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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Tg(Nkx2-1-cre)2Sand
transgene insertion 2, Stewart Anderson
MGI:3773076
Summary 11 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Ptentm1Hwu/Pten+
Tg(Nkx2-1-cre)2Sand/0
B6.Cg-Ptentm1Hwu Tg(Nkx2-1-cre)2Sand MGI:5517709
cn2
Ptentm1Hwu/Ptentm1Hwu
Tg(Nkx2-1-cre)2Sand/0
B6.Cg-Ptentm1Hwu Tg(Nkx2-1-cre)2Sand MGI:5517706
cn3
Stk3tm1.1Jav/Stk3tm1.1Jav
Stk4tm1Kina/Stk4tm1Kina
Tg(Nkx2-1-cre)2Sand/?
involves: 129 * C57BL/6 MGI:5502638
cn4
Dbx1tm2Jcor/Dbx1tm1Tmj
Tg(Nkx2-1-cre)2Sand/0
involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 MGI:5697928
cn5
Ptentm1Hwu/Pten+
Tg(Nkx2-1-cre)2Sand/0
involves: 129S4/SvJae * BALB/c * C57BL/6 MGI:5517675
cn6
Ptentm1Hwu/Ptentm1Hwu
Tg(Nkx2-1-cre)2Sand/0
involves: 129S4/SvJae * BALB/c * C57BL/6 MGI:5014514
cn7
Ptentm1Hwu/Ptentm1Hwu
Tg(Nkx2-1-cre)2Sand/0
involves: 129S4/SvJae * C57BL/6 MGI:4836606
cn8
Yy1tm2.1Yshi/Yy1tm2.1Yshi
Tg(Nkx2-1-cre)2Sand/0
involves: 129S4/SvJae * C57BL/6J MGI:5902325
cn9
Atp1b1tm1.1Zboro/Atp1b1tm1.1Zboro
Tg(Nkx2-1-cre)2Sand/0
involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6 MGI:5788016
cn10
Igf1rtm1Jcbr/Igf1rtm1Jcbr
Tg(Nkx2-1-cre)2Sand/0
involves: C57BL/6 MGI:5904181
cn11
Kmt2dtm1.1Kaig/Kmt2dtm1.1Kaig
Tg(Nkx2-1-cre)2Sand/0
involves: C57BL/6 MGI:6515626


Genotype
MGI:5517709
cn1
Allelic
Composition
Ptentm1Hwu/Pten+
Tg(Nkx2-1-cre)2Sand/0
Genetic
Background
B6.Cg-Ptentm1Hwu Tg(Nkx2-1-cre)2Sand
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ptentm1Hwu mutation (16 available); any Pten mutation (81 available)
Tg(Nkx2-1-cre)2Sand mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• altered thyroid structure with normal areas that include colloid-filled follicles and normal areas with focal hyperplasia, polynuclear cells, small nonencapsulated areas of hypercellularity with solid and/or mircofollicular patterns and internal hemorrhage
• 100% of mutants develop differentiated follicular tumors of the thyroid after about 2 years of age

homeostasis/metabolism
• P14 mutants show a mild decrease of T4 but normal TSH

neoplasm
• 100% of mutants develop differentiated follicular tumors of the thyroid after about 2 years of age

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
thyroid gland follicular carcinoma DOID:3962 OMIM:188470
J:197590




Genotype
MGI:5517706
cn2
Allelic
Composition
Ptentm1Hwu/Ptentm1Hwu
Tg(Nkx2-1-cre)2Sand/0
Genetic
Background
B6.Cg-Ptentm1Hwu Tg(Nkx2-1-cre)2Sand
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ptentm1Hwu mutation (16 available); any Pten mutation (81 available)
Tg(Nkx2-1-cre)2Sand mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• at P14, thyroid architecture is altered, with multilayered epithelial cells surrounding the colloid lumen and with some dysmorphic follicles containing abnormal material inside
• dysmorphic follicles containing abnormal material inside and multiple degenerative follicles which have varying degrees of colloid depletion are seen at P14
• increase in thyroid size is already seen at E15.5 and is striking by P14
• increase in epithelial cell proliferation rate at E15.5 and P14
• Background Sensitivity: at P14, epithelial cell proliferation rates are higher on the congenic C57BL/6 background than on the BALB/c background
• at P14, thyroid structure is completely altered with multiple degenerative follicles, which have varying degrees of colloid depletion, resembling a follicular adenoma

