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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Gt(ROSA)26Sortm3(SS18/EGFP)Mrc
targeted mutation 3, Mario R Capecchi
MGI:3710098
Summary 7 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
ht1
Gt(ROSA)26Sortm3(SS18/EGFP)Mrc/Gt(ROSA)26Sor+ involves: 129S1/Sv * 129X1/SvJ MGI:3843452
cn2
Gt(ROSA)26Sortm1(cre/ERT)Nat/Gt(ROSA)26Sortm3(SS18/EGFP)Mrc involves: 129 * 129S1/Sv * 129X1/SvJ MGI:3843451
cn3
Gt(ROSA)26Sortm3(SS18/EGFP)Mrc/Gt(ROSA)26Sortm3(SS18/EGFP)Mrc
Myf6tm1(cre)Mrc/Myf6tm1(cre)Mrc
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 MGI:3710997
cn4
Gt(ROSA)26Sortm3(SS18/EGFP)Mrc/Gt(ROSA)26Sortm3(SS18/EGFP)Mrc
Pax7tm1(cre)Mrc/Pax7tm1(cre)Mrc
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 MGI:3710999
cn5
Gt(ROSA)26Sortm3(SS18/EGFP)Mrc/Gt(ROSA)26Sortm3(SS18/EGFP)Mrc
Pax3tm1(cre)Joe/Pax3tm1(cre)Joe
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 MGI:3711000
cn6
Gt(ROSA)26Sortm3(SS18/EGFP)Mrc/Gt(ROSA)26Sortm3(SS18/EGFP)Mrc
Hprttm1(CAG-cre)Mnn/Hprttm1(CAG-cre)Mnn
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 MGI:3711002
cn7
Gt(ROSA)26Sortm3(SS18/EGFP)Mrc/Gt(ROSA)26Sortm3(SS18/EGFP)Mrc
Myf5tm1(cre)Mrc/Myf5tm1(cre)Mrc
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 MGI:3711004


Genotype
MGI:3843452
ht1
Allelic
Composition
Gt(ROSA)26Sortm3(SS18/EGFP)Mrc/Gt(ROSA)26Sor+
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm3(SS18/EGFP)Mrc mutation (0 available); any Gt(ROSA)26Sor mutation (317 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• mouse embryonic fibroblasts exposed to TAT-cre exhibit cell death unlike cells not exposed to TAT-cre




Genotype
MGI:3843451
cn2
Allelic
Composition
Gt(ROSA)26Sortm1(cre/ERT)Nat/Gt(ROSA)26Sortm3(SS18/EGFP)Mrc
Genetic
Background
involves: 129 * 129S1/Sv * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1(cre/ERT)Nat mutation (1 available); any Gt(ROSA)26Sor mutation (317 available)
Gt(ROSA)26Sortm3(SS18/EGFP)Mrc mutation (0 available); any Gt(ROSA)26Sor mutation (317 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• mice treated with tamoxifen exhibit a dosage and frequency dependent lethality dieing by 16 months unlike in mice lacking cre expression

tumorigenesis
• all mice develop tumors in the juxta-articular region in the limbs with some evidence of calcification
• following tamoxifen treatment, all mice develop tumors in the juxta-articular region in the limbs with some evidence of calcification
• mice develop synovial sarcomas
• following tamoxifen treatment, mice develop synovial sarcomas

Mouse Models of Human Disease
OMIM ID Ref(s)
Sarcoma, Synovial 300813 J:147728




Genotype
MGI:3710997
cn3
Allelic
Composition
Gt(ROSA)26Sortm3(SS18/EGFP)Mrc/Gt(ROSA)26Sortm3(SS18/EGFP)Mrc
Myf6tm1(cre)Mrc/Myf6tm1(cre)Mrc
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm3(SS18/EGFP)Mrc mutation (0 available); any Gt(ROSA)26Sor mutation (317 available)
Myf6tm1(cre)Mrc mutation (0 available); any Myf6 mutation (7 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• mice die by 6 months of age

tumorigenesis
N
• unlike in other Gt(ROSA)26Sortm3(SS18)Mrc homozygously activated mice, no tumors form

muscle
• myopathy is characterized by abnormal wavy fiber and limited rhabdomyolysis




