All Phenotypes Tg(Dct-lacZ)A12Jkn MGI Mouse

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Phenotypes associated with this allele

Allele Symbol
Allele Name
Allele ID

Tg(Dct-lacZ)A12Jkn
transgene insertion A12, Ian Jackson
MGI:3783632

Summary

23 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Ctnnb1^tm2Kem/Ctnnb1^tm2Kem Tg(Dct-lacZ)A12Jkn/0 Tg(Tyr-cre)1Lru/0	B6.Cg-Ctnnb1^tm2Kem Tg(Tyr-cre)1Lru Tg(Dct-lacZ)A12Jkn	MGI:6272344
cn2	Gt(ROSA)26Sor^{tm1(GNAQ*)Cvrk}/Gt(ROSA)26Sor⁺ Tg(Dct-lacZ)A12Jkn/0 Tg(Mitf-cre)7114Gsb/0	C3FeJ.Cg-Gt(ROSA)26Sor^{tm1(GNAQ*)Cvrk} Tg(Dct-lacZ)A12Jkn Tg(Mitf-cre)7114Gsb/Cvrk	MGI:5702881
cn3	Zeb2^tm1.1Yhi/Zeb2^tm1.1Yhi Tg(Dct-lacZ)A12Jkn/0 Tg(Tyr-cre)1Lru/Y	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA * DBA/2	MGI:6220636
cn4	Bcl2^tm1Sjk/Bcl2^tm1Sjk Tg(Dct-lacZ)A12Jkn/0	involves: 129S2/SvPas * C57BL/6 * C57BL/6J * CBA	MGI:6241340
cn5	Rest^tm1.1Yasu/Rest⁺ H2az2^{Tg(Wnt1-cre)11Rth}/H2az2⁺ Tg(Dct-lacZ)A12Jkn/0	involves: 129S4/SvJae * C57BL/6 * CBA	MGI:6241536
cn6	Rest^tm1.1Yasu/Rest^tm1.1Yasu H2az2^{Tg(Wnt1-cre)11Rth}/H2az2⁺ Tg(Dct-lacZ)A12Jkn/0	involves: 129S4/SvJae * C57BL/6 * CBA	MGI:6241535
cn7	Yy1^tm2.1Yshi/Yy1^tm2.1Yshi Tg(Dct-lacZ)A12Jkn/0 Tg(Tyr-cre)1Lru/Y	involves: 129S4/SvJae * C57BL/6 * CBA * DBA/2	MGI:6226060
cn8	Dct^tm1(cre)Bee/Dct^tm1(cre)Bee Tgfbr2^tm1Roes/Tgfbr2^tm1Roes Tg(Dct-lacZ)A12Jkn/0	involves: 129/Sv * C57BL/6 * C57BL/6J * CBA	MGI:6241339
cn9	Raph1^tm1.1Makr/Raph1^tm1.1Makr Tmem163^{Tg(ACTB-cre)2Mrt}/0 Tg(Dct-lacZ)A12Jkn/Tg(Dct-lacZ)A12Jkn	involves: C57BL/6 * C57BL/6NTac * CBA * FVB/N	MGI:5575559
cn10	Chek1^tm1Jmr/Chek1⁺ Tg(Dct-lacZ)A12Jkn/0 Tg(Tyr-cre)1Lru/Y	involves: C57BL/6 * CBA * DBA/2	MGI:6220869
cn11	Chek1^tm1Jmr/Chek1^tm1Jmr Tg(Dct-lacZ)A12Jkn/0 Tg(Tyr-cre)1Lru/Y	involves: C57BL/6 * CBA * DBA/2	MGI:6220868
cx12	Tg(Dct-lacZ)A12Jkn/0 Tg(Tyr-Ctnnb1/EGFP)#Lru/0	B6.Cg-Tg(Tyr-Ctnnb1/EGFP)#Lru Tg(Dct-lacZ)A12Jkn	MGI:6272590
cx13	Gli3^Mos1/Gli3⁺ Tg(Dct-lacZ)A12Jkn/0	involves: BALB/cJ * C57BL/6 * C57BL/6J * CBA	MGI:3797120
cx14	Gli3^Mos1/Gli3^Mos1 Tg(Dct-lacZ)A12Jkn/0	involves: BALB/cJ * C57BL/6 * C57BL/6J * CBA	MGI:3797121
cx15	Mitf^mi-bw/Mitf^mi-bw Tg(Dct-lacZ)A12Jkn/0	involves: C3H * C57BL/6 * C57BL/6J * CBA	MGI:5635983
cx16	Adamts9^Mhdaund3/Adamts9⁺ Tg(Dct-lacZ)A12Jkn/0	involves: C3HeB/FeJ * C57BL/6 * CBA	MGI:6163984
cx17	Rps20^Mhdadsk4/Rps20⁺ Tg(Dct-lacZ)A12Jkn/0	involves: C3HeB/FeJ * C57BL/6 * CBA	MGI:6259997
cx18	Rps19^Mhdadsk3/Rps19⁺ Tg(Dct-lacZ)A12Jkn/0	involves: C3HeB/FeJ * C57BL/6 * CBA	MGI:6259996
cx19	Adamts9^Mhdaund4/Adamts9⁺ Tg(Dct-lacZ)A12Jkn/0	involves: C3HeB/FeJ * C57BL/6 * CBA	MGI:6163981
cx20	Adam10^Pied/Adam10⁺ Hr^hr/Hr^hr Tg(Dct-lacZ)A12Jkn/0	involves: C3HeB/FeJ * C57BL/6 * CBA * HRA/SkhKcl	MGI:6163724
cx21	Fscn1^{Gt(OST124903)Lex}/Fscn1^{Gt(OST124903)Lex} Tg(Dct-lacZ)A12Jkn/0	involves: C57BL/6 * CBA	MGI:6209597
cx22	Fscn1^{Gt(OST124903)Lex}/Fscn1⁺ Tg(Dct-lacZ)A12Jkn/0	involves: C57BL/6 * CBA	MGI:6209603
cx23	Adamts20^bt-2H/Adamts20^bt-2H Tg(Dct-lacZ)A12Jkn/0	involves: C57BL/6 * CBA	MGI:3783650

