Mouse Genome Informatics
ht1
    Gt(ROSA)26Sortm3(SS18/EGFP)Mrc/Gt(ROSA)26Sor+
involves: 129S1/Sv * 129X1/SvJ
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
cellular
• mouse embryonic fibroblasts exposed to TAT-cre exhibit cell death unlike cells not exposed to TAT-cre


Mouse Genome Informatics
cn2
    Gt(ROSA)26Sortm1(cre/ERT)Nat/Gt(ROSA)26Sortm3(SS18/EGFP)Mrc
involves: 129 * 129S1/Sv * 129X1/SvJ
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• mice treated with tamoxifen exhibit a dosage and frequency dependent lethality dieing by 16 months unlike in mice lacking cre expression

tumorigenesis
• all mice develop tumors in the juxta-articular region in the limbs with some evidence of calcification
• following tamoxifen treatment, all mice develop tumors in the juxta-articular region in the limbs with some evidence of calcification
• mice develop synovial sarcomas
• following tamoxifen treatment, mice develop synovial sarcomas

Mouse Models of Human Disease
OMIM IDRef(s)
Sarcoma, Synovial 300813 J:147728


Mouse Genome Informatics
cn3
    Gt(ROSA)26Sortm3(SS18/EGFP)Mrc/Gt(ROSA)26Sortm3(SS18/EGFP)Mrc
Myf6tm1(cre)Mrc/Myf6tm1(cre)Mrc

involves: 129S1/Sv * 129X1/SvJ * C57BL/6
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• mice die by 6 months of age

tumorigenesis
N
• unlike in other Gt(ROSA)26Sortm3(SS18)Mrc homozygously activated mice, no tumors form (J:120967)

muscle
• myopathy is characterized by abnormal wavy fiber and limited rhabdomyolysis


Mouse Genome Informatics
cn4
    Gt(ROSA)26Sortm3(SS18/EGFP)Mrc/Gt(ROSA)26Sortm3(SS18/EGFP)Mrc
Pax7tm1(cre)Mrc/Pax7tm1(cre)Mrc

involves: 129S1/Sv * 129X1/SvJ * C57BL/6
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• no pups are born but they can be recovered at E15.5

muscle
• Pax7+ lineage cells are mostly absent except in the proximity to cartilaginous regions of the developing maxilla and nasal turbinates


Mouse Genome Informatics
cn5
    Gt(ROSA)26Sortm3(SS18/EGFP)Mrc/Gt(ROSA)26Sortm3(SS18/EGFP)Mrc
Pax3tm1(cre)Joe/Pax3tm1(cre)Joe

involves: 129S1/Sv * 129X1/SvJ * C57BL/6
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• no embryos are recovered at E13.5 but are present at E10.5


Mouse Genome Informatics
cn6
    Gt(ROSA)26Sortm3(SS18/EGFP)Mrc/Gt(ROSA)26Sortm3(SS18/EGFP)Mrc
Hprttm1(cre)Mnn/Hprttm1(cre)Mnn

involves: 129S1/Sv * 129X1/SvJ * C57BL/6
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• no pups were recovered (J:120967)

embryogenesis
• at E8.5, embryonic tissue is highly disorganized (J:120967)


Mouse Genome Informatics
cn7
    Gt(ROSA)26Sortm3(SS18/EGFP)Mrc/Gt(ROSA)26Sortm3(SS18/EGFP)Mrc
Myf5tm1(cre)Mrc/Myf5tm1(cre)Mrc

involves: 129S1/Sv * 129X1/SvJ * C57BL/6
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• about 8% of mice die by 2 months of age

tumorigenesis
• at 3 to 5 months, 18 of 18 surviving mice developed multiple tumors (3 to 5 per mouse) compared to over 100 control mice who did not develop tumors at more than 1 year of age
• however, mice that died prior to 2 months of age did not have tumors and did not die from tumor-related causes
• small and potentially metastatic tumors are detected
• most tumors are located within the skeletal muscle in the limbs and intercostals regions with some non-skeletal muscle tissues (such as the cerebellum) harboring tumors
• tumors are vascularized, hemorrhagic and cystic spaces are detected in large tumors
• synovial sarcomas are detected with monophasic (13) outnumbering biphasic (3)
• myxoid and fibrous changes are observed

muscle
• myf5+ lineage cells undergo increased apoptosis ate E11.5 and by E15.5 are absent
• however, myf5+ cells near future rib cartilage and skeletal musculogenesis are normal

growth/size
• about 8% of mice are born small

Mouse Models of Human Disease
OMIM IDRef(s)
Sarcoma, Synovial 300813 J:120967