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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Tg(Stra8-icre)1Reb
transgene insertion 1, Robert E Braun
MGI:3779079
Summary 21 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Hellstm1c(EUCOMM)Wtsi/Hellstm1d(EUCOMM)Wtsi
Tg(Stra8-icre)1Reb/0
(B6(Cg)-Hellstm1c(EUCOMM)Wtsi/BakrJ x B6.Cg-Hellstm1d(EUCOMM)Wtsi Tg(Stra8-icre)1Reb/Bakr)F1 MGI:6389564
cn2
Tg(Stra8-icre)1Reb/0
Upf3atm1.1Wilk/Upf3atm1.1Wilk
involves: 129 * 129S4/SvJaeSor * C57BL/6 * FVB/NJ MGI:6117761
cn3
Tg(Stra8-icre)1Reb/0
Upf3atm1.1Wilk/Upf3a+
involves: 129 * 129S4/SvJaeSor * C57BL/6 * FVB/NJ MGI:6117762
cn4
Cdk1tm3Kald/Cdk1+
Tg(Stra8-icre)1Reb/0
involves: 129 * C57BL/6 * FVB/NJ MGI:6201526
cn5
Fbxo43tm1.1Kald/Fbxo43tm1.1Kald
Tg(Stra8-icre)1Reb/0
involves: 129 * C57BL/6 * FVB/NJ * SJL MGI:6201523
cn6
Piwil2tm2.1Doca/Piwil2tm2.1Doca
Tg(Stra8-icre)1Reb/0
involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6J * FVB/NJ MGI:5521297
cn7
Piwil2tm1.1Doca/Piwil2tm2.1Doca
Tg(Stra8-icre)1Reb/0
involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6J * FVB/NJ MGI:5521299
cn8
Kdm6btm1.1Iwam/Kdm6btm1.2Iwam
Tg(Stra8-icre)1Reb/0
involves: 129P2/OlaHsd * C57BL/6J * FVB/NJ MGI:5551619
cn9
Rxfp2tm1Aia/Rxfp2tm1c(EUCOMM)Wtsi
Tg(Stra8-icre)1Reb/0
involves: 129S7/SvEvBrd * C57BL/6N * FVB/NJ MGI:5473319
cn10
Ppp1cctm1Lex/Ppp1cctm1Lex
Tg(Stra8-icre)1Reb/0
involves: 129S/SvEvBrd * FVB/NJ MGI:5559510
cn11
Rdh10tm1c(KOMP)Wtsi/Rdh10tm1c(KOMP)Wtsi
Tg(Amh-cre)8815Reb/0
Tg(RARE-Hspa1b/lacZ)12Jrt/0
Tg(Stra8-icre)1Reb/0
involves: C57BL/6 * C57BL/6N * CD-1 * FVB/N * SJL MGI:5475537
cn12
Rdh10tm1c(KOMP)Wtsi/Rdh10tm1c(KOMP)Wtsi
Tg(Stra8-icre)1Reb/0
involves: C57BL/6 * C57BL/6N * FVB/NJ * SJL MGI:5475535
cn13
Rdh10tm1c(KOMP)Wtsi/Rdh10tm1c(KOMP)Wtsi
Tg(Amh-cre)8815Reb/0
Tg(Stra8-icre)1Reb/0
involves: C57BL/6 * C57BL/6N * FVB/N * SJL MGI:5475536
cn14
Ranbp9tm1Wyan/Ranbp9tm1Wyan
Tg(Stra8-icre)1Reb/0
involves: C57BL/6J * FVB/NJ MGI:5702983
cn15
Tdrd5tm1c(EUCOMM)Hmgu/Tdrd5tm1c(EUCOMM)Hmgu
Tg(Stra8-icre)1Reb/0
involves: C57BL/6N * FVB/NJ MGI:6156877
cn16
Crisp2tm1c(KOMP)Mbp/Crisp2tm1c(KOMP)Mbp
Tg(Stra8-icre)1Reb/0
involves: C57BL/6N * FVB/NJ MGI:6369095
cn17
Katnal2tm1c(EUCOMM)Wtsi/Katnal2tm1c(EUCOMM)Wtsi
Tg(Stra8-icre)1Reb/0
involves: C57BL/6N * FVB/NJ MGI:6274241
cn18
Tg(RNU6-RNAi:Rhox3)2Wilk/0
Tg(Stra8-icre)1Reb/0
involves: FVB/NJ MGI:5763275
cn19
Tg(Stra8-icre)1Reb/0
Zscan21tm1.1Jiwa/Zscan21tm1.1Jiwa
involves: FVB/NJ MGI:5904161
cn20
Rhoxtm1Wilk/Rhoxtm1Wilk
Tg(RNU6-RNAi:Rhox3)2Wilk/0
Tg(Stra8-icre)1Reb/0
involves: FVB/NJ MGI:5763277
tg21
Tg(Stra8-icre)1Reb/0 involves: FVB MGI:3850155


