Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gabrg2tm1Wul mutation
(1 available);
any
Gabrg2 mutation
(41 available)
Tg(Pcp2-cre)2Mpin mutation
(1 available)
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behavior/neurological
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N |
• mice have no motor disabilities, no ataxia or tremor and performed normally on a rotarod test
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• mice are resistant to zolpidem induced sedative and ataxic effects
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nervous system
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• miniature inhibitory postsynaptic currents are absent from all Purkinje cells
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gabrg2tm1Wul mutation
(1 available);
any
Gabrg2 mutation
(41 available)
Tg(Pcp2-cre)2Mpin mutation
(1 available)
Tg(Pcp2-EGFP/Gabrg2)1Wul mutation
(0 available)
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behavior/neurological
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• treatment with zolpiderm does not produce sedation or overt ataxia
• sensitivity to zolpidem is only restored in Purkinje cells
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• following treatment with 3 mg/kg of zolpidem, mice exhibit reduced ability to stay on a rotating rod
• following treatment with zolpidem, mice need more time to cross a hanging beam than do untreated mice
• however, untreated mice have no motor disabilities, no ataxia or tremor and performed normally on a rotarod test and pre-treatment with flumazenil blocks the effects of zolpiderm
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nervous system
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• mice treated with zolpidem exhibit a raise the amplitude and increase the charge of miniature inhibitory postsynaptic currents (mIPSCs) indicating a restoration of zolpiderm-sensitivity but only in Purkinje cells
• due to mosaic expression of the transgene in female mice with integration into the X chromosome, some mice exhibit a lack of mIPSCsdue to mosaic expression of the transgene in female mice with integration into the X chromosome, some mice exhibit a lack of mIPSCs
• however, in GFP+ cells normal zolpidem-sensitivity is restored
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nervous system
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• tuberomammillary nucleus (TMN) neurons lacking GABAA receptors are more excitable than control neurons; more action potentials (APs) are produced for a given current injection and current needed to reach threshold for AP generation is reduced significantly
• tuberomammillary nucleus neurons have increased membrane time constants compared to control neurons
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• whole cell voltage-clamped mutant neurons in the ventral tuberomammillary (VTM) nucleus lack fast GABAA receptor-mediated IPSCs, but are IPSCs are observed in controls
• in dorsal lateral geniculate (DLG) nucleus slice preparations, only 30% of neurons exhibit GABAergic IPSCs compared to most neurons tested in control slices
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behavior/neurological
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N |
• mice appear indistinguishable from controls in health, behavior and appearance
• total sleep-wake time, EEG power spectrum, and duration of REM and non-REM (NREM) periods during the 24 hour cycle do not differ from controls
• habituation to a new environment is not different from controls
• propofol-induced loss of righting reflex (LORR) is not different from controls
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