Mouse Genome Informatics
cn1
    Meox2tm1(cre)Sor/Meox2+
Slc40a1tm2Nca/Slc40a1tm2Nca

involves: 129S4/SvJae * 129S6/SvEvTac
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
N
• unlike homozygous null mice, mice with conditional deletion in embryonic tissues that retain expression in the extraembryonic visceral endoderm and in the placenta survive past birth (J:129846)

hematopoietic system
• at 18 - 22 days of age
• hypochromic at P10
• reduced in both mature erythrocytes and reticulocytes at 18 - 22 days of age
• at 18 - 22 days of age
• at P10
• severe iron deficient anemia
• at P10
• at P10 and at P18 - P22
• significant iron accumulation in splenic macrophages at P12
• at P18 - P22 spleen iron are increased 4.4-fold increase compared to controls

growth/size
• apparent within a few days of birth
• difference from controls becomes more prominent with age

homeostasis/metabolism
• significant iron accumulation in duodenal enterocytes at P12
• significant iron accumulation in splenic macrophages at P12
• at P18 - P22 spleen iron are increased 4.4-fold increase compared to controls
• significant iron accumulation in the Kupffer cells and hepatocytes at P12
• at P18 - P22 iron levels in the liver are reduced by 1.7-fold compared to controls

digestive/alimentary system
• significant iron accumulation in duodenal enterocytes at P12

immune system
• significant iron accumulation in splenic macrophages at P12
• at P18 - P22 spleen iron are increased 4.4-fold increase compared to controls

liver/biliary system
• significant iron accumulation in the Kupffer cells and hepatocytes at P12
• at P18 - P22 iron levels in the liver are reduced by 1.7-fold compared to controls

integument
• apparent within a few days of birth
• difference from controls becomes more prominent with age


Mouse Genome Informatics
cn2
    Slc40a1tm2Nca/Slc40a1tm2Nca
Tg(Vil-cre)20Syr/0

involves: 129S4/SvJae * 129S6/SvEvTac * C57BL/6 * DBA/2
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• no mice are found at birth due to recombination in the extraembryonic visceral endoderm


Mouse Genome Informatics
cn3
    Slc40a1tm2Nca/Slc40a1tm2Nca
Tg(Vil-cre/ERT2)23Syr/0

involves: 129S4/SvJae * 129S6/SvEvTac * C57BL/6 * DBA/2
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
hematopoietic system
• by 6 - 7 weeks after the start of tamoxifen treatment, average hematocrit is 11.2 +/- 1.6% compared to 45.3 +/- 5.9% in controls without the cre transgene
• parenteral treatment with iron dextran restores hematocrits in tamoxifen treated mice to levels similar to control mice that do not carry the cre transgene
• by 6 - 7 weeks after the start of tamoxifen treatment, average hemoglobin concentration is 2.8 +/- 0.6 g/dl compared to 13.8 +/- 1.8 g/dl in controls without the cre transgene
• visibly anemic by 8 days after the first tamoxifen injection
• severe iron deficient anemia develops by 6 - 7 weeks after the start of tamoxifen treatment
• a 4.7-fold decrease in splenic nonheme iron is seen after tamoxifen treatment

homeostasis/metabolism
• significant iron accumulation in duodenal enterocytes after tamoxifen treatment
• a 4.7-fold decrease in splenic nonheme iron is seen after tamoxifen treatment
• a 5.5-fold decrease in hepatic nonheme iron is seen after tamoxifen treatment

digestive/alimentary system
• significant iron accumulation in duodenal enterocytes after tamoxifen treatment

immune system
• a 4.7-fold decrease in splenic nonheme iron is seen after tamoxifen treatment

liver/biliary system
• a 5.5-fold decrease in hepatic nonheme iron is seen after tamoxifen treatment