About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Itgavtm2Hyn
targeted mutation 2, Richard Hynes
MGI:3759836
Summary 9 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Itga5tm2Hyn/Itga5tm2Hyn
Itgavtm2Hyn/Itgavtm2Hyn
Tg(H2-K1-tsA58)6Kio/0
involves: 129 * C57BL/6 * SJL MGI:4818937
cn2
Gt(ROSA)26Sortm1Sho/0
Itga5tm2Hyn/Itga5tm1Hyn
Itgavtm2Hyn/Itgav+
Tg(Tek-cre)1Ywa/0
involves: 129 * C57BL/6 * SJL MGI:4818939
cn3
Gt(ROSA)26Sortm1Sho/0
Itga5tm2Hyn/Itga5tm1Hyn
Itgavtm1Hyn/Itgavtm2Hyn
Tg(Tek-cre)1Ywa/0
involves: 129 * C57BL/6 * SJL MGI:4818940
cn4
Itgavtm2Hyn/Itgavtm2.1Hyn
Cd19tm1(cre)Cgn/Cd19+
involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6 * FVB MGI:3759846
cn5
Itgavtm2Hyn/Itgavtm2.1Hyn
Lyz2tm1(cre)Ifo/Lyz2+
involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6 * FVB MGI:3759847
cn6
Itgavtm2Hyn/Itgavtm2.1Hyn
Tg(Nes-cre)1Kln/0
involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6 * FVB * SJL MGI:5306156
cn7
Itgavtm2Hyn/Itgavtm2.1Hyn
Tg(GFAP-cre)#Gtm/0
involves: 129P2/OlaHsd * C57BL/6 * CBA MGI:5306155
cn8
Itgavtm2Hyn/Itgavtm2.1Hyn
Tg(Tek-cre)1Ywa/0
involves: 129P2/OlaHsd * C57BL/6 * SJL MGI:5306154
cn9
Itgavtm2Hyn/Itgavtm2.1Hyn
Tg(Tek-cre)1Ywa/?
involves: 129S2/SvPas * C57BL/6 * FVB * SJL MGI:3759849


Genotype
MGI:4818937
cn1
Allelic
Composition
Itga5tm2Hyn/Itga5tm2Hyn
Itgavtm2Hyn/Itgavtm2Hyn
Tg(H2-K1-tsA58)6Kio/0
Genetic
Background
involves: 129 * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itga5tm2Hyn mutation (2 available); any Itga5 mutation (34 available)
Itgavtm2Hyn mutation (2 available); any Itgav mutation (8 available)
Tg(H2-K1-tsA58)6Kio mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• endothelial cells when transiently transfected with cre, have reduced adhesion to fibronectin




Genotype
MGI:4818939
cn2
Allelic
Composition
Gt(ROSA)26Sortm1Sho/0
Itga5tm2Hyn/Itga5tm1Hyn
Itgavtm2Hyn/Itgav+
Tg(Tek-cre)1Ywa/0
Genetic
Background
involves: 129 * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1Sho mutation (4 available); any Gt(ROSA)26Sor mutation (501 available)
Itga5tm1Hyn mutation (1 available); any Itga5 mutation (34 available)
Itga5tm2Hyn mutation (2 available); any Itga5 mutation (34 available)
Itgavtm2Hyn mutation (2 available); any Itgav mutation (8 available)
Tg(Tek-cre)1Ywa mutation (4 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• at P21 fewer mice are alive than wild type

cardiovascular system
• in several of the mice
• ventricular septation defects

cellular
• in several of the mice




Genotype
MGI:4818940
cn3
Allelic
Composition
Gt(ROSA)26Sortm1Sho/0
Itga5tm2Hyn/Itga5tm1Hyn
Itgavtm1Hyn/Itgavtm2Hyn
Tg(Tek-cre)1Ywa/0
Genetic
Background
involves: 129 * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1Sho mutation (4 available); any Gt(ROSA)26Sor mutation (501 available)
Itga5tm1Hyn mutation (1 available); any Itga5 mutation (34 available)
Itga5tm2Hyn mutation (2 available); any Itga5 mutation (34 available)
Itgavtm1Hyn mutation (1 available); any Itgav mutation (8 available)
Itgavtm2Hyn mutation (2 available); any Itgav mutation (8 available)
Tg(Tek-cre)1Ywa mutation (4 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• die at E14.5 of severe dorsal edema and sometimes hemorrhage

cardiovascular system
• aorta vascular ring
• absent ascending aorta
• in one of two surviving mice
• ventricular septation defects

cellular
• in one of two surviving mice




Genotype
MGI:3759846
cn4
Allelic
Composition
Itgavtm2Hyn/Itgavtm2.1Hyn
Cd19tm1(cre)Cgn/Cd19+
Genetic
Background
involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6 * FVB
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd19tm1(cre)Cgn mutation (8 available); any Cd19 mutation (17 available)
Itgavtm2.1Hyn mutation (0 available); any Itgav mutation (8 available)
Itgavtm2Hyn mutation (2 available); any Itgav mutation (8 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
digestive/alimentary system
N
• mice do not develop colitis unlike Itgavtm2Hyn Itgavtm2.1Hyn Tg(Tek-cre)1Ywa mice




Genotype
MGI:3759847
cn5
Allelic
Composition
Itgavtm2Hyn/Itgavtm2.1Hyn
Lyz2tm1(cre)Ifo/Lyz2+
Genetic
Background
involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6 * FVB
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itgavtm2.1Hyn mutation (0 available); any Itgav mutation (8 available)
Itgavtm2Hyn mutation (2 available); any Itgav mutation (8 available)
Lyz2tm1(cre)Ifo mutation (9 available); any Lyz2 mutation (15 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• macrophages exhibit impaired ability to phagocytose and remove apoptotic cells

immune system
• macrophages exhibit impaired ability to phagocytose and remove apoptotic cells
• mice develop colitis similar to Itgavtm2Hyn/Itgavtm2.1Hyn;Tg(Tek-cre)1Ywa mice with similar histology and proinflammatory cytokine expression but at a lower rate (30% compared to 100% of Itgavtm2Hyn/Itgavtm2.1Hyn;Tg(Tek-cre)1Ywa mice at 40 weeks)
• mice contain higher levels of autoantibodies including ones against tropomyosin, phosphotidylserine, double-stranded DNA and anti-nuclear antibodies compared to in Itgavtm2Hyn heterozygote controls

digestive/alimentary system
• mice develop colitis similar to Itgavtm2Hyn/Itgavtm2.1Hyn;Tg(Tek-cre)1Ywa mice with similar histology and proinflammatory cytokine expression but at a lower rate (30% compared to 100% of Itgavtm2Hyn/Itgavtm2.1Hyn;Tg(Tek-cre)1Ywa mice at 40 weeks)

hematopoietic system
• macrophages exhibit impaired ability to phagocytose and remove apoptotic cells




Genotype
MGI:5306156
cn6
Allelic
Composition
Itgavtm2Hyn/Itgavtm2.1Hyn
Tg(Nes-cre)1Kln/0
Genetic
Background
involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6 * FVB * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itgavtm2.1Hyn mutation (0 available); any Itgav mutation (8 available)
Itgavtm2Hyn mutation (2 available); any Itgav mutation (8 available)
Tg(Nes-cre)1Kln mutation (4 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• mice have to be euthanized by 8 months due to urinary dysfunction and bacterial bladder infection
• some mice die by 4 weeks of age
• mice have to be euthanized by 8 months due to urinary dysfunction and bacterial bladder infection

nervous system
N
• mice do not exhibit peripheral nerve abnormalities
• more frequent by 6 months
• vascular abnormalities are initiated in the developing ganglionic eminences of the forebrain
• cerebral blood vessels are tortuous and distended compared to in control mice
• microhemorrhage in the brain at 3 weeks
• at E14.5, mice develop small bilateral cerebral hemorrhages that become progressively more severe throughout embryogenesis unlike control mice
• less severe than in null mice
• microhemorrhage in the spinal cord at 3 weeks
• glial degeneration in the fasciculus gracilis white matter region
• vascular abnormalities are initiated in the developing ganglionic eminences of the forebrain
• in the fasciculus gracilis
• in the fasciculus gracilis
• in the spinal cord white matter

behavior/neurological
• by 2 to 3 weeks, some mice develop motor dysfunction
• by 2 to 3 months, all mice exhibit hind limb coordination defects
• mice exhibit involuntary tail wriggling
• on a rotarod
• mice partially drag hind limbs
• spastic paraparesis by 6 months
• rigid at 2 to 3 months
• rigid at 2 to 3 months
• mice exhibit toe-walking
• mice partially drag hind limbs
• more frequent by 6 months