homeostasis/metabolism
• decrease in T4 hormonal levels at P14

mortality/aging
• all mutants die before 2 weeks of age due to compression of the trachea and esophagus by the enlarged thyroid

neoplasm
• at P14, thyroid structure is completely altered with multiple degenerative follicles, which have varying degrees of colloid depletion, resembling a follicular adenoma




Genotype
MGI:5502638
cn3
Allelic
Composition
Stk3tm1.1Jav/Stk3tm1.1Jav
Stk4tm1Kina/Stk4tm1Kina
Tg(Nkx2-1-cre)2Sand/?
Genetic
Background
involves: 129 * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Stk3tm1.1Jav mutation (0 available); any Stk3 mutation (47 available)
Stk4tm1Kina mutation (0 available); any Stk4 mutation (72 available)
Tg(Nkx2-1-cre)2Sand mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• a markedly sick appearance at birth and almost all mice died within the first 15 d after birt
• born at normal Mendelian ratio
• lung size and dry lung weight were comparable between Mst1/2 dKO mice and control mice at E15.5 and E18.5

respiratory system
• at E18.5, lungs showed a markedly increased number of proliferative cells and mildly diminished cell death
• no abnormal proliferation of mesenchymal cells
• displayed spontaneous infiltration of inflammatory cells
• No bacteria and fungi were detected in lung sections or in cytospins of bronchoalveolar lavage fluid, ruling out the possibility of infection
• Lung Epithelial Cells Causes Abnormal Lung Maturation: Impaired Surfactant Homeostasis and Delayed Differentiation of Type I and II Pneumocytes
• vascularization was normal
• reduced aerated space compacted with immature-looking cuboidal cells
• lung showed severe accumulation of inflammatory cells in the alveolar space and bronchiole with multiple atelectasis and emphysematous lesions
• lung showed severe accumulation of inflammatory cells in the alveolar space and bronchiole with multiple atelectasis and emphysematous lesions
• at around post natal day 5

immune system
• displayed spontaneous infiltration of inflammatory cells
• No bacteria and fungi were detected in lung sections or in cytospins of bronchoalveolar lavage fluid, ruling out the possibility of infection

cellular
• at E18.5, lungs showed a markedly increased number of proliferative cells and mildly diminished cell death
• no abnormal proliferation of mesenchymal cells




Genotype
MGI:5697928
cn4
Allelic
Composition
Dbx1tm2Jcor/Dbx1tm1Tmj
Tg(Nkx2-1-cre)2Sand/0
Genetic
Background
involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dbx1tm1Tmj mutation (1 available); any Dbx1 mutation (25 available)
Dbx1tm2Jcor mutation (1 available); any Dbx1 mutation (25 available)
Tg(Nkx2-1-cre)2Sand mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• attenuated response to predator (rat) odor is observed in females ( as measured by corticosterone levels in the lateral hypothalamus area) as compared to control females
• males (either control or mutant) do not exhibit a response
• slices from the lateral hypothalamic area (LH) exhibit 85% lower spike density in low glucose
• slices from the lateral hypothalamic area (LH) exhibit 50% lower average spike frequency in high glucose
• however, glucose tolerance is not affected

behavior/neurological
• females exhibit a similar response following presentation with either rat or benign odor in contrast to controls
• females exhibit fewer and shorter escape behaviors as compared to controls following presentation with rat odor
• females eat less than controls on a restricted diet
• decrease in amount of food consumed following a 12 hour fast is observed in females, but not males
• however, daily food consumption is similar to controls
• activity, as determined by horizontal line crossings, is increased in males, but not in females

growth/size/body
• decreased body weight in males, but not females, is observed after weaning
• females exhibit a decrease in body weight under restricted or high fat diets

adipose tissue
• males exhibit decrease in body fat composition after 7 weeks on high fat diet