Genotype
MGI:3710999
cn4
Allelic
Composition
Gt(ROSA)26Sortm3(SS18/EGFP)Mrc/Gt(ROSA)26Sortm3(SS18/EGFP)Mrc
Pax7tm1(cre)Mrc/Pax7tm1(cre)Mrc
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm3(SS18/EGFP)Mrc mutation (0 available); any Gt(ROSA)26Sor mutation (317 available)
Pax7tm1(cre)Mrc mutation (1 available); any Pax7 mutation (18 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• no pups are born but they can be recovered at E15.5

muscle
• Pax7+ lineage cells are mostly absent except in the proximity to cartilaginous regions of the developing maxilla and nasal turbinates




Genotype
MGI:3711000
cn5
Allelic
Composition
Gt(ROSA)26Sortm3(SS18/EGFP)Mrc/Gt(ROSA)26Sortm3(SS18/EGFP)Mrc
Pax3tm1(cre)Joe/Pax3tm1(cre)Joe
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm3(SS18/EGFP)Mrc mutation (0 available); any Gt(ROSA)26Sor mutation (317 available)
Pax3tm1(cre)Joe mutation (1 available); any Pax3 mutation (31 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• no embryos are recovered at E13.5 but are present at E10.5




Genotype
MGI:3711002
cn6
Allelic
Composition
Gt(ROSA)26Sortm3(SS18/EGFP)Mrc/Gt(ROSA)26Sortm3(SS18/EGFP)Mrc
Hprttm1(CAG-cre)Mnn/Hprttm1(CAG-cre)Mnn
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm3(SS18/EGFP)Mrc mutation (0 available); any Gt(ROSA)26Sor mutation (317 available)
Hprttm1(CAG-cre)Mnn mutation (1 available); any Hprt mutation (1256 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• no pups were recovered

embryogenesis
• at E8.5, embryonic tissue is highly disorganized




Genotype
MGI:3711004
cn7
Allelic
Composition
Gt(ROSA)26Sortm3(SS18/EGFP)Mrc/Gt(ROSA)26Sortm3(SS18/EGFP)Mrc
Myf5tm1(cre)Mrc/Myf5tm1(cre)Mrc
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm3(SS18/EGFP)Mrc mutation (0 available); any Gt(ROSA)26Sor mutation (317 available)
Myf5tm1(cre)Mrc mutation (1 available); any Myf5 mutation (7 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• about 8% of mice die by 2 months of age

tumorigenesis
• at 3 to 5 months, 18 of 18 surviving mice developed multiple tumors (3 to 5 per mouse) compared to over 100 control mice who did not develop tumors at more than 1 year of age
• however, mice that died prior to 2 months of age did not have tumors and did not die from tumor-related causes
• small and potentially metastatic tumors are detected
• most tumors are located within the skeletal muscle in the limbs and intercostals regions with some non-skeletal muscle tissues (such as the cerebellum) harboring tumors
• tumors are vascularized, hemorrhagic and cystic spaces are detected in large tumors
• synovial sarcomas are detected with monophasic (13) outnumbering biphasic (3)
• myxoid and fibrous changes are observed

muscle
• myf5+ lineage cells undergo increased apoptosis ate E11.5 and by E15.5 are absent
• however, myf5+ cells near future rib cartilage and skeletal musculogenesis are normal

growth/size/body
• about 8% of mice are born small

Mouse Models of Human Disease
OMIM ID Ref(s)
Sarcoma, Synovial 300813 J:120967





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last database update
04/19/2016
MGI 6.03
The Jackson Laboratory