cardiovascular system
• vascular abnormalities are initiated in the developing ganglionic eminences of the forebrain
• cerebral blood vessels are tortuous and distended compared to in control mice
• microhemorrhage in the brain at 3 weeks
• at E14.5, mice develop small bilateral cerebral hemorrhages that become progressively more severe throughout embryogenesis unlike control mice
• less severe than in null mice
• microhemorrhage in the spinal cord at 3 weeks

muscle
N
• mice do not develop hind limb muscular atrophy
• in hind limb muscles at 2 to 3 months

renal/urinary system
• mice have to be euthanized by 8 months due to urinary dysfunction and bacterial bladder infection

immune system
• mice have to be euthanized by 8 months due to urinary dysfunction and bacterial bladder infection




Genotype
MGI:5306155
cn7
Allelic
Composition
Itgavtm2Hyn/Itgavtm2.1Hyn
Tg(GFAP-cre)#Gtm/0
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itgavtm2.1Hyn mutation (0 available); any Itgav mutation (8 available)
Itgavtm2Hyn mutation (2 available); any Itgav mutation (8 available)
Tg(GFAP-cre)#Gtm mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
• at E18 and P5, mice exhibit microhemorrhage in the cerebellum, mid-brain, and cerebral cortex unlike in control mice
• at E18 and P5, mice exhibit microhemorrhage in the cerebellum unlike in control mice
• at E18 and P5, mice exhibit microhemorrhage in the cerebral cortex unlike in control mice
• between P7 and P14, mice exhibit progressive reduction in cerebral microhemorrhage
• however, adult mice exhibit no hemorrhage or edema in the brain

nervous system
• at E18 and P5, mice exhibit microhemorrhage in the cerebellum, mid-brain, and cerebral cortex unlike in control mice
• at E18 and P5, mice exhibit microhemorrhage in the cerebellum unlike in control mice
• at E18 and P5, mice exhibit microhemorrhage in the cerebral cortex unlike in control mice
• between P7 and P14, mice exhibit progressive reduction in cerebral microhemorrhage
• however, adult mice exhibit no hemorrhage or edema in the brain




Genotype
MGI:5306154
cn8
Allelic
Composition
Itgavtm2Hyn/Itgavtm2.1Hyn
Tg(Tek-cre)1Ywa/0
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itgavtm2.1Hyn mutation (0 available); any Itgav mutation (8 available)
Itgavtm2Hyn mutation (2 available); any Itgav mutation (8 available)
Tg(Tek-cre)1Ywa mutation (4 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
N
• mice exhibit normal cerebral vasculature




Genotype
MGI:3759849
cn9
Allelic
Composition
Itgavtm2Hyn/Itgavtm2.1Hyn
Tg(Tek-cre)1Ywa/?
Genetic
Background
involves: 129S2/SvPas * C57BL/6 * FVB * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itgavtm2.1Hyn mutation (0 available); any Itgav mutation (8 available)
Itgavtm2Hyn mutation (2 available); any Itgav mutation (8 available)
Tg(Tek-cre)1Ywa mutation (4 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• median lifespan is reduced to 44 weeks compared to wild-type mice that live past 80 weeks
• mice die of intestinal constriction