Genotype
MGI:5517675
cn5
Allelic
Composition
Ptentm1Hwu/Pten+
Tg(Nkx2-1-cre)2Sand/0
Genetic
Background
involves: 129S4/SvJae * BALB/c * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ptentm1Hwu mutation (16 available); any Pten mutation (81 available)
Tg(Nkx2-1-cre)2Sand mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• mutants have an enlarged thyroid at 2 years of age and exhibit a goiter-like phenotype, but do not show development of tumors

homeostasis/metabolism
• P14 mutants show an increase in T4 levels




Genotype
MGI:5014514
cn6
Allelic
Composition
Ptentm1Hwu/Ptentm1Hwu
Tg(Nkx2-1-cre)2Sand/0
Genetic
Background
involves: 129S4/SvJae * BALB/c * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ptentm1Hwu mutation (16 available); any Pten mutation (81 available)
Tg(Nkx2-1-cre)2Sand mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
homeostasis/metabolism
N
• Background Sensitivity: unlike on the congenic C57BL/6 background, mutants on the BALB/c background have normal T4 and TSH levels
• after naphthalene administration to induce lung injury, the proximal airway epithelium at 3 and 7 days post injury appears intact with no signs of injury
• after naphthalene administration to induce lung injury, the level of injury in the bronchial airway epithelium is reduced and repair is enhanced compared to controls

respiratory system
• mutants exhibit an increase in the proliferation rate of the epithelial cells in E15.5 lungs
• expansion of epithelial cell populations occurs in multiple progenitor cell niches of the lungs including the tracheal basal cells in the proximal lung, the neuroepithelial bodies (NEB) in the distal bronchi and the progenitor cells occupying the bronchioalveolarduct junction (BADJ) region
• lungs exhibit increased cell proliferation and decreased apoptosis
• lungs at 8 weeks of age sometimes contain a mass consisting of epithelial cells (putative progenitor cell masses) around the BADJ area; masses are slow growing and are organized into ductlike structures
• progressive epithelial hyperplasia extending from the trachea to the small bronchioles is seen in the proximal lung epithelium from early stages of lung development and in adults
• marker analysis indicates a block in transition from precursor to terminally differentiated cell types indicating impaired epithelial cell fate determination in the lung
• increase in number of Clara cells in the lungs and a decrease in the number of ciliated cells, the terminally differentiated progeny of Clara cells

cellular
• mutants exhibit an increase in the proliferation rate of the epithelial cells in E15.5 lungs
• expansion of epithelial cell populations occurs in multiple progenitor cell niches of the lungs including the tracheal basal cells in the proximal lung, the neuroepithelial bodies (NEB) in the distal bronchi and the progenitor cells occupying the bronchioalveolarduct junction (BADJ) region

endocrine/exocrine glands
• Background Sensitivity: at P14, epithelial cell proliferation rates in the thyroid are lower on the mixed BALB/c background than on the congenic C57BL/6 background
• at P14, thyroid architecture is conserved but an increase in the number of epithelial cells along the follicles is seen, resembling a goiter disease

mortality/aging
N
• Background Sensitivity: mutants survive to adulthood on the BALB/c background without presenting any obvious pathological conditions unlike mice on the congenic C57BL/6 background

growth/size/body
• progressive epithelial hyperplasia extending from the trachea to the small bronchioles is seen in the proximal lung epithelium from early stages of lung development and in adults




Genotype
MGI:4836606
cn7
Allelic
Composition
Ptentm1Hwu/Ptentm1Hwu
Tg(Nkx2-1-cre)2Sand/0
Genetic
Background
involves: 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ptentm1Hwu mutation (16 available); any Pten mutation (81 available)
Tg(Nkx2-1-cre)2Sand mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
respiratory system
• increase in the numbers of Clara cells in the lungs