Genotype
MGI:6389564
cn1
Allelic
Composition
Hellstm1c(EUCOMM)Wtsi/Hellstm1d(EUCOMM)Wtsi
Tg(Stra8-icre)1Reb/0
Genetic
Background
(B6(Cg)-Hellstm1c(EUCOMM)Wtsi/BakrJ x B6.Cg-Hellstm1d(EUCOMM)Wtsi Tg(Stra8-icre)1Reb/Bakr)F1
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hellstm1c(EUCOMM)Wtsi mutation (1 available); any Hells mutation (18 available)
Hellstm1d(EUCOMM)Wtsi mutation (0 available); any Hells mutation (18 available)
Tg(Stra8-icre)1Reb mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• At 12 days of age spermatocytes show a decrease in epigenomic modification at recombination hotspots, H3K4me3 and chromatin accessibility sites at recombination hotspots are decreased

endocrine/exocrine glands

reproductive system
• At 12 days of age spermatocytes show a decrease in epigenomic modification at recombination hotspots, H3K4me3 and chromatin accessibility sites at recombination hotspots are decreased
• Incomplete DNA repair at double stranded breaks in pachytene spermatocytes and the genomic locations of the double stranded breaks is abnormal
• arrest at the late-zygotene to early pachytene stage, with an increased proportion of leptotene and zygotene and absence of normal pachytene and diplotene spermatocytes, with the pachytene cells having H2AX phosphorylation and incomplete synapsis
• these males fail to sire offspring




Genotype
MGI:6117761
cn2
Allelic
Composition
Tg(Stra8-icre)1Reb/0
Upf3atm1.1Wilk/Upf3atm1.1Wilk
Genetic
Background
involves: 129 * 129S4/SvJaeSor * C57BL/6 * FVB/NJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Stra8-icre)1Reb mutation (2 available)
Upf3atm1.1Wilk mutation (0 available); any Upf3a mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
N
• number of apoptotic testicular cells at age 1 and 2 weeks
• reduced number of cells expressing spermatid-specific markers Tp2 and Prm2 amongst dissociated cells from purified seminiferous tubules
• reduced fraction of seminiferous tubules containing elongating spermatids
• 6-fold fewer pachytene spermatocytes compared to wild-type
• significant reduction in pachytene spermatocytes amongst dissociated cells from purified seminiferous tubules
• reduced number of cells expressing spermatocyte-specific markers Spo11 and Sycp3 amongst dissociated cells from purified seminiferous tubules
• reduced number of cells expressing spermatocyte-specific markers Stra8, Spo11 and Sycp3 amongst FACS sorted 4N spermatocytes
• reduced number of cells expressing germ cell marker Ldhc amongst dissociated cells from purified seminiferous tubules
• around 6-fold fewer round and elongated spermatids than wild-type
• 6-fold fewer pachytene spermatocytes compared to wild-type
• significant reduction in pachytene spermatocytes amongst dissociated cells from purified seminiferous tubules
• reduced fraction of seminiferous tubules containing elongating spermatids
• reduced number of cells expressing Ldhc (germ cell marker), Spo11 and Sycp3 (spermatocyte-specific markers), and Tp2 and Prm2 (spermatid-specific markers) amongst dissociated cells from purified seminiferous tubules
• normal number of leptotene/zygotene spermatocytes
• normal number of spermatogonia at age 1 and 2 weeks
• normal number of apoptotic testicular cells at age 1 and 2 weeks
• sperm count reduced 10-fold to around 10% of wild-type
• reduced expression of NMD (nonsense-mediated decay) target transcripts Atf4, Cdh11, Ern2, Smad5 and Smad7 in FACS sorted spermatocytes from 4-week old mice
• around 3-fold increase in apoptotic male germ cells per tubule at age 4 weeks

