Analysis Tools
normal phenotype
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• homozygous mice developed normally with unperturbed Cops5 expression
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Allele Symbol Allele Name Allele ID |
Cops5tm1Rpar targeted mutation 1, Ruggero Pardi MGI:3775801 |
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| Summary |
5 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• homozygous mice developed normally with unperturbed Cops5 expression
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• an 80-90% reduction compared to control showing no rescue of the reduced thymic cellularity resulting from Cops5 deficiency
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• an 80-90% reduction compared to control showing no rescue of the reduced thymic cellularity resulting from Cops5 deficiency
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• an 80-90% reduction compared to control showing no rescue of the reduced thymic cellularity resulting from Cops5 deficiency
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• TUNEL staining revealed a dramatic increase in the presence of apoptotic germ cells in the seminiferous tubules
• Western blot analysis showed that PARP1 and FANK1 expression levels are dramatically reduced, whereas Caspase-3 expression level is significantly increased
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• less than 10% of sperm are motile; however, their curvilinear velocity is significantly reduced
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• most sperm display no mobility
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• seminiferous tubule structure is totally disrupted
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• large vacuoles are present in the epithelium and these are likely the spaces remaining after Sertoli cell phagocytosis of apoptotic cells
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• adult testis size is markedly reduced
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• testis weight and testis weight/body weight are reduced to about one third of controls
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• spermatogenesis is severely impaired: a number of degenerating germ cells are observed in the lumen of the seminiferous epithelium, with very few developed elongated spermatids
• round bodies (possibly sloughed germ cells) and cellular debris are seen in the cauda epididymis lumen
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• cauda epididymal sperm counts are severely reduced
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• >80% of sperm exhibit abnormal morphology
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• few surviving spermatids exhibit little to no staining with acrosome marker peanut-lectin (PNA) while expression level of SPACA1 (another acrosomal component) is significantly reduced
• testicular IFT20 expression level is significantly reduced; although IFT20 is still found in the Golgi apparatus of spermatocytes, it is no longer localized in the acrosome of round spermatids and, instead, it is present as individual vesicles in the cytoplasm
• acrosome formation is absent in most round spermatids; in other spermatids, an abnormal acrosome is formed, or a very thin acrosome is attached to the Sertoli cell membrane by an abnormal ectoplasmic specialization
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• few germ cells that develop into elongating spermatids exhibit abnormal nuclear structures
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• elongating spermatids are rarely observed
• few germ cells that develop into elongating spermatids exhibit abnormal nuclei
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• when bred with adult wild-type females of known fertility, none of the 6-wk-old or adult males tested sired any pups/litters during a 2-month breeding period, indicating complete male sterility
• however, mating behavior and vaginal plug formation are normal
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• cauda epididymal sperm counts are severely reduced
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• >80% of sperm exhibit abnormal morphology
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• few surviving spermatids exhibit little to no staining with acrosome marker peanut-lectin (PNA) while expression level of SPACA1 (another acrosomal component) is significantly reduced
• testicular IFT20 expression level is significantly reduced; although IFT20 is still found in the Golgi apparatus of spermatocytes, it is no longer localized in the acrosome of round spermatids and, instead, it is present as individual vesicles in the cytoplasm
• acrosome formation is absent in most round spermatids; in other spermatids, an abnormal acrosome is formed, or a very thin acrosome is attached to the Sertoli cell membrane by an abnormal ectoplasmic specialization
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• few germ cells that develop into elongating spermatids exhibit abnormal nuclear structures
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• elongating spermatids are rarely observed
• few germ cells that develop into elongating spermatids exhibit abnormal nuclei
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• TUNEL staining revealed a dramatic increase in the presence of apoptotic germ cells in the seminiferous tubules
• Western blot analysis showed that PARP1 and FANK1 expression levels are dramatically reduced, whereas Caspase-3 expression level is significantly increased
|
|
|
• less than 10% of sperm are motile; however, their curvilinear velocity is significantly reduced
|
|
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• most sperm display no mobility
|
|
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• seminiferous tubule structure is totally disrupted
|
|
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• large vacuoles are present in the epithelium and these are likely the spaces remaining after Sertoli cell phagocytosis of apoptotic cells
|
|
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• adult testis size is markedly reduced
|
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• testis weight and testis weight/body weight are reduced to about one third of controls
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• in DN and DP thymocytes
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• a severely hypoplastic medulla
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• an 80-90% reduction
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• a marked decrease in the proportion of DP and SP thymocytes
• DN thymocytes percentage and absolute numbers were not significantly altered
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• a marked decrease in the proportion of DP and SP thymocytes
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• in DN and DP thymocytes
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• a severely hypoplastic medulla
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• an 80-90% reduction
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• a marked decrease in the proportion of DP and SP thymocytes
• DN thymocytes percentage and absolute numbers were not significantly altered
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• a marked decrease in the proportion of DP and SP thymocytes
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• cell cycle arrest at S/G2/M phase in DN thymocyte
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• in DN and DP thymocytes
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• a severely hypoplastic medulla
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• an 80-90% reduction
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• an 80-90% reduction compared to control
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• an 80-90% reduction compared to control
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• an 80-90% reduction compared to control
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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