Allelic Composition	Ctnnb1^tm2Kem/Ctnnb1^tm2Kem Tg(Dct-lacZ)A12Jkn/0 Tg(Tyr-cre)1Lru/0
Genetic Background	B6.Cg-Ctnnb1^tm2Kem Tg(Tyr-cre)1Lru Tg(Dct-lacZ)A12Jkn

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ctnnb1^tm2Kem mutation (1 available); any Ctnnb1 mutation (49 available)
Tg(Dct-lacZ)A12Jkn mutation (4 available)
Tg(Tyr-cre)1Lru mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

embryo

decreased melanoblast proliferation ( J:176245 )

• overall, melanoblasts exhibit a much lower level of BrdU incorporation in the epidermis (E12.5 to E14.5) and dermis (E14.5) relative to wild-type controls

• however, no differences in apoptosis, cell differentiation or fate are observed

abnormal melanoblast morphology ( J:176245 )

• in the trunk region, melanoblast number is normal at E10.5 but significantly lower than that in wild-type controls at each developmental stage from E12.5 to E15.5; this difference increases with time

• at E14.5, melanoblasts are less abundant than in mice hemizygous for the Tg(Tyr-Ctnnb1/EGFP)#Lru transgene, reflecting the differences in coat color observed after birth

• although melanoblast numbers are reduced in both the epidermis and dermis, the reduction is far greater in the epidermis than in the dermis

cellular

decreased melanoblast proliferation ( J:176245 )

• overall, melanoblasts exhibit a much lower level of BrdU incorporation in the epidermis (E12.5 to E14.5) and dermis (E14.5) relative to wild-type controls

• however, no differences in apoptosis, cell differentiation or fate are observed

nervous system

abnormal melanoblast morphology ( J:176245 )

• at E14.5, melanoblasts are less abundant than in mice hemizygous for the Tg(Tyr-Ctnnb1/EGFP)#Lru transgene, reflecting the differences in coat color observed after birth

• although melanoblast numbers are reduced in both the epidermis and dermis, the reduction is far greater in the epidermis than in the dermis

Allelic Composition	Gt(ROSA)26Sor^{tm1(GNAQ*)Cvrk}/Gt(ROSA)26Sor⁺ Tg(Dct-lacZ)A12Jkn/0 Tg(Mitf-cre)7114Gsb/0
Genetic Background	C3FeJ.Cg-Gt(ROSA)26Sor^{tm1(GNAQ*)Cvrk} Tg(Dct-lacZ)A12Jkn Tg(Mitf-cre)7114Gsb/Cvrk

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sor^{tm1(GNAQ*)Cvrk} mutation (0 available); any Gt(ROSA)26Sor mutation (942 available)
Tg(Dct-lacZ)A12Jkn mutation (4 available)
Tg(Mitf-cre)7114Gsb mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

pigmentation

pigmentation phenotype ( J:225597 )

N	• at 3 weeks of age, the number of lacZ+ melanocytes in tail scales is normal

abnormal melanosome morphology ( J:225597 )

• in older mice, melanin content in the tail scales is reduced compared to in control mice

Allelic Composition	Zeb2^tm1.1Yhi/Zeb2^tm1.1Yhi Tg(Dct-lacZ)A12Jkn/0 Tg(Tyr-cre)1Lru/Y
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA * DBA/2

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Dct-lacZ)A12Jkn mutation (4 available)
Tg(Tyr-cre)1Lru mutation (1 available)
Zeb2^tm1.1Yhi mutation (1 available); any Zeb2 mutation (76 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

pigmentation

abnormal hair follicle melanocyte morphology ( J:211299 )

• at P5.5, mice show significantly fewer pigmented or LacZ+ hair follicles than control mice; also, completely undifferentiated hair follicles that are neither pigmented nor LacZ+ are observed, unlike in control mice

• at P5.5, the total number of melanocytes (S100b+) per hair follicle is reduced by 40% relative to that in control mice

• at P5.5, mice exhibit formation of undifferentiated melanocytes in the bulge area of hair follicles, along with loss of MITF (the master regulator of melanocyte development) and upregulation of ZEB1 expression

• the number of MITF+ melanocytes is strongly reduced relative to the total number of melanocytes per hair follicle (S100b+ melanocytes), whereas the number of PAX3+ melanocytes is normal; also, MITF protein levels are reduced in MITF+ cells

• most hair follicles are negative for terminal melanocyte differentiation markers, such as TYRP1 and tyrosinase enzymatic activity

• mRNA expression of several melanocyte differentiation markers (Tyrp1,Tyr, Dct, Pmel, Mc1R and Mitf) is significantly down-regulated in skin at E15.5

abnormal melanosome morphology ( J:211299 )

• at P5.5, a few remaining melanosomes are visible in some hair follicles; however, these melanosomes are spherical with irregular borders, unlike the rod-shaped melanosomes of control hair follicles

absent hair follicle melanin granules ( J:211299 )

• at P5.5, ZEB2-stained sections revealed absence of melanin in most melanocytes of the bulb area

reduced hair shaft melanin granule number ( J:211299 )

• at P5.5, ZEB2-stained sections revealed absence of melanin in the hair shafts

integument

abnormal hair follicle melanocyte morphology ( J:211299 )

• at P5.5, the total number of melanocytes (S100b+) per hair follicle is reduced by 40% relative to that in control mice

• most hair follicles are negative for terminal melanocyte differentiation markers, such as TYRP1 and tyrosinase enzymatic activity

• mRNA expression of several melanocyte differentiation markers (Tyrp1,Tyr, Dct, Pmel, Mc1R and Mitf) is significantly down-regulated in skin at E15.5

absent hair follicle melanin granules ( J:211299 )

• at P5.5, ZEB2-stained sections revealed absence of melanin in most melanocytes of the bulb area

reduced hair shaft melanin granule number ( J:211299 )

• at P5.5, ZEB2-stained sections revealed absence of melanin in the hair shafts

abnormal hair follicle bulge morphology ( J:211299 )

• mice show a reduction in LacZ+ melanocyte stem cells in the bulge area relative to control mice, consistent with fewer melanoblasts found in the epidermis

embryo

abnormal melanoblast migration ( J:211299 )

• at E15.5, the number of LacZ+ melanoblasts in the epidermis of both the belly and the back is significantly lower than that in control embryos; however, 30% of them reach the dorsal area (back) at E15.5

• at P5.5, some hair follicles are still LacZ+ and/or (hypo-)pigmented, indicating that melanoblasts can migrate and populate the bulb area of the hair follicles

• the number of melanoblasts present in the dermis at E15.5 is normal

abnormal melanoblast morphology ( J:211299 )

• at E15.5, the number of LacZ+ melanoblasts is significantly reduced in both dorsal and ventral areas relative to control embryos; melanoblast numbers are reduced more on the belly (95%) than on the back (70%), consistent with a migration defect