Genotype
MGI:5902325
cn8
Allelic
Composition
Yy1tm2.1Yshi/Yy1tm2.1Yshi
Tg(Nkx2-1-cre)2Sand/0
Genetic
Background
involves: 129S4/SvJae * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Nkx2-1-cre)2Sand mutation (2 available)
Yy1tm2.1Yshi mutation (0 available); any Yy1 mutation (34 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• a small percentage of mutants survive up to weaning age
• most mutants die at birth but some survive up to weaning age

neoplasm
• mice surviving to weaning exhibit characteristics of an evolving type I pleuropulmonary blastoma-like phenotype

respiratory system
• embryos exhibit cysts in the proximal region of lung lobes
• Type I and II pneumocytes, but not club and ciliated cells, are present along the cystic epithelium
• P21 lungs show multiocular cysts and variable septal thickness, small mesenchymal cells within the cyst walls, high proliferation levels of mesenchymal cells within the cystic wall
• increase in proliferation in E18.5 lungs

growth/size/body
• embryos exhibit cysts in the proximal region of lung lobes
• Type I and II pneumocytes, but not club and ciliated cells, are present along the cystic epithelium
• P21 lungs show multiocular cysts and variable septal thickness, small mesenchymal cells within the cyst walls, high proliferation levels of mesenchymal cells within the cystic wall

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
pleuropulmonary blastoma DOID:4769 J:239777




Genotype
MGI:5788016
cn9
Allelic
Composition
Atp1b1tm1.1Zboro/Atp1b1tm1.1Zboro
Tg(Nkx2-1-cre)2Sand/0
Genetic
Background
involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Atp1b1tm1.1Zboro mutation (1 available); any Atp1b1 mutation (20 available)
Tg(Nkx2-1-cre)2Sand mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• pups die at 2-3 weeks of age




Genotype
MGI:5904181
cn10
Allelic
Composition
Igf1rtm1Jcbr/Igf1rtm1Jcbr
Tg(Nkx2-1-cre)2Sand/0
Genetic
Background
involves: C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igf1rtm1Jcbr mutation (0 available); any Igf1r mutation (85 available)
Tg(Nkx2-1-cre)2Sand mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
growth/size/body
• mice are significantly overweight after puberty

homeostasis/metabolism
• mice show a delay in recovery of terminal bronchiolar epithelium after naphthalene treatment to induce club cell damage, with a delay in the differentiation/regeneration of club and ciliated cells
• mice show increased proliferation of bronchiolar epithelial cells during repair of the epithelium after naphthalene treatment to induce damage and club cell ablation

respiratory system
• smaller epithelial cell size in terminal bronchioles
• lower proportion of Scgb1a1+ club cells
• distal bronchiolar epithelium shows morphological changes including lower cell density, flatter cells with elongated nuclei and frequent interruptions of normal columnar organization
• however, no gross morphological alterations are seen in AEC2 cells or in the surrounding alveolar parenchyma
• proliferation of airway club cells is enhanced in terminal bronchial epithelium




Genotype
MGI:6515626
cn11
Allelic
Composition
Kmt2dtm1.1Kaig/Kmt2dtm1.1Kaig
Tg(Nkx2-1-cre)2Sand/0
Genetic
Background
involves: C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Kmt2dtm1.1Kaig mutation (1 available); any Kmt2d mutation (167 available)
Tg(Nkx2-1-cre)2Sand mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• marker analysis suggests that the development of GHRH-neurons, rather than just the expression of Ghrh, is impaired in mutant mice
• however, the number of AgRP-neurons is unchanged and neither Sf1+ or Trh+ cell numbers are altered
• growth hormone-releasing hormone (GHRH)-producing neurons expressing Ghrh and Th are reduced at E14.5-E15.5, indicating that development of GHRH-neurons is impaired
• mice show a reduction of GHRH-neurons at P65

homeostasis/metabolism

cellular
• marker analysis suggests that the development of GHRH-neurons, rather than just the expression of Ghrh, is impaired in mutant mice
• however, the number of AgRP-neurons is unchanged and neither Sf1+ or Trh+ cell numbers are altered





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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory