Phenotypes associated with this allele

• Allele Symbol: Tg(H2-K-Myc)1Brn
• Allele Name: transgene insertion 1, Anton Berns
• Allele ID: MGI:3761465
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Lrfn2^Tg(CD2-Myb/en)5Kmw/Lrfn2^+ Tg(H2-K-Myc)1Brn/?	involves: C57BL/10 * CBA * FVB/N	MGI:3761491

Genotype
MGI:3761491

cx1

• Allelic Composition: Lrfn2^{Tg(CD2-Myb/en)5Kmw}/Lrfn2⁺; Tg(H2-K-Myc)1Brn/?
• Genetic Background: involves: C57BL/10 * CBA * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

premature death ( J:125805 )

• all mice had to be sacrificed at 14 weeks because of their enlarged peripheral lymph nodes

immune system

lymph node hemorrhage ( J:125805 )

• mice exhibit diffuse hemorrhaging with necrosis in the lymph nodes

enlarged spleen ( J:125805 )

spleen hyperplasia ( J:125805 )

• total cell numbers in inguinal and auxiliary lymph nodes is increased between 5x10⁸ and 25x10⁸ with the greatest numbers observed in the older mice

abnormal lymph node morphology ( J:125805 )

• lymph nodes contain large hematomas with cystic degeneration and diffuse hemorrhage with necrosis and become a site of extramedullary hematopoiesis

• lymph nodes contain many nonlymphoid cells that are a mixture of late-stage erythroblasts, reticulocytes and mature erythrocytes

• the majority of abnormal cells in the lymph node are orthochromatophilic eryththroblasts with a small amount of R2 and R4 gate cells indicating that all stages of erythroblasts are present

• fewer than 2% of lymph node cells express T cell, B cell or granulocyte markers

lymph node necrosis ( J:125805 )

enlarged lymph nodes ( J:125805 )

• between 4 and 10 weeks all mice exhibit enlarged lymph nodes (up to 1 cm in diameter at 8 weeks of age)

hematopoietic system

enlarged spleen ( J:125805 )

spleen hyperplasia ( J:125805 )

• total cell numbers in inguinal and auxiliary lymph nodes is increased between 5x10⁸ and 25x10⁸ with the greatest numbers observed in the older mice

abnormal erythropoiesis ( J:125805 )

• both single and double transgenic erythrocytes are recovered

• the lymph nodes and spleen become sites of extramedullary hematopoiesis

extramedullary hematopoiesis ( J:125805 )

• the lymph nodes and spleen become sites of extramedullary hematopoiesis

abnormal bone marrow cell morphology/development ( J:125805 )

• bone marrow contains 3.5% R2 gate cells (proerythroblasts) compared to 1% in wild-type mice and twice the number of R3 gate cells (basophilic erythroblasts)

abnormal common myeloid progenitor cell morphology ( J:125805 )

• in a 14 day culture, bone marrow cells produce twice as many colonies as wild-type and single transgenic cells with a bias towards producing colony-forming unit-granulocyte, erythrocyte, monocyte (CFU-GEMM) and erythroid burst colonies

cardiovascular system

lymph node hemorrhage ( J:125805 )

• mice exhibit diffuse hemorrhaging with necrosis in the lymph nodes

cellular

lymph node necrosis ( J:125805 )

growth/size/body

enlarged spleen ( J:125805 )

spleen hyperplasia ( J:125805 )

• total cell numbers in inguinal and auxiliary lymph nodes is increased between 5x10⁸ and 25x10⁸ with the greatest numbers observed in the older mice