• at E15.5, melanoblasts exhibit less cell protrusions than control cells

nervous system

abnormal melanoblast morphology ( J:211299 )

• at E15.5, melanoblasts exhibit less cell protrusions than control cells

cellular

abnormal melanoblast migration ( J:211299 )

• at P5.5, some hair follicles are still LacZ+ and/or (hypo-)pigmented, indicating that melanoblasts can migrate and populate the bulb area of the hair follicles

• the number of melanoblasts present in the dermis at E15.5 is normal

Allelic Composition	Bcl2^tm1Sjk/Bcl2^tm1Sjk Tg(Dct-lacZ)A12Jkn/0
Genetic Background	involves: 129S2/SvPas * C57BL/6 * C57BL/6J * CBA

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bcl2^tm1Sjk mutation (1 available); any Bcl2 mutation (41 available)
Tg(Dct-lacZ)A12Jkn mutation (4 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

embryo

abnormal melanoblast morphology ( J:157070 )

• mice show complete loss of LacZ+ melanoblasts in the bulge area at the end stage of hair follicle morphogenesis (i.e. stage 8, but not stage 6), indicating apoptosis of bulge melanocyte stem cells; in contrast, pigmented melanocytes are still maintained in the bulb at stage 8

• mice treated with an anti-TGF-beta blocking antibody during the process of stem cell entry to the dormant state (stage 5-8) exhibit increased survival (rescue) of bulge LacZ+ melanoblasts at P4, P6, and P8, whereas untreated mice or mice treated with a control antibody display virtual loss of LacZ+ melanoblasts from the bulge area by P8

nervous system

abnormal melanoblast morphology ( J:157070 )

Allelic Composition	Rest^tm1.1Yasu/Rest⁺ H2az2^{Tg(Wnt1-cre)11Rth}/H2az2⁺ Tg(Dct-lacZ)A12Jkn/0
Genetic Background	involves: 129S4/SvJae * C57BL/6 * CBA

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
H2az2^{Tg(Wnt1-cre)11Rth} mutation (2 available); any H2az2 mutation (26 available)
Rest^tm1.1Yasu mutation (1 available); any Rest mutation (95 available)
Tg(Dct-lacZ)A12Jkn mutation (4 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

embryo

abnormal melanoblast morphology ( J:230341 )

• adult mice show a marked reduction in the number of Dct-LacZ+ melanoblasts and/or melanocytes in the white spot of abdominal skin

nervous system

abnormal melanoblast morphology ( J:230341 )

• adult mice show a marked reduction in the number of Dct-LacZ+ melanoblasts and/or melanocytes in the white spot of abdominal skin

Allelic Composition	Rest^tm1.1Yasu/Rest^tm1.1Yasu H2az2^{Tg(Wnt1-cre)11Rth}/H2az2⁺ Tg(Dct-lacZ)A12Jkn/0
Genetic Background	involves: 129S4/SvJae * C57BL/6 * CBA

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

embryo

abnormal melanoblast morphology ( J:230341 )

• at E12.5 and E14.5, the number of Dct-LacZ+ cells in the belly region is significantly lower than that in control mice

• at E17.5, the distribution pattern of LacZ+ cells is altered in skin from the abdomen, but not from the back or head

nervous system

abnormal melanoblast morphology ( J:230341 )

• at E12.5 and E14.5, the number of Dct-LacZ+ cells in the belly region is significantly lower than that in control mice

• at E17.5, the distribution pattern of LacZ+ cells is altered in skin from the abdomen, but not from the back or head

Allelic Composition	Yy1^tm2.1Yshi/Yy1^tm2.1Yshi Tg(Dct-lacZ)A12Jkn/0 Tg(Tyr-cre)1Lru/Y
Genetic Background	involves: 129S4/SvJae * C57BL/6 * CBA * DBA/2

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Dct-lacZ)A12Jkn mutation (4 available)
Tg(Tyr-cre)1Lru mutation (1 available)
Yy1^tm2.1Yshi mutation (0 available); any Yy1 mutation (34 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

Absence of melanocytes or pigment in hair follicles of Yy1^tm2.1Yshi/Yy1^tm2.1Yshi Tg(Tyr-cre)1Lru/Y Tg(Dct-lacZ)A12Jkn/0 mice at the anagen phase of the second hair cycle

pigmentation

abnormal hair follicle melanocyte morphology ( J:185128 )