growth/size/body
N
• body weight and overall morphology

reproductive system
• reduced number of cells expressing spermatid-specific markers Tp2 and Prm2 amongst dissociated cells from purified seminiferous tubules
• reduced fraction of seminiferous tubules containing elongating spermatids
• 6-fold fewer pachytene spermatocytes compared to wild-type
• significant reduction in pachytene spermatocytes amongst dissociated cells from purified seminiferous tubules
• reduced number of cells expressing spermatocyte-specific markers Spo11 and Sycp3 amongst dissociated cells from purified seminiferous tubules
• reduced number of cells expressing spermatocyte-specific markers Stra8, Spo11 and Sycp3 amongst FACS sorted 4N spermatocytes
• reduced number of cells expressing germ cell marker Ldhc amongst dissociated cells from purified seminiferous tubules
• around 6-fold fewer round and elongated spermatids than wild-type
• 6-fold fewer pachytene spermatocytes compared to wild-type
• significant reduction in pachytene spermatocytes amongst dissociated cells from purified seminiferous tubules
• reduced fraction of seminiferous tubules containing elongating spermatids
• reduced number of cells expressing Ldhc (germ cell marker), Spo11 and Sycp3 (spermatocyte-specific markers), and Tp2 and Prm2 (spermatid-specific markers) amongst dissociated cells from purified seminiferous tubules
• normal number of leptotene/zygotene spermatocytes
• normal number of spermatogonia at age 1 and 2 weeks
• normal number of apoptotic testicular cells at age 1 and 2 weeks
• sperm count reduced 10-fold to around 10% of wild-type
• around 3-fold increase in apoptotic male germ cells per tubule at age 4 weeks
• significant reduction in pachytene spermatocytes amongst dissociated cells from purified seminiferous tubules
• 6-fold fewer pachytene spermatocytes compared to wild-type




Genotype
MGI:6117762
cn3
Allelic
Composition
Tg(Stra8-icre)1Reb/0
Upf3atm1.1Wilk/Upf3a+
Genetic
Background
involves: 129 * 129S4/SvJaeSor * C57BL/6 * FVB/NJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Stra8-icre)1Reb mutation (2 available)
Upf3atm1.1Wilk mutation (0 available); any Upf3a mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• reduced number of pachytene spermatocytes amongst FACS sorted cells at age 4 weeks
• reduced number of cells expressing spermatocyte-specific markers amongst purified spermatocytes
• reduced number of pachytene spermatocytes and postmeiotic germ cells amongst FACS sorted cells at age 4 weeks
• reduced number of cells expressing spermatocyte-specific markers amongst purified spermatocytes
• reduced number of cells expressing spermatogenic markers at age 4 weeks

reproductive system
• reduced number of pachytene spermatocytes amongst FACS sorted cells at age 4 weeks
• reduced number of cells expressing spermatocyte-specific markers amongst purified spermatocytes
• reduced number of pachytene spermatocytes and postmeiotic germ cells amongst FACS sorted cells at age 4 weeks
• reduced number of cells expressing spermatocyte-specific markers amongst purified spermatocytes
• reduced number of cells expressing spermatogenic markers at age 4 weeks




Genotype
MGI:6201526
cn4
Allelic
Composition
Cdk1tm3Kald/Cdk1+
Tg(Stra8-icre)1Reb/0
Genetic
Background
involves: 129 * C57BL/6 * FVB/NJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdk1tm3Kald mutation (0 available); any Cdk1 mutation (16 available)
Tg(Stra8-icre)1Reb mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
• tubules contain only Sertoli cells with only rare spermatogonia

endocrine/exocrine glands

cellular
• tubules contain only Sertoli cells with only rare spermatogonia




Genotype
MGI:6201523
cn5
Allelic
Composition
Fbxo43tm1.1Kald/Fbxo43tm1.1Kald
Tg(Stra8-icre)1Reb/0
Genetic
Background
involves: 129 * C57BL/6 * FVB/NJ * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Fbxo43tm1.1Kald mutation (0 available); any Fbxo43 mutation (17 available)
Tg(Stra8-icre)1Reb mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
• at stage XII (pachytene/diplotene)
• in prophase in meiosis I

cellular
• at stage XII (pachytene/diplotene)




