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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
St8sia4tm1.1Mifu
targeted mutation 1.1, Minoru Fukuda
MGI:3762640
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cx1
St8sia2tm1Jxm/St8sia2tm1Jxm
St8sia4tm1.1Mifu/St8sia4tm1.1Mifu
involves: C57BL/6 MGI:3762774
cx2
St8sia2tm1Jxm/St8sia2tm1Jxm
St8sia4tm1.1Mifu/St8sia4tm1.1Mifu
Tg(Thy1-YFP)HJrs/?
involves: C57BL/6 * CBA MGI:3762775


Genotype
MGI:3762774
cx1
Allelic
Composition
St8sia2tm1Jxm/St8sia2tm1Jxm
St8sia4tm1.1Mifu/St8sia4tm1.1Mifu
Genetic
Background
involves: C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
St8sia2tm1Jxm mutation (1 available); any St8sia2 mutation (23 available)
St8sia4tm1.1Mifu mutation (0 available); any St8sia4 mutation (26 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• most mice die between P0 and P10 with some mice surviving until P20

nervous system
• neurons and their precursors in the cortex and subventricular zone undergo more apoptosis than in wild-type mice
• however, hippocampal cells do not undergo apoptosis except for those located in the lateral ventricle
• in culture, neurospheres differentiate into GFAP+ cells that have astrocyte-like morphology unlike wild-type neurospehre cells
• in the presence of PDGF more neurospheres differentiated into GFAP+ cells (47.0+/-1.5% compared to 31.6+/-4.8% of wild-type neurospheres) while in the presence of BDNF fewer do so (21.6+/-5.8% compared to 25.6+/-5.2 of wild-type neurospheres)
• neuron migration is impaired and in culture neurons move 8 to 15% slower than wild-type neurons when plated on laminin
• however, proliferation and differentiation of neurons is normal
• at P0 the rostral migratory stream is thicker than in wild-type mice but by P10 it is thinner than in wild-type mice
• GABAnergic interneurons in the cerebral cortex are decreased to 20% of the number found in wild-type mice
• hippocampal cells located in the lateral ventricle undergo apoptosis
• coronal and sagittal brain sections reveal an increase in the size of the lateral ventricle as well as a thinner cortex
• lateral ventricles increase with age
• the corpus callosum is thinner than in wild-type mice or partially absent
• the striatum is thinner than in wild-type mice
• the diencephalons is thinner than in wild-type mice
• hippocampal cells located in the lateral ventricle undergo apoptosis
• mice have fewer cortical cells than in wild-type mice
• neurons and their precursors in the cortex and subventricular zone undergo more apoptosis than in wild-type mice
• GABAnergic interneurons in the cerebral cortex are decreased to 20% of the number found in wild-type mice
• in layers II/III and IV of the neocortex
• unlike in wild-type mice, pyramidal cells are detected in the subventricular zone
• coronal and sagittal brain sections reveal an increase in the size of the lateral ventricle as well as a thinner cortex
• the cerebral cortex becomes thinner with age
• at P10, the olfactory bulb is smaller than in wild-type mice due to impaired movement of neuroblasts from the sub-ventricular zone to the olfactory bulb
• neurons and their precursors in the cortex and subventricular zone undergo more apoptosis than in wild-type mice
• unlike in wild-type mice, pyramidal cells are detected in the subventricular zone
• glial cell migration is impaired whereby GFAP+ cells are confined to the subventricular zone close to the lateral ventricle
• unlike in wild-type mice, GFAP+ cells exhibit an elongated morphology
• the number of GFAP+ astrocytes in the hippocampus is decreased to 30% of wild-type hippocampus

behavior/neurological
• mice exhibit slow, weak and uncoordinated movements

growth/size/body
• mice are smaller than normal

cellular
• neurons and their precursors in the cortex and subventricular zone undergo more apoptosis than in wild-type mice
• however, hippocampal cells do not undergo apoptosis except for those located in the lateral ventricle
• in culture, neurospheres differentiate into GFAP+ cells that have astrocyte-like morphology unlike wild-type neurospehre cells
• in the presence of PDGF more neurospheres differentiated into GFAP+ cells (47.0+/-1.5% compared to 31.6+/-4.8% of wild-type neurospheres) while in the presence of BDNF fewer do so (21.6+/-5.8% compared to 25.6+/-5.2 of wild-type neurospheres)
• neuron migration is impaired and in culture neurons move 8 to 15% slower than wild-type neurons when plated on laminin
• however, proliferation and differentiation of neurons is normal




Genotype
MGI:3762775
cx2
Allelic
Composition
St8sia2tm1Jxm/St8sia2tm1Jxm
St8sia4tm1.1Mifu/St8sia4tm1.1Mifu
Tg(Thy1-YFP)HJrs/?
Genetic
Background
involves: C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
St8sia2tm1Jxm mutation (1 available); any St8sia2 mutation (23 available)
St8sia4tm1.1Mifu mutation (0 available); any St8sia4 mutation (26 available)
Tg(Thy1-YFP)HJrs mutation (3 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• unlike in wild-type mice, the projections of dendrites and axons from pyramidal cells are disorganized
• the number of pyramidal cells is decreased to 25% of that in wild-type mice





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last database update
03/12/2024
MGI 6.23
The Jackson Laboratory