• mice show absence of differentiated melanocytes in hair follicles at the anagen phase of the second hair cycle (P38)

absent hair follicle melanin granules ( J:185128 )

• mice show absence of pigment in hair follicles at the anagen phase of the second hair cycle (P38)

decreased melanocyte number ( J:185128 )

• absence of differentiated melanocytes in hair follicles at P38

integument

abnormal hair follicle melanocyte morphology ( J:185128 )

• mice show absence of differentiated melanocytes in hair follicles at the anagen phase of the second hair cycle (P38)

absent hair follicle melanin granules ( J:185128 )

• mice show absence of pigment in hair follicles at the anagen phase of the second hair cycle (P38)

embryo

abnormal melanoblast morphology ( J:185128 )

• absence of Dct-LacZ+ melanocyte stem cells (melanoblasts) in hair follicles at P38

nervous system

abnormal melanoblast morphology ( J:185128 )

• absence of Dct-LacZ+ melanocyte stem cells (melanoblasts) in hair follicles at P38

Allelic Composition	Dct^tm1(cre)Bee/Dct^tm1(cre)Bee Tgfbr2^tm1Roes/Tgfbr2^tm1Roes Tg(Dct-lacZ)A12Jkn/0
Genetic Background	involves: 129/Sv * C57BL/6 * C57BL/6J * CBA

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dct^tm1(cre)Bee mutation (1 available); any Dct mutation (35 available)
Tg(Dct-lacZ)A12Jkn mutation (4 available)
Tgfbr2^tm1Roes mutation (2 available); any Tgfbr2 mutation (39 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

pigmentation

abnormal coat/hair pigmentation ( J:157070 )

• 73.3% of mice exhibit mild but accelerated hair graying within 10 months after birth relative to control mice; the frequency of gray hairs is variable depending on the individual mouse

• hair graying is likely caused by incomplete maintenance of melanocyte stem cells

abnormal hair follicle melanocyte morphology ( J:157070 )

• late anagen follicles exhibit LacZ+ dendritic and slightly pigmented melanocytes with large cell bodies in the bulge-subbulge area; in contrast, LacZ+ melanoblasts in the bulge area of control follicles are immature and small with minimal dendrites, typical features of melanocyte stem cells

• at 6 months of age, the frequency of hair follicles with large and dendritic melanocytes or pigmented melanocytes in the bulge area of anagen IV-VI follicles is significantly higher than that in control follicles

• some follicles show complete loss of Dct-lacZ+ cells in the bulge area, suggesting that melanocyte stem cells have prematurely differentiated and are eventually depleted from the stem cell niche

• melanocyte stem cells are lost in the bulge area of almost all follicles that produce white hair

• in contrast, the distribution of epidermal and dermal melanocytes in the pigmented junctional epithelium is normal

ectopic hair follicle melanin granules ( J:157070 )

• mice exhibit deposition of melanin pigment in the bulge area of midanagen hair follicles

pigment incontinence ( J:157070 )

• melanin incontinence in the dermal papillae of hair follicles occurs more frequently than in control follicles

ectopic melanocytes ( J:157070 )

• midanagen hair follicles begin to show ectopically pigmented melanocytes with dendritic morphologies starting from the second hair cycle

• ectopically differentiated melanocytes are preferentially seen in follicles that show pigmentation defects in the hair matrix

integument

abnormal coat/hair pigmentation ( J:157070 )

• 73.3% of mice exhibit mild but accelerated hair graying within 10 months after birth relative to control mice; the frequency of gray hairs is variable depending on the individual mouse

• hair graying is likely caused by incomplete maintenance of melanocyte stem cells

abnormal hair follicle melanocyte morphology ( J:157070 )

• some follicles show complete loss of Dct-lacZ+ cells in the bulge area, suggesting that melanocyte stem cells have prematurely differentiated and are eventually depleted from the stem cell niche

• melanocyte stem cells are lost in the bulge area of almost all follicles that produce white hair

• in contrast, the distribution of epidermal and dermal melanocytes in the pigmented junctional epithelium is normal

ectopic hair follicle melanin granules ( J:157070 )

• mice exhibit deposition of melanin pigment in the bulge area of midanagen hair follicles

pigment incontinence ( J:157070 )

• melanin incontinence in the dermal papillae of hair follicles occurs more frequently than in control follicles

Allelic Composition	Raph1^tm1.1Makr/Raph1^tm1.1Makr Tmem163^{Tg(ACTB-cre)2Mrt}/0 Tg(Dct-lacZ)A12Jkn/Tg(Dct-lacZ)A12Jkn
Genetic Background	involves: C57BL/6 * C57BL/6NTac * CBA * FVB/N

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Raph1^tm1.1Makr mutation (1 available); any Raph1 mutation (59 available)
Tg(Dct-lacZ)A12Jkn mutation (4 available)
Tmem163^{Tg(ACTB-cre)2Mrt} mutation (3 available); any Tmem163 mutation (35 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

pigmentation

belly spot ( J:208191 )

decreased melanocyte number ( J:208191 )

• in the trunk region at E14.5

growth/size/body

decreased body size ( J:208191 )

integument

belly spot ( J:208191 )

Allelic Composition	Chek1^tm1Jmr/Chek1⁺ Tg(Dct-lacZ)A12Jkn/0 Tg(Tyr-cre)1Lru/Y
Genetic Background	involves: C57BL/6 * CBA * DBA/2

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chek1^tm1Jmr mutation (1 available); any Chek1 mutation (41 available)
Tg(Dct-lacZ)A12Jkn mutation (4 available)
Tg(Tyr-cre)1Lru mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

pigmentation

pigmentation phenotype ( J:265850 )

N	• despite a slight reduction of melanoblast numbers at E13.5, adult mice exhibit normal coat pigmentation relative to control mice

embryo

abnormal melanoblast morphology ( J:265850 )

• at E13.5, a slight but significant reduction of melanoblast numbers is noted in the head, dorsal, and ventral regions relative to control embryos

nervous system

abnormal melanoblast morphology ( J:265850 )

• at E13.5, a slight but significant reduction of melanoblast numbers is noted in the head, dorsal, and ventral regions relative to control embryos

Allelic Composition	Chek1^tm1Jmr/Chek1^tm1Jmr Tg(Dct-lacZ)A12Jkn/0 Tg(Tyr-cre)1Lru/Y
Genetic Background	involves: C57BL/6 * CBA * DBA/2

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

embryo

abnormal melanoblast morphology ( J:265850 )

• significantly fewer beta-gal+ melanoblasts are noted in the head and trunk regions at E12.5, and by E13.5, melanoblasts are completely lost even from the pinna, vibrissae, and around the eyes

• many cleaved caspase 3+ melanoblasts are observed in the head and trunk regions, unlike in control embryos, suggesting increased melanoblast apoptosis

• however, the number and distribution of beta-gal+ melanoblasts is normal at E10.5 and E11.5

nervous system

abnormal melanoblast morphology ( J:265850 )

• significantly fewer beta-gal+ melanoblasts are noted in the head and trunk regions at E12.5, and by E13.5, melanoblasts are completely lost even from the pinna, vibrissae, and around the eyes

• many cleaved caspase 3+ melanoblasts are observed in the head and trunk regions, unlike in control embryos, suggesting increased melanoblast apoptosis

• however, the number and distribution of beta-gal+ melanoblasts is normal at E10.5 and E11.5

cellular

abnormal DNA replication ( J:265850 )

• many gamma-H2AX+ melanoblasts are noted in the head and trunk regions, unlike in control embryos, suggesting that aberrant DNA replication leads to spontaneous DNA damage and ultimately cell death

homeostasis/metabolism

abnormal DNA replication ( J:265850 )

Allelic Composition	Tg(Dct-lacZ)A12Jkn/0 Tg(Tyr-Ctnnb1/EGFP)#Lru/0
Genetic Background	B6.Cg-Tg(Tyr-Ctnnb1/EGFP)#Lru Tg(Dct-lacZ)A12Jkn

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Dct-lacZ)A12Jkn mutation (4 available)
Tg(Tyr-Ctnnb1/EGFP)#Lru mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

embryo

decreased melanoblast proliferation ( J:176245 )

• overall, melanoblasts exhibit a lower level of BrdU incorporation in the epidermis (E12.5 to E14.5) and dermis (E13.5) relative to wild-type controls

• however, no differences in apoptosis, cell differentiation or fate are observed

abnormal melanoblast morphology ( J:176245 )