Genotype
MGI:5521297
cn6
Allelic
Composition
Piwil2tm2.1Doca/Piwil2tm2.1Doca
Tg(Stra8-icre)1Reb/0
Genetic
Background
involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6J * FVB/NJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Piwil2tm2.1Doca mutation (1 available); any Piwil2 mutation (40 available)
Tg(Stra8-icre)1Reb mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
• especially in meiotic cells

cellular
• round spermatids fail to exhibit L1 transposon silencing unlike wild-type cells
• especially in meiotic cells

endocrine/exocrine glands




Genotype
MGI:5521299
cn7
Allelic
Composition
Piwil2tm1.1Doca/Piwil2tm2.1Doca
Tg(Stra8-icre)1Reb/0
Genetic
Background
involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6J * FVB/NJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Piwil2tm1.1Doca mutation (1 available); any Piwil2 mutation (40 available)
Piwil2tm2.1Doca mutation (1 available); any Piwil2 mutation (40 available)
Tg(Stra8-icre)1Reb mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
• especially in meiotic cells

cellular
• round spermatids fail to exhibit L1 transposon silencing unlike wild-type cells
• especially in meiotic cells

endocrine/exocrine glands




Genotype
MGI:5551619
cn8
Allelic
Composition
Kdm6btm1.1Iwam/Kdm6btm1.2Iwam
Tg(Stra8-icre)1Reb/0
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6J * FVB/NJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Kdm6btm1.1Iwam mutation (0 available); any Kdm6b mutation (45 available)
Kdm6btm1.2Iwam mutation (0 available); any Kdm6b mutation (45 available)
Tg(Stra8-icre)1Reb mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype

Enlarged testes in Kdm6btm1.1Iwam/Kdm6btm1.2Iwam Tg(Stra8-icre)1Reb/0 males

cellular
• increased Asingle spermatogonia in seminiferous tubules
• frequent spermatogonial dedifferentiation

reproductive system
• increased Asingle spermatogonia in seminiferous tubules
• frequent spermatogonial dedifferentiation
• male mice sire pups much longer without a change in letter size compared with wild-type mice
• however, litter production rate is normal

endocrine/exocrine glands




Genotype
MGI:5473319
cn9
Allelic
Composition
Rxfp2tm1Aia/Rxfp2tm1c(EUCOMM)Wtsi
Tg(Stra8-icre)1Reb/0
Genetic
Background
involves: 129S7/SvEvBrd * C57BL/6N * FVB/NJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rxfp2tm1Aia mutation (0 available); any Rxfp2 mutation (31 available)
Rxfp2tm1c(EUCOMM)Wtsi mutation (0 available); any Rxfp2 mutation (31 available)
Tg(Stra8-icre)1Reb mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
N
• mice exhibit normal reproductive organ abnormalities




Genotype
MGI:5559510
cn10
Allelic
Composition
Ppp1cctm1Lex/Ppp1cctm1Lex
Tg(Stra8-icre)1Reb/0
Genetic
Background
involves: 129S/SvEvBrd * FVB/NJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ppp1cctm1Lex mutation (0 available); any Ppp1cc mutation (30 available)
Tg(Stra8-icre)1Reb mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
N
• female mice exhibit normal fertility
• a few tubules contain spermatozoa in their lumen
• between the midpiece and principal pieces

cellular
• a few tubules contain spermatozoa in their lumen
• between the midpiece and principal pieces




Genotype
MGI:5475537
cn11
Allelic
Composition
Rdh10tm1c(KOMP)Wtsi/Rdh10tm1c(KOMP)Wtsi
Tg(Amh-cre)8815Reb/0
Tg(RARE-Hspa1b/lacZ)12Jrt/0
Tg(Stra8-icre)1Reb/0
Genetic
Background
involves: C57BL/6 * C57BL/6N * CD-1 * FVB/N * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rdh10tm1c(KOMP)Wtsi mutation (0 available); any Rdh10 mutation (0 available)
Tg(Amh-cre)8815Reb mutation (1 available)
Tg(RARE-Hspa1b/lacZ)12Jrt mutation (2 available)
Tg(Stra8-icre)1Reb mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system

endocrine/exocrine glands




Genotype
MGI:5475535
cn12
Allelic
Composition
Rdh10tm1c(KOMP)Wtsi/Rdh10tm1c(KOMP)Wtsi
Tg(Stra8-icre)1Reb/0
Genetic
Background
involves: C57BL/6 * C57BL/6N * FVB/NJ * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rdh10tm1c(KOMP)Wtsi mutation (0 available); any Rdh10 mutation (0 available)
Tg(Stra8-icre)1Reb mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
N
• testes weight and morphology are indistinguishable from controls at 2 and 3 weeks of age




