Phenotypes associated with this allele

• Allele Symbol: Tg(Prnp-ITM2B/APP695*42)A12Emcg
• Allele Name: transgene insertion A12, Eileen McGowan
• Allele ID: MGI:3710689
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Tg(APPSWE)2576Kha/0 Tg(Prnp-ITM2B/APP695*42)A12Emcg/0	involves: C3H * C57BL/6 * SJL	MGI:3710765
tg2	Tg(Prnp-ITM2B/APP695*42)A12Emcg/0	involves: C3H * C57BL/6	MGI:3710763

Genotype
MGI:3710765

cx1

• Allelic Composition: Tg(APPSWE)2576Kha/0; Tg(Prnp-ITM2B/APP695*42)A12Emcg/0
• Genetic Background: involves: C3H * C57BL/6 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

amyloid beta deposits ( J:101023 )

• augmentation of Abeta deposits throughout cortex, hippocampus, and cerebellum is observed in double transgenic mice at 14.5 month-old mice, relative to single transgenics

abnormal forebrain morphology ( J:101023 )

• mice show enhanced senile plaque pathology in the forebrain at 14.5 months

abnormal hindbrain morphology ( J:101023 )

• mice show elevated insoluble Abeta levels in the hindbrain at 14.5 months

homeostasis/metabolism

amyloidosis ( J:101023 )

• formic acid extractable Abeta40 and Abeta42 levels are dramatically elevated at 14.5 months in hindbrain compared Tg(APPSWE)2576Kahs single transgenic mice; levels of Abeta42 are slightly higher than those observed in forebrains of Tg(Prnp-ITM2B/APP695*42)A12Emcg mice at 14.5 months, while Abeta40 levels are significantly higher than in either single transgenic mouse

amyloid beta deposits ( J:101023 )

• augmentation of Abeta deposits throughout cortex, hippocampus, and cerebellum is observed in double transgenic mice at 14.5 month-old mice, relative to single transgenics

Genotype
MGI:3710763

tg2

• Allelic Composition: Tg(Prnp-ITM2B/APP695*42)A12Emcg/0
• Genetic Background: involves: C3H * C57BL/6

nervous system

amyloid beta deposits ( J:101023 )

• cored plaques can be observed as early as 3 months in molecular layer of cerebella of transgenic mice and becoming more pronounced with age; occasional extracellular plaques are seen in the entorhinal/piriform cortices and hippocampus at 6 months of age, but aren't consistently found until >12 months of age

• oldest mice show widespread pathology with cored and diffuse plaques in cerebellum, cortex, hippocampus, and olfactory bulb

• extracellular amyloid plaques show dense amyloid cores with radiating fibrils; many bundles of dystrophic neurites are observed at the periphery of these plaques

• compact plaques range from 38 um in diameter at 3 months to 58 um at 1-18 months

• tau pathology is not evident

• endogenous mouse Abeta is detected in some cored plaques and in diffuse deposits in older mice

abnormal forebrain morphology ( J:101023 )

• forebrain pathology only consistently develops after 12 months of age

gliosis ( J:101023 )

• reactive gliosis is associated with plaques

homeostasis/metabolism

amyloidosis ( J:101023 )

• Abeta1-42 peptide levels in 3-month old transgenic brains are ~1- to 1.5-fold higher than levels in 3- and 6-month oldTg(APPSWE)2576Kahs controls

amyloid beta deposits ( J:101023 )

• cored plaques can be observed as early as 3 months in molecular layer of cerebella of transgenic mice and becoming more pronounced with age; occasional extracellular plaques are seen in the entorhinal/piriform cortices and hippocampus at 6 months of age, but aren't consistently found until >12 months of age

• oldest mice show widespread pathology with cored and diffuse plaques in cerebellum, cortex, hippocampus, and olfactory bulb

• extracellular amyloid plaques show dense amyloid cores with radiating fibrils; many bundles of dystrophic neurites are observed at the periphery of these plaques

• compact plaques range from 38 um in diameter at 3 months to 58 um at 1-18 months

• tau pathology is not evident

• endogenous mouse Abeta is detected in some cored plaques and in diffuse deposits in older mice

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
Alzheimer's disease		DOID:10652		J:101023