immune system
• mesenteric lymph node dendritic cells cultured with CD4+CD25- T cells produce only 1% regulatory T cells compared to 4% to 5% by control dendritic cells
• CD11chighCD103+ dendritic cells implicated in gut-homing are reduced in the mesenteric lymph nodes compared to in Itgavtm2Hyn heterozygote controls
• however, bone marrow dendritic cells produce normal amount of regulatory T cells when cultured with CD4+CD25- T cells
• the number of regulatory T cells in the colon is decreased by 50% compared to in Itgavtm2Hyn heterozygote controls and are predominantly adaptive regulatory T cells
• the number of regulatory T cells in the spleen and mesenteric lymph nodes is increased compared to in Itgavtm2Hyn heterozygote controls and are predominantly natural regulatory T cells
• Peyer's patches are enlarged and contain an increased proportion of activated CD4+ cells compared to control mice
• mesenteric lymph nodes are enlarged (15+/-1.1x106 cells compared to 7.1+/-0.23 x106 cells in Itgavtm2Hyn heterozygote controls at 3 weeks ,and 34.2+/-6.7 x106 cells compared to 8.6+/-1.6 x106 cells in Itgavtm2Hyn heterozygote controls at 12 weeks) and contain an increased proportion of activated CD4+ cells (20+/-0.81 x106 cells compared to 11+/-1.3 x106 cells in Itgavtm2Hyn heterozygote controls at week 3, and 31.7+/-0.40 x106 cells compared to 15+/-0.81 x106 cells in Itgavtm2Hyn heterozygote controls at week 12)
• macrophages exhibit impaired ability to phagocytose and remove apoptotic cells with twice as many uncleared apoptotic cells present in the intestine
• macrophages are no longer sensitive to the inhibitory effects of RGD peptide
• levels of interferon-gamma in serum and the intestine are elevated compared to in Itgavtm2Hyn heterozygote controls
• IL-4 levels in serum and the intestine and IL-5 and IL-6 levels in the intestine are elevated compared to in Itgavtm2Hyn heterozygote controls
• however, IL-12 and IL-23 levels in the intestine are normal
• TNF-alpha levels in the intestine are elevated compared to in Itgavtm2Hyn heterozygote controls
• mice contain higher levels of autoantibodies including ones against tropomyosin, phosphotidylserine, double-stranded DNA and anti-nuclear antibodies compared to in Itgavtm2Hyn heterozygote controls
• mice develop inflammation in the peritoneum, in the liver, and 40% of mice nasal cavity and respiratory tract
• after 14 weeks of age, mice exhibit inflammation in the colon and cecum with infiltration of lymphocytes, monocytes and plasma cells
• inflammation is chronic and progressive leading to ulcers, acute inflammatory infiltrate and crypt abscesses by week 20 with extensive epithelial proliferation, regeneration and adenocarcinoma from 40 weeks

digestive/alimentary system
• by 20 weeks of age, mice exhibit extensive epithelial proliferation and regeneration
• by 20 weeks of age
• by 20 weeks of age
• after 14 weeks of age, mice exhibit inflammation in the colon and cecum with infiltration of lymphocytes, monocytes and plasma cells
• inflammation is chronic and progressive leading to ulcers, acute inflammatory infiltrate and crypt abscesses by week 20 with extensive epithelial proliferation, regeneration and adenocarcinoma from 40 weeks

neoplasm

growth/size/body
• despite being born with normal weights and no developmental abnormalities, mice begin to lose weight and body condition at 12 weeks of age

hematopoietic system
• macrophages exhibit impaired ability to phagocytose and remove apoptotic cells with twice as many uncleared apoptotic cells present in the intestine
• macrophages are no longer sensitive to the inhibitory effects of RGD peptide
• mesenteric lymph node dendritic cells cultured with CD4+CD25- T cells produce only 1% regulatory T cells compared to 4% to 5% by control dendritic cells
• CD11chighCD103+ dendritic cells implicated in gut-homing are reduced in the mesenteric lymph nodes compared to in Itgavtm2Hyn heterozygote controls
• however, bone marrow dendritic cells produce normal amount of regulatory T cells when cultured with CD4+CD25- T cells
• the number of regulatory T cells in the colon is decreased by 50% compared to in Itgavtm2Hyn heterozygote controls and are predominantly adaptive regulatory T cells
• the number of regulatory T cells in the spleen and mesenteric lymph nodes is increased compared to in Itgavtm2Hyn heterozygote controls and are predominantly natural regulatory T cells

endocrine/exocrine glands
• by 20 weeks of age

homeostasis/metabolism
• levels of interferon-gamma in serum and the intestine are elevated compared to in Itgavtm2Hyn heterozygote controls
• IL-4 levels in serum and the intestine and IL-5 and IL-6 levels in the intestine are elevated compared to in Itgavtm2Hyn heterozygote controls
• however, IL-12 and IL-23 levels in the intestine are normal
• TNF-alpha levels in the intestine are elevated compared to in Itgavtm2Hyn heterozygote controls

cellular
• mesenteric lymph node dendritic cells cultured with CD4+CD25- T cells produce only 1% regulatory T cells compared to 4% to 5% by control dendritic cells
• CD11chighCD103+ dendritic cells implicated in gut-homing are reduced in the mesenteric lymph nodes compared to in Itgavtm2Hyn heterozygote controls
• however, bone marrow dendritic cells produce normal amount of regulatory T cells when cultured with CD4+CD25- T cells
• macrophages exhibit impaired ability to phagocytose and remove apoptotic cells with twice as many uncleared apoptotic cells present in the intestine
• macrophages are no longer sensitive to the inhibitory effects of RGD peptide





Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
10/01/2019
MGI 6.14
The Jackson Laboratory