• in the trunk region, melanoblast number is normal at E10.5 but significantly lower than that in wild-type controls at each developmental stage from E11.5 to E15.5; this difference increases with time

• although melanoblast numbers are reduced in both the epidermis and dermis, the reduction is far greater in the epidermis than in the dermis

cellular

decreased melanoblast proliferation ( J:176245 )

• overall, melanoblasts exhibit a lower level of BrdU incorporation in the epidermis (E12.5 to E14.5) and dermis (E13.5) relative to wild-type controls

• however, no differences in apoptosis, cell differentiation or fate are observed

nervous system

abnormal melanoblast morphology ( J:176245 )

• although melanoblast numbers are reduced in both the epidermis and dermis, the reduction is far greater in the epidermis than in the dermis

Allelic Composition	Gli3^Mos1/Gli3⁺ Tg(Dct-lacZ)A12Jkn/0
Genetic Background	involves: BALB/cJ * C57BL/6 * C57BL/6J * CBA

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gli3^Mos1 mutation (0 available); any Gli3 mutation (80 available)
Tg(Dct-lacZ)A12Jkn mutation (4 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

embryo

abnormal melanoblast morphology ( J:136642 )

• at E16, mice exhibit a small ventral patch devoid of melanoblasts in the mid-trunk unlike in wild-type mice

nervous system

abnormal melanoblast morphology ( J:136642 )

• at E16, mice exhibit a small ventral patch devoid of melanoblasts in the mid-trunk unlike in wild-type mice

Allelic Composition	Gli3^Mos1/Gli3^Mos1 Tg(Dct-lacZ)A12Jkn/0
Genetic Background	involves: BALB/cJ * C57BL/6 * C57BL/6J * CBA

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gli3^Mos1 mutation (0 available); any Gli3 mutation (80 available)
Tg(Dct-lacZ)A12Jkn mutation (4 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

abnormal melanoblast morphology ( J:136642 )

• at E11.5, E14 and E16, mice exhibit fewer melanoblasts in the mid-trunk compared to in wild-type mice

abnormal telencephalon development ( J:136642 )

• the loss of transgene expression in the dorsal telecephalon is attributed to telecephalon ventralization

embryo

abnormal melanoblast morphology ( J:136642 )

• at E11.5, E14 and E16, mice exhibit fewer melanoblasts in the mid-trunk compared to in wild-type mice

Allelic Composition	Mitf^mi-bw/Mitf^mi-bw Tg(Dct-lacZ)A12Jkn/0
Genetic Background	involves: C3H * C57BL/6 * C57BL/6J * CBA

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mitf^mi-bw mutation (3 available); any Mitf mutation (72 available)
Tg(Dct-lacZ)A12Jkn mutation (4 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

pigmentation

absent hair follicle melanin granules ( J:213982 )

• lack of melanin

absent coat pigmentation ( J:213982 )

• white coat color, however eyes are black

decreased melanocyte number ( J:213982 )

• no melanocytes are seen in the hair follicles

embryo

abnormal melanoblast migration ( J:213982 )

• in neural tube cultures, melanoblasts are unable to migrate out from the neural tube

abnormal melanoblast morphology ( J:213982 )

• decrease in the number of melanoblasts between E12.5 and E13.5, with absence of melanoblasts in the trunk region at E13.5

• increase in melanoblast apoptosis at E12.5

integument

absent hair follicle melanin granules ( J:213982 )

• lack of melanin

absent coat pigmentation ( J:213982 )

• white coat color, however eyes are black

nervous system

abnormal melanoblast morphology ( J:213982 )

• decrease in the number of melanoblasts between E12.5 and E13.5, with absence of melanoblasts in the trunk region at E13.5

• increase in melanoblast apoptosis at E12.5

cellular

abnormal melanoblast migration ( J:213982 )

• in neural tube cultures, melanoblasts are unable to migrate out from the neural tube

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
Waardenburg syndrome type 2A		DOID:0110950	OMIM:193510	J:213982

Allelic Composition	Adamts9^Mhdaund3/Adamts9⁺ Tg(Dct-lacZ)A12Jkn/0
Genetic Background	involves: C3HeB/FeJ * C57BL/6 * CBA

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Adamts9^Mhdaund3 mutation (1 available); any Adamts9 mutation (90 available)
Tg(Dct-lacZ)A12Jkn mutation (4 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

pigmentation

abnormal epidermal pigmentation ( J:262498 )