Genotype
MGI:5475536
cn13
Allelic
Composition
Rdh10tm1c(KOMP)Wtsi/Rdh10tm1c(KOMP)Wtsi
Tg(Amh-cre)8815Reb/0
Tg(Stra8-icre)1Reb/0
Genetic
Background
involves: C57BL/6 * C57BL/6N * FVB/N * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rdh10tm1c(KOMP)Wtsi mutation (0 available); any Rdh10 mutation (0 available)
Tg(Amh-cre)8815Reb mutation (1 available)
Tg(Stra8-icre)1Reb mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• block of differentiation of Aal spermatogonia into A1 spermatogonia at 2 and 3 weeks of age

reproductive system
• block of differentiation of Aal spermatogonia into A1 spermatogonia at 2 and 3 weeks of age
• most tubules are devoid of meiotic cells at 2 and 3 weeks of age, retaining only undifferentiated spermatogonia-like cells and Sertoli cells
• however, by 9 weeks of age, tubules contain the expected spermatogonia, spermatocytes, and spermatids
• over 85% of tubules are degenerated at 2 and 3 weeks of age
• at 2 and 3 weeks of age
• dramatically lower at 2 and 3 weeks of age compared to wild-type controls and homozygous mice carrying only one of the cre transgenes
• most tubules are devoid of meiotic cells at 2 and 3 weeks of age, retaining only undifferentiated spermatogonia-like cells and Sertoli cells
• treatment with retinoic acid enhances synchrony of spermatogenesis
• however, by 9 weeks of age, spermatogenesis is similar to controls
• in adults only 1 of 48 males shows spermatogenesis with an altered stage frequency
• fail to initiate meiosis at 2 and 3 weeks of age
• numerous degenerated germ cells are present in the testes at 2 and 3 weeks of age
• males under 7 weeks of age are infertile or subfertile
• however, mice over 9 weeks of age are fertile and produce litters similar in size to controls

endocrine/exocrine glands
• most tubules are devoid of meiotic cells at 2 and 3 weeks of age, retaining only undifferentiated spermatogonia-like cells and Sertoli cells
• however, by 9 weeks of age, tubules contain the expected spermatogonia, spermatocytes, and spermatids
• over 85% of tubules are degenerated at 2 and 3 weeks of age
• at 2 and 3 weeks of age
• dramatically lower at 2 and 3 weeks of age compared to wild-type controls and homozygous mice carrying only one of the cre transgenes




Genotype
MGI:5702983
cn14
Allelic
Composition
Ranbp9tm1Wyan/Ranbp9tm1Wyan
Tg(Stra8-icre)1Reb/0
Genetic
Background
involves: C57BL/6J * FVB/NJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ranbp9tm1Wyan mutation (0 available); any Ranbp9 mutation (29 available)
Tg(Stra8-icre)1Reb mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
• epididymal sperm shows a variety of morphological abnormalities in the flagellum including coiled or headless flagellum
• epididymal sperm shows a variety of morphological abnormalities in the sperm head like crooked, round, or bent heads
• seminiferous epithelium is thinner in 3 month old testes and contains numerous vacuoles, indicative of germ cell depletion
• presence of numerous degenerating/degenerated spermatids, or even spermatocytes in the epididymis; this enhanced depletion of spermatocytes and spermatids and the production of deformed spermatozoa are seen only in mice older than 2 months and no differences are seen during the first wave of spermatogenesis
• proportional increase of meiotic cells (i.e.spermatocytes) and decrease in haploid cells (spermatids) despite lowered total cell number in both populations, indicating active depletion of spermatocytes and spermatids
• sperm counts are lower in 12 week old males
• about 2-fold increase in apoptotic cells, mainly spermatocytes and early round spermatids, in the testes
• seminiferous tubules at around stage VI exhibit round spermatids with an increase in unrepaired DNA double-strand breaks
• total motility and progressive motility of sperm are lower in 12 week old males
• seminiferous epithelium is thinner in 3 month old testes and contains numerous vacuoles
• 12 week old males exhibit reduced testis size
• 12 week old males exhibit reduced testis weight
• caput and cauda epididymides are smaller
• males exhibit reduced fertility in 5-month long fertility tests, with some males completely infertile and others close to normal fertility

