the follicular region or have surface markers indicative of follicular B cells
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• very few HEL-specific B cells in the spleen are found in the marginal zone or have surface markers indicative of the marginal zone phenotype
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immune system
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• there is a 2-fold reduction in the number of HEL-specific B cells found in the spleen
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• HEL-specific B cells in the spleen have mostly have an immature phenotype
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• few HEL-specific B cells in the spleen are found in the follicular region or have surface markers indicative of follicular B cells
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• very few HEL-specific B cells in the spleen are found in the marginal zone or have surface markers indicative of the marginal zone phenotype
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• B cells are anergic when stimulated with HEL antigen as determined by reduced antibody production, no upregulation of activation markers CD69 and CD86, and failure to increase size
• these anergic B cells have a short half-life of less than three days
• however, these anergic B cells can proliferate in response to BCR-independent activation
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gpr183tm1.2Rbr mutation
(0 available);
any
Gpr183 mutation
(23 available)
Ightm1Rbr mutation
(4 available);
any
Igh mutation
(43 available)
Tg(IgkHyHEL10)1Rbr mutation
(4 available)
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immune system
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• 5 days following immunization with sheep red blood cell, B cells are observed at reduced frequency in the spleen compared to in similarly treated Ightm1Rbr/Ightm1Rbr Tg(IgkHyHEL10)1Rbr mice
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• after activation with HEL-conjugated sheep red blood cell, fewer B cells acquired plasmablasts phenotype compared to in similarly treated Ightm1Rbr/Ightm1Rbr Tg(IgkHyHEL10)1Rbr mice
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• 1 day after activation with HEL-conjugated sheep red blood cell, B cells do not distribute uniformly along the B-T boundary in the spleen and often migrate deep into T cell areas unlike in similarly treated Ightm1Rbr/Ightm1Rbr Tg(IgkHyHEL10)1Rbr mice
• 3 days after activation with HEL-conjugated sheep red blood cell, B cells fail to move to the poles of the B cell follicles proximal to bridging and interfollicular regions and instead remain localized in central follicular areas proximal to the T cell zone unlike in similarly treated Ightm1Rbr/Ightm1Rbr Tg(IgkHyHEL10)1Rbr mice
• B cells fail to a robust extrafollicular plasmablast response due to reduced migration of responding B cells to the bridging channel and red pulp arrears unlike in Ightm1Rbr/Ightm1Rbr Tg(IgkHyHEL10)1Rbr mice
• however, expression of chemokine receptors is normal
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• following immunization with sheep red blood cell, B cell proliferation is delayed compared to in similarly treated Ightm1Rbr/Ightm1Rbr Tg(IgkHyHEL10)1Rbr mice
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• following activation with HEL-conjugated sheep red blood cell, anti-HEL IgG1 levels are reduced to 10% to 20% of that in similarly treated Ightm1Rbr/Ightm1Rbr Tg(IgkHyHEL10)1Rbr mice
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• following activation with HEL-conjugated sheep red blood cell, anti-HEL IgM levels are reduced to 10% to 20% of that in similarly treated Ightm1Rbr/Ightm1Rbr Tg(IgkHyHEL10)1Rbr mice
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hematopoietic system
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• 5 days following immunization with sheep red blood cell, B cells are observed at reduced frequency in the spleen compared to in similarly treated Ightm1Rbr/Ightm1Rbr Tg(IgkHyHEL10)1Rbr mice
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• after activation with HEL-conjugated sheep red blood cell, fewer B cells acquired plasmablasts phenotype compared to in similarly treated Ightm1Rbr/Ightm1Rbr Tg(IgkHyHEL10)1Rbr mice
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• 1 day after activation with HEL-conjugated sheep red blood cell, B cells do not distribute uniformly along the B-T boundary in the spleen and often migrate deep into T cell areas unlike in similarly treated Ightm1Rbr/Ightm1Rbr Tg(IgkHyHEL10)1Rbr mice
• 3 days after activation with HEL-conjugated sheep red blood cell, B cells fail to move to the poles of the B cell follicles proximal to bridging and interfollicular regions and instead remain localized in central follicular areas proximal to the T cell zone unlike in similarly treated Ightm1Rbr/Ightm1Rbr Tg(IgkHyHEL10)1Rbr mice
• B cells fail to a robust extrafollicular plasmablast response due to reduced migration of responding B cells to the bridging channel and red pulp arrears unlike in Ightm1Rbr/Ightm1Rbr Tg(IgkHyHEL10)1Rbr mice
• however, expression of chemokine receptors is normal
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• following immunization with sheep red blood cell, B cell proliferation is delayed compared to in similarly treated Ightm1Rbr/Ightm1Rbr Tg(IgkHyHEL10)1Rbr mice
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• following activation with HEL-conjugated sheep red blood cell, anti-HEL IgG1 levels are reduced to 10% to 20% of that in similarly treated Ightm1Rbr/Ightm1Rbr Tg(IgkHyHEL10)1Rbr mice
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• following activation with HEL-conjugated sheep red blood cell, anti-HEL IgM levels are reduced to 10% to 20% of that in similarly treated Ightm1Rbr/Ightm1Rbr Tg(IgkHyHEL10)1Rbr mice
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cellular
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• following immunization with sheep red blood cell, B cell proliferation is delayed compared to in similarly treated Ightm1Rbr/Ightm1Rbr Tg(IgkHyHEL10)1Rbr mice
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Analysis Tools