endocrine/exocrine glands
• seminiferous epithelium is thinner in 3 month old testes and contains numerous vacuoles
• 12 week old males exhibit reduced testis size
• 12 week old males exhibit reduced testis weight

cellular
• epididymal sperm shows a variety of morphological abnormalities in the flagellum including coiled or headless flagellum
• epididymal sperm shows a variety of morphological abnormalities in the sperm head like crooked, round, or bent heads
• seminiferous epithelium is thinner in 3 month old testes and contains numerous vacuoles, indicative of germ cell depletion
• presence of numerous degenerating/degenerated spermatids, or even spermatocytes in the epididymis; this enhanced depletion of spermatocytes and spermatids and the production of deformed spermatozoa are seen only in mice older than 2 months and no differences are seen during the first wave of spermatogenesis
• proportional increase of meiotic cells (i.e.spermatocytes) and decrease in haploid cells (spermatids) despite lowered total cell number in both populations, indicating active depletion of spermatocytes and spermatids
• sperm counts are lower in 12 week old males
• about 2-fold increase in apoptotic cells, mainly spermatocytes and early round spermatids, in the testes
• seminiferous tubules at around stage VI exhibit round spermatids with an increase in unrepaired DNA double-strand breaks
• total motility and progressive motility of sperm are lower in 12 week old males




Genotype
MGI:6156877
cn15
Allelic
Composition
Tdrd5tm1c(EUCOMM)Hmgu/Tdrd5tm1c(EUCOMM)Hmgu
Tg(Stra8-icre)1Reb/0
Genetic
Background
involves: C57BL/6N * FVB/NJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tdrd5tm1c(EUCOMM)Hmgu mutation (0 available); any Tdrd5 mutation (30 available)
Tg(Stra8-icre)1Reb mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• average 50% reduction
• average 50% of wild-type weight

reproductive system
• average 50% reduction
• average 50% of wild-type weight
• at round spermatid stage; no elongating spermatids or spermatozoa formed




Genotype
MGI:6369095
cn16
Allelic
Composition
Crisp2tm1c(KOMP)Mbp/Crisp2tm1c(KOMP)Mbp
Tg(Stra8-icre)1Reb/0
Genetic
Background
involves: C57BL/6N * FVB/NJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Crisp2tm1c(KOMP)Mbp mutation (0 available); any Crisp2 mutation (4 available)
Tg(Stra8-icre)1Reb mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
N
• female fertility is similar to controls
• significant reduction in the percentage of sperm displaying rapid forms of progressive motility
• show a 28.7% reduction in the number of sperm showing any form of motility
• decrease in the percentage of sperm that undergo progesterone-evoked acrosome reaction
• males are sub-fertile
• however, testes weight and sperm production and histology are similar to controls

cellular
• significant reduction in the percentage of sperm displaying rapid forms of progressive motility
• show a 28.7% reduction in the number of sperm showing any form of motility
• decrease in the percentage of sperm that undergo progesterone-evoked acrosome reaction




Genotype
MGI:6274241
cn17
Allelic
Composition
Katnal2tm1c(EUCOMM)Wtsi/Katnal2tm1c(EUCOMM)Wtsi
Tg(Stra8-icre)1Reb/0
Genetic
Background
involves: C57BL/6N * FVB/NJ
Cell Lines EPD0656_4_A03
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Katnal2tm1c(EUCOMM)Wtsi mutation (0 available); any Katnal2 mutation (198 available)
Tg(Stra8-icre)1Reb mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype

Spermatogenic defects in Katnal2tm1a(EUCOMM)Wtsi/Katnal2tm1a(EUCOMM)Wtsi Tg(Stra8-icre)1Reb/0 mice

reproductive system
• males exhibit a sperm tail phenotype identical to that seen in Katnal2m1Anu homozygotes
• elongating spermatids frequently exhibit abnormal nuclear morphology
• males exhibit a manchette phenotype identical to that seen in Katnal2m1Anu homozygotes
• multinucleated symplasts are frequently observed in the seminiferous epithelium
• multinucleated symplasts are frequently observed in the seminiferous epithelium
• total epididymal sperm content is reduced by 94.1% relative to control mice; however, testis weight and daily sperm output in the testes are normal, indicating a massive failure of spermiation
• retained elongated spermatids are often observed in stage IX tubules, unlike in control mice
• however, in contrast to Katnal2m1Anu homozygotes, tubulobulbar complex and residual body formation is similar to that observed in control mice

cellular
• males exhibit a sperm tail phenotype identical to that seen in Katnal2m1Anu homozygotes
• elongating spermatids frequently exhibit abnormal nuclear morphology
• males exhibit a manchette phenotype identical to that seen in Katnal2m1Anu homozygotes
• multinucleated symplasts are frequently observed in the seminiferous epithelium

endocrine/exocrine glands
• multinucleated symplasts are frequently observed in the seminiferous epithelium




Genotype
MGI:5763275
cn18
Allelic
Composition
Tg(RNU6-RNAi:Rhox3)2Wilk/0
Tg(Stra8-icre)1Reb/0
Genetic
Background
involves: FVB/NJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(RNU6-RNAi:Rhox3)2Wilk mutation (0 available)
Tg(Stra8-icre)1Reb mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
• abnormal of elongated spermatids
• few sperm in the cauda epididymides (greater than 100 fold reduction)
• of pachytene spermatocyte in seminiferous epithelial cycle stage IV
• of spermatocytes undergoing meiotic metaphase in stage XII
• of abnormal elongated spermatids
• decreased diameter
• however, seminal vesicle weight is normal
• defective meiotic prophase

cellular
• abnormal of elongated spermatids
• few sperm in the cauda epididymides (greater than 100 fold reduction)
• of pachytene spermatocyte in seminiferous epithelial cycle stage IV
• of spermatocytes undergoing meiotic metaphase in stage XII
• of abnormal elongated spermatids

endocrine/exocrine glands
• decreased diameter
• however, seminal vesicle weight is normal




Genotype
MGI:5904161
cn19
Allelic
Composition
Tg(Stra8-icre)1Reb/0
Zscan21tm1.1Jiwa/Zscan21tm1.1Jiwa
Genetic
Background
involves: FVB/NJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Stra8-icre)1Reb mutation (2 available)
Zscan21tm1.1Jiwa mutation (0 available); any Zscan21 mutation (29 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
• testes show no round spermatids
• testes show few spermatocytes, without round spermatids
• testis weight of adult males is less than 40% of heterozygotes
• unsynapsed or partially synapsed chromosomes are seen in spermatocytes indicating failure of chromosomal synapsis
• spermatocytes do not progress to meiotic prophase I
• males are infertile, with 6-8 week old males failing to produce offspring after natural mating with wild-type females for more than 4 months

cellular
• testes show no round spermatids
• testes show few spermatocytes, without round spermatids
• failure of double-strand DNA break repair in spermatocytes as indicated by reduced MLH1 foci and RAD51 foci

endocrine/exocrine glands
• testis weight of adult males is less than 40% of heterozygotes

homeostasis/metabolism
• failure of double-strand DNA break repair in spermatocytes as indicated by reduced MLH1 foci and RAD51 foci




Genotype
MGI:5763277
cn20
Allelic
Composition
Rhoxtm1Wilk/Rhoxtm1Wilk
Tg(RNU6-RNAi:Rhox3)2Wilk/0
Tg(Stra8-icre)1Reb/0
Genetic
Background
involves: FVB/NJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rhoxtm1Wilk mutation (0 available); any Rhox mutation (0 available)
Tg(RNU6-RNAi:Rhox3)2Wilk mutation (0 available)
Tg(Stra8-icre)1Reb mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• dramatic reduction of post-meiotic germ cells
• dramatic

reproductive system
• dramatic reduction of post-meiotic germ cells
• dramatic
• 80% reduction

endocrine/exocrine glands
• 80% reduction




Genotype
MGI:3850155
tg21
Allelic
Composition
Tg(Stra8-icre)1Reb/0
Genetic
Background
involves: FVB
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Stra8-icre)1Reb mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
normal phenotype
• mice are viable and fertile; no phenotypic details are provided





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last database update
08/04/2020
MGI 6.15
The Jackson Laboratory